SASDVZ3

SARS-CoV2 RNA pseudoknot at 4 mg/mL

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-02-06T12:01:11.119786+01:00

Note: Possible repulsive interparticle interference present in the sample.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · SARS-CoV2 RNA pseudoknot at 4 mg/mL

浓度— – 4.0 缓冲液 / pH25 mM potassium phosphate, 50 mM KCl / 6.2
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.123982 nm曝光0.095 s × 35

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
SARS-CoV2 RNA pseudoknot
查看序列
GGCGGUGUAAGUGCAGCCCGUCUUACACCGUGCGGCACAGGCACUAGUACUGAUGUCGUAUACAGGGCU
RNASevere acute respiratory syndrome coronavirus 2——–—monomer分子数 122.307 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12650[3]0.0243063–7.36846 1/nm含误差列缺失 087sasbdb/entries/z3/sasdvz3/source/SASDVZ3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.9—nm
i0Guinier0.0550028—1/cm
mwExperimental23.67—kDa
porod_volumePorod29.0—nm³
rgGuinier2.30.004nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM potassium phosphate, 50 mM KCl缓冲液浓度—
pH6.2添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2021-11-22储存 / 测量温度20.0 / 20.0
曝光时间0.095帧数35
波长0.123982样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.024 – 7.368样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/z3/sasdvz3/source/SASDVZ3.outnot_listed——下载查看原文件
sascifsasbdb/entries/z3/sasdvz3/source/SASDVZ3.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/z3/sasdvz3/source/summary.jsondownloaded60981bfa4f6e369f62e341b91d53e3e23b670ce552658160ec235f99dd390962040e下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(1 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDVZ3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleOptimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2
project.publication.titleOptimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2
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project.publication.doi10.1002/anie.202417961
project.publication.pmidnull
project.publication.published_date2025 Feb 05
project.statusreleased
project.submitted_date2024-06-28
project.released_date2025-02-06
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experiment.instrument.detector.resolutionnull
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experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
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experiment.sample.molecule[0].short_nameSARS-CoV2 PK
experiment.sample.molecule[0].sequenceGGCGGUGUAAGUGCAGCCCGUCUUACACCGUGCGGCACAGGCACUAGUACUGAUGUCGUAUACAGGGCU
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experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeRNA
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experiment.sample.molecule[0].total_mw22.307
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experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSARS-CoV2 RNA pseudoknot at 4 mg/mL
experiment.sample.ext_coefficientnull
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experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
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experiment.contributor[0].contributor_nameJan-Niklas
experiment.contributor[0].contributor_surnameTants
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-2455-8135
experiment.concentration_methodnull
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experiment.date2021-11-22
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estimated_volume_methodnull
pddf_softwarenull
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i0_calibration_standardnull
descriptionNote: Possible repulsive interparticle interference present in the sample.
experiment_descriptionSynchrotron SAXS data from solutions of SARS-CoV2 RNA pseudoknot in 25 mM potassium phosphate, 50 mM KCl, pH 6.2 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 35 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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pddf_i0null
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guinier_i0_errornull
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pddf_rg_errornull
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guinier_rg_error0.004
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porod_volume_errornull
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symmetrynull
last_modified2025-02-06T12:01:11.119786+01:00
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manifest.json:31 个字段值
字段路径原始值
codeSASDVZ3
statussuccess
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finished_at2026-08-11T14:12:09.570158+00:00
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查看完整 summary.json 原文
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查看完整 manifest.json 原文
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