SASDVL5

Small EDRK-rich factor 1 (SERF1a)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2024-08-18T16:49:40.294651+02:00

The sodium phosphate buffer solution contains 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% trifluoroacetic acid (TFA).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Small EDRK-rich factor 1 (SERF1a)

浓度1.0 – 2.0 缓冲液 / pHSodium phosphate buffer / 7.4
实验温度10.0 设备 / 束线NSRRC / TPS13A
波长0.08265 nm曝光2.0 s × 12

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Isoform Short of Small EDRK-rich factor 1
查看序列
MARGNQRELARQKNMKKTQEISKGKRKEDSLTASQRKQRDSEIMQEKQKAANEKKSMQTREK
proteinHomo sapiensO759201–62monomer分子数 17.336 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1697[3]0.01045–1.33889 1/A含误差列缺失 00sasbdb/entries/l5/sasdvl5/source/SASDVL5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.95—Å
i0Guinier0.0187587—1/cm
i0P(r)0.0185—1/cm
mwExperimental9.63—kDa
porod_volumePorod9.1—ų
rgGuinier2.40.01Å
rgP(r)2.37—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称Sodium phosphate buffer缓冲液浓度—
pH7.4添加剂—
缓冲液说明(PB) solution (containing 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% TFA)
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2021-03-11储存 / 测量温度10.0 / 10.0
曝光时间2.0帧数12
波长0.08265样品-探测器距离2.5
光源X-ray synchrotron探测器Eiger X 1M & 9M
机构 / 束线NSRRC / TPS13A · Hsinchu, Taiwan
q 范围0.104 – 13.389样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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sascifsasbdb/entries/l5/sasdvl5/source/SASDVL5.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/l5/sasdvl5/source/summary.jsondownloaded74870afbef6fbdc88c5139455e0b80cffd590acc92852e7dee7006c6e19026cc78aa下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVL5
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleBinding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.
project.publication.titleBinding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.
project.publication.author_listLin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
project.publication.journalIUCrJ
project.publication.doi10.1107/S2052252524006341
project.publication.pmid39120045
project.publication.published_date2024 Sep 1
project.statusreleased
project.submitted_date2024-08-01
project.released_date2024-08-07
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experiment.instrument.detector.resolution0.001
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experiment.instrument.cityHsinchu
experiment.instrument.countryTaiwan
experiment.instrument.beamline_nameTPS13A
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experiment.sample.molecule[0].short_namenull
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experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeO75920
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last62
experiment.sample.molecule[0].oligomerizationmonomer
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experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.nameSodium phosphate buffer
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.comment(PB) solution (containing 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% TFA)
experiment.sample.buffer.additivenull
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experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHsinchu City, Taiwan
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe sodium phosphate buffer solution contains 480 μL of 10 mM PB, pH 7.4, 16.5 μL of 100 mM NaOH, and 10 μL of 1% trifluoroacetic acid (TFA).
experiment_descriptionSynchrotron SAXS data from solutions of Small EDRK-rich factor 1 (SERF1a) in sodium phosphate buffer, pH 7.4 were collected on the TPS13A beam line at the NSRRC (Hsinchu, Taiwan) using a Eiger X 1M & 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 2 mg/ml were measured at 10°C. 12 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unit1/cm
experimental_mw9.63
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
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symmetrynull
last_modified2024-08-18T16:49:40.294651+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVL5
statussuccess
started_at2026-08-11T14:50:39.783084+00:00
finished_at2026-08-11T14:50:46.857758+00:00
source_last_modified2024-08-18T16:49:40.294651+02:00
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查看完整 summary.json 原文
{
  "code": "SASDVL5",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.",
    "publication": {
      "title": "Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.",
      "author_list": "Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US",
      "journal": "IUCrJ",
      "doi": "10.1107/S2052252524006341",
      "pmid": "39120045",
      "published_date": "2024 Sep 1"
    },
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}
查看完整 manifest.json 原文
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