SASDVQ4

Immunoglobulin light chain M10

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-02-24T14:43:45.332018+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Immunoglobulin light chain M10

浓度— – 6.7 缓冲液 / pH20 mM TrisHCL, 150 mM NaCl / 8.0
实验温度10.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光2.0 s × 1400

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Immunoglobulin light chain M10
查看序列
QSALTQPASVSGSPGQSITISCTGTSSDVDSSSYVSWYQQHPGKAPKLIIYDVTYRPSGVSNRFSGSKSGNTASLTISGLQAEDEADYYCSSYTYNRVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
proteinHomo sapiens——–—dimer分子数 222.667 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1588[3]0.151187–3.18114 1/nm含误差列缺失 02sasbdb/entries/q4/sasdvq4/source/SASDVQ4.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.3—nm
i0Guinier26.7177—arbitrary
i0P(r)26.73—arbitrary
mwExperimental45.34—kDa
mwPorod35.9—kDa
porod_volumePorod57.5—nm³
rgGuinier2.550.024nm
rgP(r)2.553—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM TrisHCL, 150 mM NaCl缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-03-17储存 / 测量温度10.0 / 10.0
曝光时间2.0帧数1400
波长0.099样品-探测器距离2.8
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.151 – 3.181样品体积 / 流速100.0 / 0.1

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/q4/sasdvq4/source/SASDVQ4.zipdownloaded127487ce8650ba8784fa2a8a7eafa56b64b30fb529b7e11ea83ff8ef9361946a8668f8下载查看原文件源站
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sascifsasbdb/entries/q4/sasdvq4/source/SASDVQ4.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/q4/sasdvq4/source/summary.jsondownloaded7240f3b1614502fdec1a77861d7bb4ad496764b8310a776399d12e33f2932d8f2ec4下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVQ4
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleA conformational fingerprint for amyloidogenic light chains
project.publication.titleA conformational fingerprint for amyloidogenic light chains
project.publication.author_listPaissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C
project.publication.journaleLife
project.publication.doi10.7554/eLife.102002.3
project.publication.pmidnull
project.publication.published_date2025 Mar 03
project.statusreleased
project.submitted_date2024-07-12
project.released_date2025-02-24
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experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameImmunoglobulin light chain M10
experiment.sample.molecule[0].short_nameM10
experiment.sample.molecule[0].sequenceQSALTQPASVSGSPGQSITISCTGTSSDVDSSSYVSWYQQHPGKAPKLIIYDVTYRPSGVSNRFSGSKSG NTASLTISGLQAEDEADYYCSSYTYNRVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISD FYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP TECS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw22.667
experiment.sample.molecule[0].total_mw45.334
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM TrisHCL, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameImmunoglobulin light chain M10
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversità degli studi di Milano
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameFederico
experiment.contributor[0].contributor_surnameBallabio
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-5702-3674
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-03-17
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time2.0
experiment.number_of_frames1400
experiment.wavelength0.099
experiment.sample_detector_distance2.8
experiment.concentration_minnull
experiment.concentration_max6.7
experiment.sample_volume100.0
experiment.flow_rate0.1
experiment.s_min0.151
experiment.s_max3.181
experiment.total_exposure_timenull
experiment.seccolumn47
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fits[0].models[0].softwareAlphaFold
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
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fits[0].models[0].software_version2
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fits[0].models[0].bead_radiusnull
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fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
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fits[0].softwarePepsi-SAXS
fits[0].chi_square_value1.2216
fits[0].p_value0.0003
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fits[0].software_version3
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of immunoglobulin light chain M10 in 20 mM TrisHCL, 150 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 6.7 mg/ml was injected at a 0.10 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 10°C. 1400 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw45.34
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw35.9
porod_mw_errornull
pddf_i026.73
pddf_i0_errornull
guinier_i026.7177
guinier_i0_errornull
pddf_rg2.553
pddf_rg_errornull
guinier_rg2.55
guinier_rg_error0.024
pddf_dmax8.3
pddf_dmax_errornull
porod_volume57.5
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last59
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVQ4_datloglog_img.png
symmetrynull
last_modified2025-02-24T14:43:45.332018+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVQ4
statussuccess
started_at2026-08-11T14:29:07.729943+00:00
finished_at2026-08-11T14:29:15.336669+00:00
source_last_modified2025-02-24T14:43:45.332018+01:00
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查看完整 summary.json 原文
{
  "code": "SASDVQ4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "A conformational fingerprint for amyloidogenic light chains",
    "publication": {
      "title": "A conformational fingerprint for amyloidogenic light chains",
      "author_list": "Paissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C",
      "journal": "eLife",
      "doi": "10.7554/eLife.102002.3",
      "pmid": null,
      "published_date": "2025 Mar 03"
    },
    "status": "released",
    "submitted_date": "2024-07-12",
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  },
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  "last_modified": "2025-02-24T14:43:45.332018+01:00",
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}
查看完整 manifest.json 原文
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