SASDVL7

Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2024-11-21T15:29:46.241348+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)

浓度— – 5.0 缓冲液 / pH20 mM Na-phosphate, 100 mM NaCl, 2 mM 2-mercaptoethanol / 7.0
实验温度10.0 设备 / 束线SOLEIL / SWING
波长0.1033 nm曝光0.99 s × 19

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)
查看序列
MGSSHHHHHHSSGLVPRGSHMMPNKVRKIGELVRYLNTNPVGGLQELAVQKGWRLPEYTVAQESGPPHKREFTITCRVETFVETGSGTSKQVAKRVAAEKLLRKFITENEKAER
proteinHomo sapiens / Xenopus laevisP55265708–801monomer分子数 112.836 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1703[3]0.01003421–0.3298526 1/A含误差列缺失 00sasbdb/entries/l7/sasdvl7/source/SASDVL7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.4—Å
i0Guinier0.011224—arbitrary
i0P(r)0.01124—arbitrary
mwExperimental11.2—kDa
mwPorod11.0—kDa
porod_volumePorod16.0—ų
rgGuinier2.020.1Å
rgP(r)2.07—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Na-phosphate, 100 mM NaCl, 2 mM 2-mercaptoethanol缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2021-10-08储存 / 测量温度15.0 / 10.0
曝光时间0.99帧数19
波长0.1033样品-探测器距离2.0
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.1 – 3.299样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/l7/sasdvl7/source/SASDVL7.outdownloaded638306cff7673990772ac91ffa6351214418d9153ce2ad56927e272b30ed0b88f2cb3下载查看原文件源站
sascifsasbdb/entries/l7/sasdvl7/source/SASDVL7.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/l7/sasdvl7/source/summary.jsondownloaded701747548f94722dd06f6131fef0801d55f3fe1b72b20074d1d88702223847c645d4下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDVL7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDimerization of ADAR1 modulates site-specificity of RNA editing
project.publication.titleDimerization of ADAR1 modulates site-specificity of RNA editing
project.publication.author_listMboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P
project.publication.journalNature Communications
project.publication.doi10.1038/s41467-024-53777-2
project.publication.pmidnull
project.publication.published_date2024 Nov 21
project.statusreleased
project.submitted_date2024-09-19
project.released_date2024-11-21
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVL7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVL7.dat
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVL7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVL7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameChimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)
experiment.sample.molecule[0].short_nameADAR1-ds3/Xlrbpa-ds2
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMMPNKVRKIGELVRYLNTNPVGGLQELAVQKGWRLPEYTVAQESGPPHKREFTITCRVETFVETGSGTSKQVAKRVAAEKLLRKFITENEKAER
experiment.sample.molecule[0].organismHomo sapiens / Xenopus laevis
experiment.sample.molecule[0].uniprot_codeP55265
experiment.sample.molecule[0].uniprot_range_first708
experiment.sample.molecule[0].uniprot_range_last801
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQ TPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLRSQGLQRGFQHPSPRGRSLPQ RGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLA KKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSED LLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK EKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKR ERMQIKRNTNSVPETAPAAIPETKRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLE NRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMP SFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVV INGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQ TPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSV SAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELV RYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQG KQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTF HDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLS LKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLY ISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVP TWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAI CCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILD GTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFK KGLKDMGYGNWISKPQEEKNFYLCPV
experiment.sample.molecule[0].mw12.836
experiment.sample.molecule[0].total_mw12.836
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionChimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2). N-terminal 6xHis-Tag
experiment.sample.buffer.name20 mM Na-phosphate, 100 mM NaCl, 2 mM 2-mercaptoethanol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameChimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameIBPC-CNRS
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_namePierre
experiment.contributor[0].contributor_surnameBarraud
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-4460-8360
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2021-10-08
experiment.storage_temperature15.0
experiment.cell_temperature10.0
experiment.exposure_time0.99
experiment.number_of_frames19
experiment.wavelength0.1033
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.1
experiment.s_max3.299
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw11.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw11.0
porod_mw_errornull
pddf_i00.01124
pddf_i0_errornull
guinier_i00.011224
guinier_i0_errornull
pddf_rg2.07
pddf_rg_errornull
guinier_rg2.02
guinier_rg_error0.1
pddf_dmax6.4
pddf_dmax_errornull
porod_volume16.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first27
guinier_point_last121
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVL7_datloglog_img.png
symmetrynull
last_modified2024-11-21T15:29:46.241348+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVL7
statussuccess
started_at2026-08-11T15:33:21.098113+00:00
finished_at2026-08-11T15:33:28.367554+00:00
source_last_modified2024-11-21T15:29:46.241348+01:00
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查看完整 summary.json 原文
{
  "code": "SASDVL7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
    "publication": {
      "title": "Dimerization of ADAR1 modulates site-specificity of RNA editing",
      "author_list": "Mboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P",
      "journal": "Nature Communications",
      "doi": "10.1038/s41467-024-53777-2",
      "pmid": null,
      "published_date": "2024 Nov 21"
    },
    "status": "released",
    "submitted_date": "2024-09-19",
    "released_date": "2024-11-21"
  },
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      },
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      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
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      "xray_energy": null,
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    "sample": {
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          "long_name": "Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)",
          "short_name": "ADAR1-ds3/Xlrbpa-ds2",
          "sequence": "MGSSHHHHHHSSGLVPRGSHMMPNKVRKIGELVRYLNTNPVGGLQELAVQKGWRLPEYTVAQESGPPHKREFTITCRVETFVETGSGTSKQVAKRVAAEKLLRKFITENEKAER",
          "organism": "Homo sapiens / Xenopus laevis",
          "uniprot_code": "P55265",
          "uniprot_range_first": 708,
          "uniprot_range_last": 801,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQ\nTPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLRSQGLQRGFQHPSPRGRSLPQ\nRGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLA\nKKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSED\nLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIK\nEKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKR\nERMQIKRNTNSVPETAPAAIPETKRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLE\nNRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMP\nSFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVV\nINGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQ\nTPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSV\nSAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELV\nRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQG\nKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTF\nHDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLS\nLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLY\nISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVP\nTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAI\nCCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILD\nGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFK\nKGLKDMGYGNWISKPQEEKNFYLCPV",
          "mw": 12.836,
          "total_mw": 12.836,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2). N-terminal 6xHis-Tag"
        }
      ],
      "buffer": {
        "name": "20 mM Na-phosphate, 100 mM NaCl, 2 mM 2-mercaptoethanol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Chimeric construct between the third dsRBD of human ADAR1 and the second dsRBD of Xenopus Xlrbpa (chimeric construct, residues 708-801, i.e. Chimeric ADAR1-ds3/Xlrbpa-ds2)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "IBPC-CNRS",
            "webpage": null
          }
        ],
        "contributor_name": "Pierre",
        "contributor_surname": "Barraud",
        "orcid": "https://orcid.org/0000-0003-4460-8360"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-10-08",
    "storage_temperature": 15.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.99,
    "number_of_frames": 19,
    "wavelength": 0.1033,
    "sample_detector_distance": 2.0,
    "concentration_min": null,
    "concentration_max": 5.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.1,
    "s_max": 3.299,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 11.2,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 11.0,
  "porod_mw_error": null,
  "pddf_i0": 0.01124,
  "pddf_i0_error": null,
  "guinier_i0": 0.011224,
  "guinier_i0_error": null,
  "pddf_rg": 2.07,
  "pddf_rg_error": null,
  "guinier_rg": 2.02,
  "guinier_rg_error": 0.1,
  "pddf_dmax": 6.4,
  "pddf_dmax_error": null,
  "porod_volume": 16.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 27,
  "guinier_point_last": 121,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVL7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-11-21T15:29:46.241348+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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  "finished_at": "2026-08-11T15:33:28.367554+00:00",
  "source_last_modified": "2024-11-21T15:29:46.241348+01:00",
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