Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase ADAR1 (interface mutant, residues 708-801, i.e. ADAR1-dsRBD3 interface mutant)

{
  "code": "SASDVK7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
    "publication": {
      "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
      "author_list": "Mboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P",
      "journal": "Nature Communications",
      "doi": "10.1038/s41467-024-53777-2",
      "pmid": null,
      "published_date": "2024 Nov 21"
    },
    "status": "released",
    "submitted_date": "2024-09-19",
    "released_date": "2024-11-21"
  },
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      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "SOLEIL",
      "city": "Saint-Aubin",
      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "Third double-stranded RNA-binding domain of human ADAR1 (interface mutant, residues 708-801, i.e. ADAR1-dsRBD3 interface mutant)",
          "short_name": "Mutant-ADAR1-dsRBD3",
          "sequence": "MGSSHHHHHHSSGLVPRGSHMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLAQQSGPPHEPKFVYQAKVGGRVFPAVSAHSKKQGKQEAADAALRVLIGENEKAER",
          "organism": "Homo sapiens",
          "uniprot_code": "P55265",
          "uniprot_range_first": 708,
          "uniprot_range_last": 801,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQ\nTPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLRSQGLQRGFQHPSPRGRSLPQ\nRGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLA\nKKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSED\nLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK\nEKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKR\nERMQIKRNTNSVPETAPAAIPETKRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLE\nNRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMP\nSFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVV\nINGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQ\nTPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSV\nSAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELV\nRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQG\nKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTF\nHDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLS\nLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLY\nISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVP\nTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAI\nCCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILD\nGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFK\nKGLKDMGYGNWISKPQEEKNFYLCPV",
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          "total_mw": 12.479,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "construct contains 4 point mutations that render ADAR1-dsRBD3 monomeric, namely V747A+D748Q+W768V+C773S"
        }
      ],
      "buffer": {
        "name": "20 mM Na-phosphate, 100 mM NaCl, 2 mM 2-mercaptoethanol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase ADAR1 (interface mutant, residues 708-801, i.e. ADAR1-dsRBD3 interface mutant)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "IBPC-CNRS",
            "webpage": null
          }
        ],
        "contributor_name": "Pierre",
        "contributor_surname": "Barraud",
        "orcid": "https://orcid.org/0000-0003-4460-8360"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-10-08",
    "storage_temperature": 15.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.99,
    "number_of_frames": 25,
    "wavelength": 0.1033,
    "sample_detector_distance": 2.0,
    "concentration_min": null,
    "concentration_max": 5.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.1,
    "s_max": 3.299,
    "total_exposure_time": null,
    "seccolumn": null
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  "estimated_volume_method": null,
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  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 12.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 12.0,
  "porod_mw_error": null,
  "pddf_i0": 0.01024,
  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 2.21,
  "pddf_rg_error": null,
  "guinier_rg": 2.13,
  "guinier_rg_error": 0.06,
  "pddf_dmax": 7.21,
  "pddf_dmax_error": null,
  "porod_volume": 20.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 44,
  "guinier_point_last": 112,
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  "pddf_point_last": null,
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  "symmetry": null,
  "last_modified": "2024-11-21T15:29:37.065398+01:00",
  "bragg_peak": []
}

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  "finished_at": "2026-08-11T15:32:36.913523+00:00",
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