SASDVM2

Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2024-10-07T14:58:22.659398+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 140 mM NaCl / 7.4
实验温度20.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.1127 nm曝光2.0 s × 660

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Neutrophil elastase
查看序列
IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQ
proteinHomo sapiensP0824631–249tetramer分子数 423.295 kDa
Protein map
查看序列
GSTRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSK
proteinStaphylococcus aureus (strain Mu50 / ATCC 700699)Q99QS1374–476dimer分子数 212.056 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1912[3]0.011402–0.472902 1/A含误差列缺失 067sasbdb/entries/m2/sasdvm2/source/SASDVM2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.0—Å
i0Guinier81.8999—arbitrary
i0P(r)82.02—arbitrary
mwExperimental111.4—kDa
mwPorod96.0—kDa
porod_volumePorod163.0—ų
rgGuinier3.6820.241Å
rgP(r)3.67—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 140 mM NaCl缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-06-26储存 / 测量温度4.0 / 20.0
曝光时间2.0帧数660
波长0.1127样品-探测器距离2.1
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.114 – 4.729样品体积 / 流速60.0 / 0.65

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/m2/sasdvm2/source/SASDVM2.datdownloaded74299b948258b4378d4391cd859e312d67e69116fc8580ace3f2439ff09e61ace3751下载查看原文件源站
full_entry_zipsasbdb/entries/m2/sasdvm2/source/SASDVM2.zipdownloaded59600062ec7972a64e6d5e4d6b6b3b69a9a8ae84796d7da96dac256da2a7e5b49f824d下载查看原文件源站
pddfsasbdb/entries/m2/sasdvm2/source/SASDVM2.outdownloaded813850b36a21dcbd62d041f5402b3cbf2808f16ceafd3294ff451e9c0744a7793d684下载查看原文件源站
sascifsasbdb/entries/m2/sasdvm2/source/SASDVM2.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/m2/sasdvm2/source/summary.jsondownloaded8007696c1c1a3963e4fee4a1629acc21bccb083cdf6ca848ac679d53eaa994e3ed8f下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:173 个字段值
字段路径原始值
codeSASDVM2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.author_listMishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
project.publication.journalJ Biol Chem
project.publication.doi10.1016/j.jbc.2024.107627
project.publication.pmid39098536
project.publication.published_date2024 Aug 3
project.statusreleased
project.submitted_date2024-06-17
project.released_date2024-07-22
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVM2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVM2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVM2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVM2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNeutrophil elastase
experiment.sample.molecule[0].short_nameNE
experiment.sample.molecule[0].sequenceIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQ
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP08246
experiment.sample.molecule[0].uniprot_range_first31
experiment.sample.molecule[0].uniprot_range_last249
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLI APNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVI LQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSL CRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVN WIDSIIQRSEDNPCPHPRDPDPASRTH
experiment.sample.molecule[0].mw23.295
experiment.sample.molecule[0].total_mw93.18
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameProtein map
experiment.sample.molecule[1].short_nameEap4
experiment.sample.molecule[1].sequenceGSTRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSK
experiment.sample.molecule[1].organismStaphylococcus aureus (strain Mu50 / ATCC 700699)
experiment.sample.molecule[1].uniprot_codeQ99QS1
experiment.sample.molecule[1].uniprot_range_first374
experiment.sample.molecule[1].uniprot_range_last476
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGT SQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVI DLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFN KNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANL INSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLT SQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEI TVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISD LDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[1].mw12.056
experiment.sample.molecule[1].total_mw24.112
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionEap domain 4 of Protein Map
experiment.sample.buffer.name20 mM HEPES, 140 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTernary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNitin
experiment.contributor[0].contributor_surnameMishra
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8931-7351
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-06-26
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames660
experiment.wavelength0.1127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume60.0
experiment.flow_rate0.65
experiment.s_min0.114
experiment.s_max4.729
experiment.total_exposure_timenull
experiment.seccolumn40
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDVM2_fit1_model1_img.png
fits[0].models[0].softwarePhenix
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDVM2_fit1_model1.pdb
fits[0].models[0].model_mw120.8
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1074
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVM2_fit1_fixed_fit_img.png
fits[0].softwareFoXS
fits[0].chi_square_value1.410757
fits[0].p_value0.0
fits[0].fit_residual_plotSASDVM2_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDVM2_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw111.4
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw96.0
porod_mw_errornull
pddf_i082.02
pddf_i0_errornull
guinier_i081.8999
guinier_i0_errornull
pddf_rg3.67
pddf_rg_errornull
guinier_rg3.682
guinier_rg_error0.241
pddf_dmax11.0
pddf_dmax_errornull
porod_volume163.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last39
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVM2_datloglog_img.png
symmetrynull
last_modified2024-10-07T14:58:22.659398+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVM2
statussuccess
started_at2026-08-11T16:35:10.226246+00:00
finished_at2026-08-11T16:35:20.206214+00:00
source_last_modified2024-10-07T14:58:22.659398+02:00
files[0].statusdownloaded
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files[1].rolecurve
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDVM2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
    "publication": {
      "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
      "author_list": "Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV",
      "journal": "J Biol Chem",
      "doi": "10.1016/j.jbc.2024.107627",
      "pmid": "39098536",
      "published_date": "2024 Aug 3"
    },
    "status": "released",
    "submitted_date": "2024-06-17",
    "released_date": "2024-07-22"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVM2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVM2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVM2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVM2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVM2.sascif",
  "experiment": {
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      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Neutrophil elastase",
          "short_name": "NE",
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          "oligomerization": "tetramer",
          "molecular_type": "protein",
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          "number_molecules": 4,
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          "molecule_source": "biological",
          "molecule_description": ""
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          "uniprot_range_first": 374,
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          "oligomerization": "dimer",
          "molecular_type": "protein",
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      "buffer": {
        "name": "20 mM HEPES, 140 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
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    },
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      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Kansas State University",
            "webpage": null
          }
        ],
        "contributor_name": "Nitin",
        "contributor_surname": "Mishra",
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-06-26",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
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      "software": "FoXS",
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      "p_value": 0.0,
      "fit_residual_plot": "SASDVM2_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVM2_fit1.fit",
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  "description": null,
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  "intensities_log_log_plot": "SASDVM2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-10-07T14:58:22.659398+02:00",
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}
查看完整 manifest.json 原文
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  "started_at": "2026-08-11T16:35:10.226246+00:00",
  "finished_at": "2026-08-11T16:35:20.206214+00:00",
  "source_last_modified": "2024-10-07T14:58:22.659398+02:00",
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