查看完整 summary.json 原文
{
"code": "SASDVU7",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
"publication": {
"title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation.",
"author_list": "Nguyen BV, Özden C, Dong K, Koc OC, Torres-Ocampo AP, Dziedzic N, Flaherty D, Huang J, Sankara S, Abromson NL, Tomchick DR, Fissore RA, Chen J, Garman SC, Stratton MM",
"journal": "Nat Commun",
"doi": "10.1038/s41467-025-63249-w",
"pmid": "41006217",
"published_date": "2025 Sep 26"
},
"status": "released",
"submitted_date": "2024-10-05",
"released_date": "2025-09-03"
},
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},
"name": "Advanced Light Source (ALS)",
"city": "Berkeley, CA",
"country": "USA",
"beamline_name": "12.3.1 (SIBYLS)",
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"type_of_source": "X-ray synchrotron",
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{
"long_name": "Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344)",
"short_name": "CaMKII Exon 14",
"sequence": "TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
"organism": "Homo sapiens",
"uniprot_code": "Q9UQM7",
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"uniprot_range_last": 478,
"oligomerization": "dodecamer",
"molecular_type": "protein",
"uniprot_sequence": "MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
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"number_molecules": 12,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "The linker region only contains Exon 14."
}
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"comment": null,
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"pka": null,
"ph": null,
"deuteration": null
},
"purity_method": null,
"name": "Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 14 as the linker region",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": null,
"full_name": "university of massachusetts amherst",
"webpage": null
}
],
"contributor_name": "Bao",
"contributor_surname": "Nguyen Viet",
"orcid": ""
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-04-17",
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"cell_temperature": 7.0,
"exposure_time": 3.0,
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"wavelength": 0.1127,
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}
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"experiment_description": "Synchrotron SAXS data from solutions of Ca2+/calmodulin-dependent protein kinase II (CaMKII) holoenzyme with Exon 14 as the linker region in 25 mM tris-HCl pH 8.8, 250 mM KCl, 1 mM TCEP, 2% glycerol were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 6 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-800 series column at 7°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"intensities_log_log_plot": "SASDVU7_datloglog_img.png",
"symmetry": null,
"last_modified": "2025-10-07T11:35:25.964047+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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