SASDVH7

Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase (ADAR1) (residues 716-797, i.e. dsRBD3-short)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2024-11-21T15:29:36.436573+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase (ADAR1) (residues 716-797, i.e. dsRBD3-short)

Concentration— – 5.0 Buffer / pH20 mM Na-HEPES, 55 mM KOAc, 10 mM NaCl, 1 mM TCEP / 7.3
Experimental temperature10.0 Facility / beamlineSOLEIL / SWING
Wavelength0.1033 nmExposure0.99 s × 7

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short)
View sequence
GGSAIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENE
proteinHomo sapiensP55265716–797dimerMolecule count 29.236 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11047[3]0.005017093–0.4809656 1/AHas uncertainty columnMissing 0119sasbdb/entries/h7/sasdvh7/source/SASDVH7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)5.47—Å
i0Guinier0.0167064—arbitrary
i0P(r)0.01658—arbitrary
mwExperimental21.2—kDa
mwPorod18.0—kDa
porod_volumePorod27.0—ų
rgGuinier1.920.11Å
rgP(r)1.89—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Na-HEPES, 55 mM KOAc, 10 mM NaCl, 1 mM TCEPBuffer concentration—
pH7.3Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2020-06-27Storage / measurement temperature15.0 / 10.0
Exposure time0.99Frames7
Wavelength0.1033Sample-to-detector distance2.0
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.05 – 4.81Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (4 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:159 field values
Field pathRaw value
codeSASDVH7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDimerization of ADAR1 modulates site-specificity of RNA editing
project.publication.titleDimerization of ADAR1 modulates site-specificity of RNA editing
project.publication.author_listMboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P
project.publication.journalNature Communications
project.publication.doi10.1038/s41467-024-53777-2
project.publication.pmidnull
project.publication.published_date2024 Nov 21
project.statusreleased
project.submitted_date2024-09-19
project.released_date2024-11-21
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVH7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVH7.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVH7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVH7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVH7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVH7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameThird double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short)
experiment.sample.molecule[0].short_namedsRBD3-short
experiment.sample.molecule[0].sequenceGGSAIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENE
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP55265
experiment.sample.molecule[0].uniprot_range_first716
experiment.sample.molecule[0].uniprot_range_last797
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQ TPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLRSQGLQRGFQHPSPRGRSLPQ RGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLA KKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSED LLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK EKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKR ERMQIKRNTNSVPETAPAAIPETKRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLE NRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMP SFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVV INGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQ TPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSV SAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELV RYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQG KQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTF HDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLS LKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLY ISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVP TWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAI CCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILD GTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFK KGLKDMGYGNWISKPQEEKNFYLCPV
experiment.sample.molecule[0].mw9.236
experiment.sample.molecule[0].total_mw18.472
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Na-HEPES, 55 mM KOAc, 10 mM NaCl, 1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.3
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameThird double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase (ADAR1) (residues 716-797, i.e. dsRBD3-short)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameIBPC-CNRS
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_namePierre
experiment.contributor[0].contributor_surnameBarraud
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-4460-8360
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2020-06-27
experiment.storage_temperature15.0
experiment.cell_temperature10.0
experiment.exposure_time0.99
experiment.number_of_frames7
experiment.wavelength0.1033
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.05
experiment.s_max4.81
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDVH7_fit1_model1_img.png
fits[0].models[0].softwarePDB (Protein Data Bank)
fits[0].models[0].pdb_link[0].pdb_code7zj1
fits[0].models[0].pdb_link[0].ext_code7ZJ1
fits[0].models[0].pdb_link[0].ext_typepdb
fits[0].models[0].pdb_link[0].difference_with_modelidentical
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fits[0].models[0].type_of_modelatomic
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fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
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fits[0].models[0].commentnull
fits[0].models[0].user1126
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fits[0].softwareCRYSOL
fits[0].chi_square_value1.298
fits[0].p_value0.0079
fits[0].fit_residual_plotSASDVH7_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDVH7_fit1.fit
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fits[0].software_version2.8.3
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw21.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw18.0
porod_mw_errornull
pddf_i00.01658
pddf_i0_errornull
guinier_i00.0167064
guinier_i0_errornull
pddf_rg1.89
pddf_rg_errornull
guinier_rg1.92
guinier_rg_error0.11
pddf_dmax5.47
pddf_dmax_errornull
porod_volume27.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first15
guinier_point_last137
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVH7_datloglog_img.png
symmetrynull
last_modified2024-11-21T15:29:36.436573+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDVH7
statussuccess
started_at2026-08-11T15:30:41.173915+00:00
finished_at2026-08-11T15:30:50.809396+00:00
source_last_modified2024-11-21T15:29:36.436573+01:00
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View the complete summary.json source
{
  "code": "SASDVH7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
    "publication": {
      "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
      "author_list": "Mboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P",
      "journal": "Nature Communications",
      "doi": "10.1038/s41467-024-53777-2",
      "pmid": null,
      "published_date": "2024 Nov 21"
    },
    "status": "released",
    "submitted_date": "2024-09-19",
    "released_date": "2024-11-21"
  },
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        {
          "long_name": "Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short)",
          "short_name": "dsRBD3-short",
          "sequence": "GGSAIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENE",
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          "uniprot_range_last": 797,
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View the complete manifest.json source
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