Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) triple mutant I12A/F14A/H20A (AIA-A)

{
  "code": "SASDVZ6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism.",
    "publication": {
      "title": "NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism.",
      "author_list": "Brosey CA, Shen R, Tainer JA",
      "journal": "EMBO J",
      "doi": "10.1038/s44318-024-00360-6",
      "pmid": "39806100",
      "published_date": "2025 Jan 13"
    },
    "status": "released",
    "submitted_date": "2024-09-07",
    "released_date": "2025-01-15"
  },
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  "experiment": {
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      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
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    "sample": {
      "molecule": [
        {
          "long_name": "Mitochondrial intermembrane space import and assembly protein 40",
          "short_name": "CHCHD4",
          "sequence": "GPMSYCRQEGKDRAIAVTKEDAETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA\r\nSGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP\r\nAEQAEETAPIEATATKEEEGSS",
          "organism": "Homo sapiens",
          "uniprot_code": "Q8N4Q1",
          "uniprot_range_first": 1,
          "uniprot_range_last": 142,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA\nSGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP\nAEQAEETAPIEATATKEEEGSS",
          "mw": 15.966,
          "total_mw": 15.966,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "This triple-point mutant of CHCHD4 substitutes alanine for I12, F14, and H20 to disrupt interaction of the N-terminus with mitochondrial binding partner AIF. The protein construct used for SAXS contains an additional two non-native amino acids at the N-terminus (GP)."
        }
      ],
      "buffer": {
        "name": "25 mM HEPES, 150 mM NaCl, 2 mM TCEP",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) triple mutant I12A/F14A/H20A (AIA-A)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Texas, USA",
            "full_name": "MD Anderson Cancer Center",
            "webpage": "https://www.mdanderson.org/"
          }
        ],
        "contributor_name": "Chris",
        "contributor_surname": "Brosey",
        "orcid": "https://orcid.org/0000-0002-0990-0262"
      }
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-10-26",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.3,
    "number_of_frames": 33,
    "wavelength": 0.127,
    "sample_detector_distance": 2.0,
    "concentration_min": null,
    "concentration_max": 7.3,
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    "flow_rate": null,
    "s_min": 0.094,
    "s_max": 4.354,
    "total_exposure_time": null,
    "seccolumn": null
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  "estimated_volume_method": null,
  "pddf_software": "ScÅtter",
  "pddf_software_version": "3.0",
  "i0_calibration_standard": null,
  "description": "Experimental molecular weight determined from SEC-MALS. Molecular weight by volume of correlation (Vc) is 19.8 kDa. Real-space PDDF I(0) should be 39.2 based on ScAtter P(r) calculation.",
  "experiment_description": null,
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  "intensity_unit": "arbitrary",
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  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
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  "pddf_i0": 39.2,
  "pddf_i0_error": 0.039,
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  "pddf_rg_error": 0.017,
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  "guinier_rg_error": 0.139,
  "pddf_dmax": 10.5,
  "pddf_dmax_error": null,
  "porod_volume": 61.98,
  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 3,
  "guinier_point_last": 66,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2025-03-05T17:51:35.340594+01:00",
  "bragg_peak": []
}

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