SASDVY2

Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2024-11-13T14:41:49.223343+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

浓度— – 1.0 缓冲液 / pH25 mM potassium phosphate, 50 mM KCl / 6.2
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.124028 nm曝光0.095 s × 40

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
sub-element stem-loop 5a from SARS-CoV-2 5'genomic end
查看序列
GGGCUGCUUACGGUUUCGUCCGUGUUGCAGCCC
RNASevere acute respiratory syndrome coronavirus 2——–—monomer分子数 110.587 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12583[3]0.145473–7.35028 1/nm含误差列缺失 083sasbdb/entries/y2/sasdvy2/source/SASDVY2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.0—nm
i0Guinier4997.02—arbitrary
i0P(r)4982.0—arbitrary
mwExperimental10.528—kDa
mwGuinier I(0)10.6—kDa
rgGuinier1.890.01nm
rgP(r)1.912—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM potassium phosphate, 50 mM KCl缓冲液浓度—
pH6.2添加剂—
缓冲液说明also used with 10 mM Mg2+ or 10 mM Mg2+/5 mM GdnCl
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2020-08-07储存 / 测量温度10.0 / 20.0
曝光时间0.095帧数40
波长0.124028样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.145 – 7.35样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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sascifsasbdb/entries/y2/sasdvy2/source/SASDVY2.sascifnot_available——下载查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
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statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.
project.publication.titleDissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.
project.publication.author_listMertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
project.publication.journalJ Am Chem Soc
project.publication.doi10.1021/jacs.4c08406
project.publication.pmid39442924
project.publication.published_date2024 Nov 6
project.statusreleased
project.submitted_date2024-07-01
project.released_date2024-09-30
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experiment.instrument.countryGermany
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experiment.sample.molecule[0].short_name5_SL5a
experiment.sample.molecule[0].sequencegGG CUG CUU ACG GUU UCG UCC GUG UUG CAG CCc
experiment.sample.molecule[0].organismSevere acute respiratory syndrome coronavirus 2
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequence
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experiment.sample.molecule[0].total_mw10.587
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe first G and terminal C are artificially added to facilitate in vitro transcription and stabilize the stem
experiment.sample.buffer.name25 mM potassium phosphate, 50 mM KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentalso used with 10 mM Mg2+ or 10 mM Mg2+/5 mM GdnCl
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.2
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experiment.sample.nameSub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameGoethe University Frankfurt, Dept. Biosciences
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameAndreas
experiment.contributor[0].contributor_surnameSchlundt
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-2254-7560
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2020-08-07
experiment.storage_temperature10.0
experiment.cell_temperature20.0
experiment.exposure_time0.095
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tags[]
intensity_unitarbitrary
experimental_mw10.528
experimental_mw_errornull
guinier_i0_mw10.6
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last_modified2024-11-13T14:41:49.223343+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVY2
statussuccess
started_at2026-08-11T13:49:38.258937+00:00
finished_at2026-08-11T13:49:46.805589+00:00
source_last_modified2024-11-13T14:41:49.223343+01:00
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查看完整 summary.json 原文
{
  "code": "SASDVY2",
  "status": "Published",
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  "angular_unit": "1/nm",
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    "title": "Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.",
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      "title": "Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2.",
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      "journal": "J Am Chem Soc",
      "doi": "10.1021/jacs.4c08406",
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}
查看完整 manifest.json 原文
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