Fas-activated serine/threonine kinase/phosphoprotein FASTK (76-549)

{
  "code": "SASDVV2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
    "publication": {
      "title": "Human FASTK preferentially binds single-stranded and G-rich RNA.",
      "author_list": "Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW",
      "journal": "FEBS J",
      "doi": "10.1111/febs.70155",
      "pmid": "40598781",
      "published_date": "2025 Jul 1"
    },
    "status": "released",
    "submitted_date": "2024-06-20",
    "released_date": "2024-07-21"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVV2.ift",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVV2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVV2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVV2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVV2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Fas-activated serine/threonine kinase (amino acids 76-549)",
          "short_name": "FASTK",
          "sequence": ">Q14296_FASTKΔN1-75_HUMAN\r\nGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG",
          "organism": "Homo sapiens",
          "uniprot_code": "Q14296",
          "uniprot_range_first": 76,
          "uniprot_range_last": 549,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQP\nCCLGPSKWGDRPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRR\nLGQLLGSRPRPPPVEQVTLQDLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCAL\nEQERRLRLPPKPPPPLQPLLRGGQGLEAALSCPRFLRYPRQHLISSLAEARPEELTPHVM\nVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKLVLPFGRLNYLPLEQQFMPCL\nERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPHALIVRRYLSL\nLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQ\nDLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLV\nLRERWHFCRDGRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQAL\nGLRWGPEGG",
          "mw": 53.308,
          "total_mw": 53.308,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Recombinant His6-SUMO-FASTK(76-549) after SUMO cleavage and SEC purification. Selected SEC fraction was analysed by SEC-SAXS."
        }
      ],
      "buffer": {
        "name": "50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Fas-activated serine/threonine kinase/phosphoprotein FASTK (76-549)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "University of Warsaw",
            "webpage": null
          }
        ],
        "contributor_name": "Daria",
        "contributor_surname": "Dawidziak",
        "orcid": "https://orcid.org/0000-0001-7468-5346"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2020-01-31",
    "storage_temperature": 4.0,
    "cell_temperature": 15.0,
    "exposure_time": 3.0,
    "number_of_frames": 620,
    "wavelength": 0.094,
    "sample_detector_distance": 3.693,
    "concentration_min": null,
    "concentration_max": 8.89,
    "sample_volume": 45.0,
    "flow_rate": 0.08,
    "s_min": 0.026,
    "s_max": 3.4,
    "total_exposure_time": null,
    "seccolumn": 14
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": null,
          "software": "DENSS",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "density",
          "software_version": "accessed on 2023.09.06",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDVV2_fit1_model1.mrc",
          "model_mw": null,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": "20 models were generated and 19 were chosen for refinement with DENSS",
          "user": 1083
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVV2_fit1_fixed_fit_img.png",
      "software": "DENSS",
      "chi_square_value": 0.2223,
      "p_value": 0.9886,
      "fit_residual_plot": "SASDVV2_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVV2_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/FAST_dN1-75_bift_refine.log",
      "software_version": "accessed on 2023.09.06",
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "BioXTAS RAW",
  "pddf_software_version": "BIFT from BioXTAS RAW 2.2.1",
  "i0_calibration_standard": null,
  "description": "To obtain P(r) data were processed with BIFT based on q range (1/A) 0.0046 to 0.1604",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 60.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": 64.1,
  "guinier_i0_mw_error": null,
  "porod_mw": 79.5,
  "porod_mw_error": null,
  "pddf_i0": 0.03321019,
  "pddf_i0_error": 4.2e-05,
  "guinier_i0": 0.0329417,
  "guinier_i0_error": null,
  "pddf_rg": 3.76,
  "pddf_rg_error": 0.18,
  "guinier_rg": 3.58,
  "guinier_rg_error": 0.168,
  "pddf_dmax": 16.27,
  "pddf_dmax_error": 11.1,
  "porod_volume": 95.8,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 5,
  "guinier_point_last": 199,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVV2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-07-21T18:20:34.498857+02:00",
  "bragg_peak": []
}

{
  "code": "SASDVV2",
  "status": "success",
  "started_at": "2026-08-11T13:47:28.901671+00:00",
  "finished_at": "2026-08-11T13:47:40.339798+00:00",
  "source_last_modified": "2025-07-21T18:20:34.498857+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDVV2/",
      "path": "/data/saxsdb/sasbdb/entries/v2/sasdvv2/source/summary.json",
      "size": 7702,
      "sha256": "0e6939fc243a5c1e4992b736668a1706a77df68593b8e0ee42f1998f42157b6e"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDVV2.dat",
      "path": "/data/saxsdb/sasbdb/entries/v2/sasdvv2/source/SASDVV2.dat",
      "size": 130151,
      "sha256": "068fc6a683d546babbf92ed366aba2164e979698786b76934b2f44cdd44156cf",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDVV2.ift",
      "path": "/data/saxsdb/sasbdb/entries/v2/sasdvv2/source/SASDVV2.out",
      "size": 116926,
      "sha256": "4db7ce6d35be36ccc4c4fa3cf5836338de5cc51db9ac01ca1b07b2724c035715",
      "role": "pddf"
    },
    {
      "status": "not_available",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVV2.sascif",
      "path": "/data/saxsdb/sasbdb/entries/v2/sasdvv2/source/SASDVV2.sascif",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDVV2.zip",
      "path": "/data/saxsdb/sasbdb/entries/v2/sasdvv2/source/SASDVV2.zip",
      "size": 1061027,
      "sha256": "0447373dac3514f4a52311c9063647fa95bea37110640668213cd12d3c6b57db",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}