SASDVL4

Immunoglobulin light chain H3

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-02-24T14:43:23.785119+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Immunoglobulin light chain H3

浓度— – 3.4 缓冲液 / pH20 mM TrisHCL, 150 mM NaCl / 8.0
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.124423 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Immunoglobulin light chain H3
查看序列
QSVLTQPPSTSGTPGQRVTISCSGSSSNIETNTVNWYQQLPGTAPKLVMHTNNQRPSGVPDRFSGSRSGTSASLAIGGLQSEDEADYFCAAWDDNLNGVIFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS
proteinHomo sapiens——–—dimer分子数 222.738 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12633[3]0.0238562–7.29653 1/nm含误差列缺失 029sasbdb/entries/l4/sasdvl4/source/SASDVL4.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.36—nm
i0Guinier730.088—arbitrary
i0P(r)720.6—arbitrary
mwExperimental45.476—kDa
mwPorod35.0—kDa
porod_volumePorod56.4—nm³
rgGuinier2.590.039nm
rgP(r)2.589—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM TrisHCL, 150 mM NaCl缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-05-12储存 / 测量温度20.0 / 20.0
曝光时间0.05帧数20
波长0.124423样品-探测器距离2.8
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.024 – 7.297样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/l4/sasdvl4/source/SASDVL4.zipdownloaded1506476691182ba9dd1408727fc2146628c1ea587a1f50bb15e28e2c6ba13f3c80aa7b下载查看原文件源站
pddfsasbdb/entries/l4/sasdvl4/source/SASDVL4.outdownloaded96678b01b4237e2d06a0a060b64aa4975351e5a4ece8cddb601c3958db1da8f59a37b下载查看原文件源站
sascifsasbdb/entries/l4/sasdvl4/source/SASDVL4.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/l4/sasdvl4/source/summary.jsondownloaded7303a84e5f1d2d3c86dcae082eaa6536ce9ec6978fa9694accfc4eae3f41908b53de下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:159 个字段值
字段路径原始值
codeSASDVL4
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleA conformational fingerprint for amyloidogenic light chains
project.publication.titleA conformational fingerprint for amyloidogenic light chains
project.publication.author_listPaissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C
project.publication.journaleLife
project.publication.doi10.7554/eLife.102002.3
project.publication.pmidnull
project.publication.published_date2025 Mar 03
project.statusreleased
project.submitted_date2024-07-12
project.released_date2025-02-24
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVL4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVL4.dat
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameImmunoglobulin light chain H3
experiment.sample.molecule[0].short_nameH3
experiment.sample.molecule[0].sequenceQSVLTQPPSTSGTPGQRVTISCSGSSSNIETNTVNWYQQLPGTAPKLVMHTNNQRPSGVPDRFSGSRSGT SASLAIGGLQSEDEADYFCAAWDDNLNGVIFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLI SDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTV APTECS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw22.738
experiment.sample.molecule[0].total_mw45.476
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM TrisHCL, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameImmunoglobulin light chain H3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversità degli studi di Milano
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameFederico
experiment.contributor[0].contributor_surnameBallabio
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-5702-3674
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-05-12
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124423
experiment.sample_detector_distance2.8
experiment.concentration_minnull
experiment.concentration_max3.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.024
experiment.s_max7.297
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareMODELLER
fits[0].models[0].pdb_link[0].pdb_code5mtl
fits[0].models[0].pdb_link[0].ext_code5MTL
fits[0].models[0].pdb_link[0].ext_typepdb
fits[0].models[0].pdb_link[0].difference_with_modelidentical
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_version9.19
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fits[0].models[0].model_mw45.47
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user959
fits[0].fit_unit1/A
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fits[0].softwarePepsi-SAXS
fits[0].chi_square_value1.6058
fits[0].p_value0.0
fits[0].fit_residual_plotSASDVL4_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDVL4_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/H3-H3_saxs.log
fits[0].software_version3
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of immunoglobulin light chain H3 in 20 mM TrisHCL, 150 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.124423 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.40 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw45.476
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw35.0
porod_mw_errornull
pddf_i0720.6
pddf_i0_errornull
guinier_i0730.088
guinier_i0_errornull
pddf_rg2.589
pddf_rg_errornull
guinier_rg2.59
guinier_rg_error0.039
pddf_dmax8.36
pddf_dmax_errornull
porod_volume56.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first29
guinier_point_last145
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVL4_datloglog_img.png
symmetrynull
last_modified2025-02-24T14:43:23.785119+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVL4
statussuccess
started_at2026-08-11T14:26:17.145310+00:00
finished_at2026-08-11T14:26:26.156849+00:00
source_last_modified2025-02-24T14:43:23.785119+01:00
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查看完整 summary.json 原文
{
  "code": "SASDVL4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "A conformational fingerprint for amyloidogenic light chains",
    "publication": {
      "title": "A conformational fingerprint for amyloidogenic light chains",
      "author_list": "Paissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C",
      "journal": "eLife",
      "doi": "10.7554/eLife.102002.3",
      "pmid": null,
      "published_date": "2025 Mar 03"
    },
    "status": "released",
    "submitted_date": "2024-07-12",
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  },
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        }
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      },
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}
查看完整 manifest.json 原文
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