Probable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 8 mg/ml

{
  "code": "SASDVN3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Molecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i>\n              Partition Complex",
    "publication": {
      "title": "Molecular Insights on the Assembling of ParB1 and ParB1- <i>parS1</i>\n              Partition Complex",
      "author_list": "Anu A, Lata S, Chaudhuri B",
      "journal": "ACS Bio & Med Chem Au",
      "doi": "10.1021/acsbiomedchemau.5c00284",
      "pmid": null,
      "published_date": "2026 Jun 19"
    },
    "status": "released",
    "submitted_date": "2024-06-28",
    "released_date": "2026-06-25"
  },
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Probable chromosome-partitioning protein ParB (P235G, D237G, K239A)",
          "short_name": "vcParB1-linker",
          "sequence": "MGMHHHHHHENLYFQGTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSPEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRGAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSDDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDGSGAANVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN",
          "organism": "Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)",
          "uniprot_code": "Q9KNG7",
          "uniprot_range_first": 2,
          "uniprot_range_last": 293,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSP\nEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRG\nAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSD\nDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDPSDAKN\nVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN",
          "mw": 34.078,
          "total_mw": 68.156,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "vcParB1-linker triple mutant  for SAXS batch mode at 8 mg/ml. The protein construct used for SAXS contains a non-native polyhistidine tag at the N-terminus (MGMHHHHHHENLYFQG)."
        }
      ],
      "buffer": {
        "name": "20 mM Tris pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM EDTA, 5 mM BME, 5 mM MgCl2",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": null,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Probable chromosome-partitioning protein ParB linker triple mutant (vcParB1-linker) at 8 mg/ml",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "CSIR",
            "address": "Chandigarh, India",
            "full_name": "CSIR-Institute of Microbial Technology",
            "webpage": "http://www.imtech.res.in/"
          }
        ],
        "contributor_name": "ANU",
        "contributor_surname": "SODHI",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-07-01",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 10,
    "wavelength": 0.0999,
    "sample_detector_distance": 2.81,
    "concentration_min": null,
    "concentration_max": 8.0,
    "sample_volume": 100.0,
    "flow_rate": 0.07,
    "s_min": 0.065,
    "s_max": 5.207,
    "total_exposure_time": null,
    "seccolumn": null
  },
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 94.2,
  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": 150.0,
  "porod_mw_error": null,
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  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 5.588,
  "pddf_rg_error": null,
  "guinier_rg": 5.06,
  "guinier_rg_error": 0.041,
  "pddf_dmax": 24.6,
  "pddf_dmax_error": null,
  "porod_volume": 240.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 3,
  "guinier_point_last": 20,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVN3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-06-25T09:35:09.007732+02:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T14:04:27.288517+00:00",
  "finished_at": "2026-08-11T14:04:34.650119+00:00",
  "source_last_modified": "2026-06-25T09:35:09.007732+02:00",
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