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SASDVL3

Probable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 12 mg/ml

数据类型：SASBDB 实验数据 状态：Published 曲线类型：Single concentration 最后更新：2026-06-25T09:35:06.471246+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Probable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 12 mg/ml

浓度— – 12.0 缓冲液 / pH20 mM Tris pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM EDTA, 5 mM BME, 5 mM MgCl2 / —
实验温度20.0 设备 / 束线ESRF / BM29
波长0.0999 nm曝光1.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Probable chromosome-partitioning protein ParB (P235G, D237G, K239A)
查看序列
MGMHHHHHHENLYFQGTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSPEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRGAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSDDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDGSGAANVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN
proteinVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Q9KNG72–293dimer分子数 234.078 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1500[3]0.0654265346–5.20714051 1/nm含误差列缺失 00sasbdb/entries/l3/sasdvl3/source/SASDVL3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)26.9—nm
i0Guinier1434.2—arbitrary
i0P(r)1487.0—arbitrary
mwExperimental118.8—kDa
mwPorod150.0—kDa
porod_volumePorod240.3—nm³
rgGuinier5.560.058nm
rgP(r)6.18—nm

这些数值是 SASBDB 来源记录，不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”；未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM EDTA, 5 mM BME, 5 mM MgCl2缓冲液浓度—
pH—添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-07-01储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数10
波长0.0999样品-探测器距离2.81
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.065 – 5.207样品体积 / 流速100.0 / 0.07

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/l3/sasdvl3/source/SASDVL3.zipdownloaded2234535341ee2530791b22a99c2fecd80287c5909a596d39fe8d996ed88ec675d60c8下载查看原文件源站
pddfsasbdb/entries/l3/sasdvl3/source/SASDVL3.outdownloaded48958d67faf78f847ab89d608aab5020446e5980dee4872c9ec7c67e41fef3ccf6794下载查看原文件源站
sascifsasbdb/entries/l3/sasdvl3/source/SASDVL3.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/l3/sasdvl3/source/summary.jsondownloaded61387d5d188a493ed59a7c02f90fedf3dc2f9de23f8b7826cccca6b392638e792c2d下载查看原文件源站
curve：来源记录
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full_entry_zip：来源记录与 ZIP 内部目录（2 项）
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pddf：来源记录
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sascif：来源记录
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summary：来源记录
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  "status": "downloaded",
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全部来源字段（无筛选）

这里自动展开来源记录中的每一个字段，包括空值、列表成员和页面上方已展示过的字段。

summary.json：134 个字段值
字段路径原始值
codeSASDVL3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleMolecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i> Partition Complex
project.publication.titleMolecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i> Partition Complex
project.publication.author_listAnu A, Lata S, Chaudhuri B
project.publication.journalACS Bio & Med Chem Au
project.publication.doi10.1021/acsbiomedchemau.5c00284
project.publication.pmidnull
project.publication.published_date2026 Jun 19
project.statusreleased
project.submitted_date2024-06-28
project.released_date2026-06-25
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVL3.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVL3.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVL3_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVL3.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProbable chromosome-partitioning protein ParB (P235G, D237G, K239A)
experiment.sample.molecule[0].short_namevcParB1-linker
experiment.sample.molecule[0].sequenceMGMHHHHHHENLYFQGTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSPEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRGAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSDDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDGSGAANVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN
experiment.sample.molecule[0].organismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
experiment.sample.molecule[0].uniprot_codeQ9KNG7
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last293
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSP EALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRG AIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSD DVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDPSDAKN VSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN
experiment.sample.molecule[0].mw34.078
experiment.sample.molecule[0].total_mw68.156
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionvcParB1-linker triple mutant at 12 mg/ml. The protein construct used for SAXS contains a non-native polyhistidine tag at the N-terminus (MGMHHHHHHENLYFQG).
experiment.sample.buffer.name20 mM Tris pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM EDTA, 5 mM BME, 5 mM MgCl2
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.phnull
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameProbable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 12 mg/ml
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameCSIR
experiment.contributor[0].affiliation[0].addressChandigarh, India
experiment.contributor[0].affiliation[0].full_nameCSIR-Institute of Microbial Technology
experiment.contributor[0].affiliation[0].webpagehttp://www.imtech.res.in/
experiment.contributor[0].contributor_nameANU
experiment.contributor[0].contributor_surnameSODHI
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-07-01
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames10
experiment.wavelength0.0999
experiment.sample_detector_distance2.81
experiment.concentration_minnull
experiment.concentration_max12.0
experiment.sample_volume100.0
experiment.flow_rate0.07
experiment.s_min0.065
experiment.s_max5.207
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw118.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw150.0
porod_mw_errornull
pddf_i01487.0
pddf_i0_errornull
guinier_i01434.2
guinier_i0_errornull
pddf_rg6.18
pddf_rg_errornull
guinier_rg5.56
guinier_rg_error0.058
pddf_dmax26.9
pddf_dmax_errornull
porod_volume240.3
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first9
guinier_point_last14
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVL3_datloglog_img.png
symmetrynull
last_modified2026-06-25T09:35:06.471246+02:00
bragg_peak[]
manifest.json：34 个字段值
字段路径原始值
codeSASDVL3
statussuccess
started_at2026-08-11T14:03:11.210195+00:00
finished_at2026-08-11T14:03:18.323262+00:00
source_last_modified2026-06-25T09:35:06.471246+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDVL3/
files[0].path/data/saxsdb/sasbdb/entries/l3/sasdvl3/source/summary.json
files[0].size6138
files[0].sha2567d5d188a493ed59a7c02f90fedf3dc2f9de23f8b7826cccca6b392638e792c2d
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDVL3.dat
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files[1].size25750
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files[1].rolecurve
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files[2].size48958
files[2].sha256d67faf78f847ab89d608aab5020446e5980dee4872c9ec7c67e41fef3ccf6794
files[2].rolepddf
files[3].statusnot_available
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDVL3.sascif
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files[3].rolesascif
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files[4].rolefull_entry_zip
files[4].zip_member_count2
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDVL3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Molecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i>\n              Partition Complex",
    "publication": {
      "title": "Molecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i>\n              Partition Complex",
      "author_list": "Anu A, Lata S, Chaudhuri B",
      "journal": "ACS Bio & Med Chem Au",
      "doi": "10.1021/acsbiomedchemau.5c00284",
      "pmid": null,
      "published_date": "2026 Jun 19"
    },
    "status": "released",
    "submitted_date": "2024-06-28",
    "released_date": "2026-06-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVL3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVL3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVL3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVL3.sascif",
  "experiment": {
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        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Probable chromosome-partitioning protein ParB (P235G, D237G, K239A)",
          "short_name": "vcParB1-linker",
          "sequence": "MGMHHHHHHENLYFQGTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSPEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRGAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSDDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDGSGAANVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN",
          "organism": "Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)",
          "uniprot_code": "Q9KNG7",
          "uniprot_range_first": 2,
          "uniprot_range_last": 293,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSP\nEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRG\nAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSD\nDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDPSDAKN\nVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN",
          "mw": 34.078,
          "total_mw": 68.156,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "vcParB1-linker triple mutant at 12 mg/ml. The protein construct used for SAXS contains a non-native polyhistidine tag at the N-terminus (MGMHHHHHHENLYFQG)."
        }
      ],
      "buffer": {
        "name": "20 mM Tris pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM EDTA, 5 mM BME, 5 mM MgCl2",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": null,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Probable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 12 mg/ml",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "CSIR",
            "address": "Chandigarh, India",
            "full_name": "CSIR-Institute of Microbial Technology",
            "webpage": "http://www.imtech.res.in/"
          }
        ],
        "contributor_name": "ANU",
        "contributor_surname": "SODHI",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-07-01",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 10,
    "wavelength": 0.0999,
    "sample_detector_distance": 2.81,
    "concentration_min": null,
    "concentration_max": 12.0,
    "sample_volume": 100.0,
    "flow_rate": 0.07,
    "s_min": 0.065,
    "s_max": 5.207,
    "total_exposure_time": null,
    "seccolumn": null
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}
查看完整 manifest.json 原文
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