查看完整 summary.json 原文
{
"code": "SASDVY7",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "Insights into the stereochemical mechanism of the B(12)-dependent radical SAM glutamine C- methyltransferase (QCMT).",
"publication": {
"title": "Insights into the stereochemical mechanism of the B(12)-dependent radical SAM glutamine C- methyltransferase (QCMT).",
"author_list": "Bourdin T, Guillot A, Mauger M, Lefranc B, Gervason S, Glousieau M, Grimaldi S, Leprince J, Thureau A, Benjdia A, Berteau O",
"journal": "Commun Biol",
"doi": "10.1038/s42003-026-10234-8",
"pmid": "42168528",
"published_date": "2026 May 21"
},
"status": "released",
"submitted_date": "2024-05-17",
"released_date": "2026-05-17"
},
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"name": "Eiger 4M",
"resolution": 75.0
},
"name": "SOLEIL",
"city": "Saint-Aubin",
"country": "France",
"beamline_name": "SWING",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
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{
"long_name": "B12-binding domain/radical SAM domain protein, MJ_0865 family",
"short_name": "QCMTMe",
"sequence": "MDITIFSPGIYTYGAMLIGGVLRDAGHEVTLTRTPEVPEGSLLLASLFST\r\nQHLLDPKIRSLVRRHRERGGTVYVGGPVSAVPEIVLRELAPDAVVVGEGE\r\nETVVRLAEEGASETLPGIAYLDGDQAVVTAPAPPAPIDRPLPLIPEDIGS\r\nQSIRGASAYIETHRGCIGGCTFCQVPRFFGREVRSRPLESIIEEVKAFRA\r\nAGAKRLSISGGTGSLYGSHGCEMNPGAFIALLRGMAEVMGPKNVSAPDIR\r\nVDCITDEILDAIRQYTIGWVFFGIESGSNRVLRQMGKGATVREVEEAVER\r\nCREHGLHVAGSFIVGYPGETERDYEATKDLVAALSLDDVFISSAEPIPGT\r\nPLADLVIRTPRDKNPAFMPHTGEYRALHLTESEARCFDLMMHADMYRPQI\r\nRLVTDEVYAAYLAEAKKQGEDIRAATELILRYAQR",
"organism": "Methanoculleus thermophilus",
"uniprot_code": "WP_0669576",
"uniprot_range_first": 1,
"uniprot_range_last": 435,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MDITIFSPGIYTYGAMLIGGVLRDAGHEVTLTRTPEVPEGSLLLASLFSTQHLLDPKIRS\r\nLVRRHRERGGTVYVGGPVSAVPEIVLRELAPDAVVVGEGEETVVRLAEEGASETLPGIAY\r\nLDGDQAVVTAPAPPAPIDRPLPLIPEDIGSQSIRGASAYIETHRGCIGGCTFCQVPRFFG\r\nREVRSRPLESIIEEVKAFRAAGAKRLSISGGTGSLYGSHGCEMNPGAFIALLRGMAEVMG\r\nPKNVSAPDIRVDCITDEILDAIRQYTIGWVFFGIESGSNRVLRQMGKGATVREVEEAVER\r\nCREHGLHVAGSFIVGYPGETERDYEATKDLVAALSLDDVFISSAEPIPGTPLADLVIRTP\r\nRDKNPAFMPHTGEYRALHLTESEARCFDLMMHADMYRPQIRLVTDEVYAAYLAEAKKQGE\r\nDIRAATELILRYAQR",
"mw": 49.962,
"total_mw": 49.962,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "Apo protein with an Fe-S cluster. The protein construct used for SAXS contains an additional amino acid extension at the N-terminus (MASWSHPQFEKSGGGGGENLYFQGH)."
}
],
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"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "Glutamine C‐methyltransferase without cobalamin from Methanoculleus thermophilus",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": "Soleil",
"address": "Saint-Aubin, France",
"full_name": "Soleil Synchrotron",
"webpage": "http://www.synchrotron-soleil.fr/"
}
],
"contributor_name": "Aurélien",
"contributor_surname": "Thureau",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2024-09-18",
"storage_temperature": 6.0,
"cell_temperature": 20.0,
"exposure_time": 0.99,
"number_of_frames": 646,
"wavelength": 0.1033,
"sample_detector_distance": 2.0,
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"sample_volume": 50.0,
"flow_rate": 0.25,
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"seccolumn": 46
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"description": ""
}
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"experiment_description": "Synchrotron SAXS data from solutions of glutamine C‐methyltransferase without cobalamin in 50 mM Tris, 300 mM NaCl, 5% glycerol, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.25 ml/min flow rate onto a Cytiva Superdex 200 5/150 column at 20°C. 646 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"guinier_point_first": 11,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDVY7_fit1_datloglog_img.png",
"symmetry": null,
"last_modified": "2026-05-27T09:45:48.001805+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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