SASDVP9

NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-07-27T15:35:49.138773+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0

Concentration— – — Buffer / pH20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT / 7.0
Experimental temperature25.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.123982 nmExposure0.095 s × 40

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
NAC domain-containing protein 46
View sequence
NAPSTTITTTKQLSRIDSLDNIDHLLDFSSLPPLIDPGFLGQPGPSFSGARQQHDLKPVLHHPTTAPVDNTYLPTQALNFPYHSVHNSGSDFGYGAGSGNNNKGMIKLEHSLVSVSQETGLSSDVNTTATPEISSYPMMMNPAMMDGSKSACDGLDDLIFWEDLYTS
proteinArabidopsis thalianaQ9SQQ6172–338monomerMolecule count 117.969 kDa
Inactive poly [ADP-ribose] polymerase RCD1
View sequence
STTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS
proteinArabidopsis thalianaQ8RY59499–572monomerMolecule count 18.53 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11535[3]0.0232127–4.461236 1/nmHas uncertainty columnMissing 05sasbdb/entries/p9/sasdvp9/source/SASDVP9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)20.56—nm
i0Guinier2462.95—arbitrary
i0P(r)2507.0—arbitrary
mwExperimental21.5—kDa
mwPorod28.0—kDa
porod_volumePorod37.5—nm³
rgGuinier3.8030.04nm
rgP(r)4.158—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTTBuffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-12-01Storage / measurement temperature— / 25.0
Exposure time0.095Frames40
Wavelength0.123982Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 6M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.023 – 4.461Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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sascifsasbdb/entries/p9/sasdvp9/source/SASDVP9.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/p9/sasdvp9/source/summary.jsondownloaded733631f5ca8d903f2ecef518ebd00ac02d6ae28de3e3e1edec30cc2f8fb4b624199eDownloadView source fileSource site
curve:source record
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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDVP9
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleHierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation
project.publication.titleHierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation
project.publication.author_listDue A, Davey N, Thomasen F, Morffy N, Prestel A, Brakti I, O'Shea C, Strader L, Lindorff‐Larsen K, Skriver K, Kragelund B
project.publication.journalProtein Science
project.publication.doi10.1002/pro.70142
project.publication.pmidnull
project.publication.published_date2025 May 15
project.statusreleased
project.submitted_date2024-11-11
project.released_date2025-07-27
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVP9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVP9.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVP9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVP9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNAC domain-containing protein 46
experiment.sample.molecule[0].short_nameANAC046
experiment.sample.molecule[0].sequenceNAPSTTITTTKQLSRIDSLDNIDHLLDFSSLPPLIDPGFLGQPGPSFSGARQQHDLKPVLHHPTTAPVDNTYLPTQALNFPYHSVHNSGSDFGYGAGSGNNNKGMIKLEHSLVSVSQETGLSSDVNTTATPEISSYPMMMNPAMMDGSKSACDGLDDLIFWEDLYTS
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeQ9SQQ6
experiment.sample.molecule[0].uniprot_range_first172
experiment.sample.molecule[0].uniprot_range_last338
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVEEGGVVVNQGGDQEVVDLPPGFRFHPTDEEIITHYLKEKVFNIRFTAAAIGQADLNKN EPWDLPKIAKMGEKEFYFFCQRDRKYPTGMRTNRATVSGYWKATGKDKEIFRGKGCLVGM KKTLVFYTGRAPKGEKTNWVMHEYRLDGKYSYHNLPKTARDEWVVCRVFHKNAPSTTITT TKQLSRIDSLDNIDHLLDFSSLPPLIDPGFLGQPGPSFSGARQQHDLKPVLHHPTTAPVD NTYLPTQALNFPYHSVHNSGSDFGYGAGSGNNNKGMIKLEHSLVSVSQETGLSSDVNTTA TPEISSYPMMMNPAMMDGSKSACDGLDDLIFWEDLYTS
experiment.sample.molecule[0].mw17.969
experiment.sample.molecule[0].total_mw17.969
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameInactive poly [ADP-ribose] polymerase RCD1
experiment.sample.molecule[1].short_nameRCD1-RST
experiment.sample.molecule[1].sequenceSTTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS
experiment.sample.molecule[1].organismArabidopsis thaliana
experiment.sample.molecule[1].uniprot_codeQ8RY59
experiment.sample.molecule[1].uniprot_range_first499
experiment.sample.molecule[1].uniprot_range_last572
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMEAKIVKVLDSSRCEDGFGKKRKRAASYAAYVTGVSCAKLQNVPPPNGQCQIPDKRRRLE GENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNELEEKSA AIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYCHHKCV EDPKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHYDEATE DSCSRKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPVLDVGRF SSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAFIRKSIY GVGIHLTAADCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGGEEYDNGV DDIESPKNYIVWNINMNTHIFPEFVVRFKLSNLPNAEGNLIAKRDNSGVTLEGPKDLPPQ LESNQGARGSGSANSVGSSTTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKK MTRAEFVRKLRVIVGDDLLRSTITTLQNQPKSKEIPGSIRDHEEGAGGL
experiment.sample.molecule[1].mw8.53
experiment.sample.molecule[1].total_mw8.53
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCopenhagen, Denmark
experiment.contributor[0].affiliation[0].full_nameUniversity of Copenhagen
experiment.contributor[0].affiliation[0].webpagehttp://www.ku.dk/
experiment.contributor[0].contributor_nameAmanda
experiment.contributor[0].contributor_surnameDue
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-1905-4311
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-12-01
experiment.storage_temperaturenull
experiment.cell_temperature25.0
experiment.exposure_time0.095
experiment.number_of_frames40
experiment.wavelength0.123982
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.023
experiment.s_max4.461
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw21.5
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw28.0
porod_mw_errornull
pddf_i02507.0
pddf_i0_errornull
guinier_i02462.95
guinier_i0_errornull
pddf_rg4.158
pddf_rg_errornull
guinier_rg3.803
guinier_rg_error0.04
pddf_dmax20.56
pddf_dmax_errornull
porod_volume37.5
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first31
guinier_point_last104
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVP9_datloglog_img.png
symmetrynull
last_modified2025-07-27T15:35:49.138773+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDVP9
statussuccess
started_at2026-08-11T16:19:40.088298+00:00
finished_at2026-08-11T16:19:47.992331+00:00
source_last_modified2025-07-27T15:35:49.138773+02:00
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View the complete summary.json source
{
  "code": "SASDVP9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Hierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation",
    "publication": {
      "title": "Hierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation",
      "author_list": "Due A, Davey N, Thomasen F, Morffy N, Prestel A, Brakti I, O'Shea C, Strader L, Lindorff‐Larsen K, Skriver K, Kragelund B",
      "journal": "Protein Science",
      "doi": "10.1002/pro.70142",
      "pmid": null,
      "published_date": "2025 May 15"
    },
    "status": "released",
    "submitted_date": "2024-11-11",
    "released_date": "2025-07-27"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVP9.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVP9.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVP9_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVP9_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVP9.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "NAC domain-containing protein 46",
          "short_name": "ANAC046",
          "sequence": "NAPSTTITTTKQLSRIDSLDNIDHLLDFSSLPPLIDPGFLGQPGPSFSGARQQHDLKPVLHHPTTAPVDNTYLPTQALNFPYHSVHNSGSDFGYGAGSGNNNKGMIKLEHSLVSVSQETGLSSDVNTTATPEISSYPMMMNPAMMDGSKSACDGLDDLIFWEDLYTS",
          "organism": "Arabidopsis thaliana",
          "uniprot_code": "Q9SQQ6",
          "uniprot_range_first": 172,
          "uniprot_range_last": 338,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MVEEGGVVVNQGGDQEVVDLPPGFRFHPTDEEIITHYLKEKVFNIRFTAAAIGQADLNKN\nEPWDLPKIAKMGEKEFYFFCQRDRKYPTGMRTNRATVSGYWKATGKDKEIFRGKGCLVGM\nKKTLVFYTGRAPKGEKTNWVMHEYRLDGKYSYHNLPKTARDEWVVCRVFHKNAPSTTITT\nTKQLSRIDSLDNIDHLLDFSSLPPLIDPGFLGQPGPSFSGARQQHDLKPVLHHPTTAPVD\nNTYLPTQALNFPYHSVHNSGSDFGYGAGSGNNNKGMIKLEHSLVSVSQETGLSSDVNTTA\nTPEISSYPMMMNPAMMDGSKSACDGLDDLIFWEDLYTS",
          "mw": 17.969,
          "total_mw": 17.969,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Inactive poly [ADP-ribose] polymerase RCD1",
          "short_name": "RCD1-RST",
          "sequence": "STTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS",
          "organism": "Arabidopsis thaliana",
          "uniprot_code": "Q8RY59",
          "uniprot_range_first": 499,
          "uniprot_range_last": 572,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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View the complete manifest.json source
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