SASDW77

DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-08-01T07:48:37.612084+02:00

Note: The experimentally determined protein mass (SEC-MALS) is overestimated due to the presence of a fluorescent protein in the sample. The light scattering laser operates at a wavelength that excites the fluorescent protein, leading to inaccurate signal measurements.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain

浓度— – 8.0 缓冲液 / pH20 mM Tris, 150 mM NaCL, 3% glycerol / 7.5
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.123985 nm曝光1.0 s × 3000

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
DCV-GFP-St
查看序列
>DGSTMAKSGNYSYKRWVFTINNPTFEDYVHVLEFCTLDNCKFAIVGEEKGANGTPHLQGFLNLRSNARAAALEESLGGRAWLSRARGSDEDNEEYCAKESTYLRVGEPVSKGRSSSGKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHEYVNAAGITGSGGSGAHIVMVDAYKPTKGSGHHHHHHHH
proteinsynthetic construct——–—monomer分子数 141.266 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1814[3]0.16131–2.50088 1/nm含误差列缺失 00sasbdb/entries/77/sasdw77/source/SASDW77.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.10.2nm
i0Guinier4166.37—arbitrary
i0P(r)4135.0—arbitrary
mwExperimental47.4—kDa
mwPorod33.0—kDa
porod_volumePorod54.0—nm³
rgGuinier2.820.022nm
rgP(r)2.839—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150 mM NaCL, 3% glycerol缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-03-18储存 / 测量温度5.0 / 20.0
曝光时间1.0帧数3000
波长0.123985样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.161 – 2.501样品体积 / 流速80.0 / 0.7

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/77/sasdw77/source/SASDW77.zipdownloaded10122007e802aa3f30b4684db2d266cc7357c233fe2735b385986272d206e8e00a0e43下载查看原文件源站
pddfsasbdb/entries/77/sasdw77/source/SASDW77.outdownloaded742801e013f7b5224811a106f532bb10826b45318d241432c33ba3184a5dafdbbc7b6下载查看原文件源站
sascifsasbdb/entries/77/sasdw77/source/SASDW77.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/77/sasdw77/source/summary.jsondownloaded7202206577092838bf02959193103e21a54e3b0f243b0780630731dce6f3cb2b2ec6下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDW77
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleProgrammable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.
project.publication.titleProgrammable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.
project.publication.author_listZhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
project.publication.journalSmall
project.publication.doi10.1002/smll.202502060
project.publication.pmid40442952
project.publication.published_date2025 Jul
project.statusreleased
project.submitted_date2025-02-06
project.released_date2025-08-01
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDW77.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDW77.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW77_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW77_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW77_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW77_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDW77.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDCV-GFP-St
experiment.sample.molecule[0].short_nameDGSt
experiment.sample.molecule[0].sequence>DGSt MAKSGNYSYKRWVFTINNPTFEDYVHVLEFCTLDNCKFAIVGEEKGANGTPHLQGFLNLRSNARAAALEESLGGRAWLSRARGSDEDNEEYCAKESTYLRVGEPVSKGRSSSGKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHEYVNAAGITGSGGSGAHIVMVDAYKPTKGSGHHHHHHHH
experiment.sample.molecule[0].organismsynthetic construct
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw41.266
experiment.sample.molecule[0].total_mw41.266
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionA chimeric protein consisting of DCV, GFP, and SpyTag domain. DCV is an endonuclease ssDNA binding domain originated from Muscovy duck circovirus. GFP is a green fluorescent protein.
experiment.sample.buffer.name20 mM Tris, 150 mM NaCL, 3% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameKI
experiment.contributor[0].affiliation[0].addressLjubljana, Slovenia
experiment.contributor[0].affiliation[0].full_nameKemijski inštitut
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameJaka
experiment.contributor[0].contributor_surnameSnoj
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-03-18
experiment.storage_temperature5.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames3000
experiment.wavelength0.123985
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volume80.0
experiment.flow_rate0.7
experiment.s_min0.161
experiment.s_max2.501
experiment.total_exposure_timenull
experiment.seccolumn47
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDW77_fit1_model1_img.png
fits[0].models[0].softwareMODELLER
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_version10.5
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fits[0].models[0].model_mw41.26
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1027
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDW77_fit1_fixed_fit_img.png
fits[0].softwarePepsi-SAXS
fits[0].chi_square_value1.1095
fits[0].p_value0.0935
fits[0].fit_residual_plotSASDW77_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDW77_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/DCV_GFP_St_set_very_slow-201-1.log
fits[0].software_version3.0
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionNote: The experimentally determined protein mass (SEC-MALS) is overestimated due to the presence of a fluorescent protein in the sample. The light scattering laser operates at a wavelength that excites the fluorescent protein, leading to inaccurate signal measurements.
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw47.4
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw33.0
porod_mw_errornull
pddf_i04135.0
pddf_i0_errornull
guinier_i04166.37
guinier_i0_errornull
pddf_rg2.839
pddf_rg_errornull
guinier_rg2.82
guinier_rg_error0.022
pddf_dmax9.1
pddf_dmax_error0.2
porod_volume54.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first21
guinier_point_last104
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDW77_datloglog_img.png
symmetrynull
last_modified2025-08-01T07:48:37.612084+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDW77
statussuccess
started_at2026-08-11T15:23:51.928495+00:00
finished_at2026-08-11T15:23:59.711868+00:00
source_last_modified2025-08-01T07:48:37.612084+02:00
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查看完整 summary.json 原文
{
  "code": "SASDW77",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.",
    "publication": {
      "title": "Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.",
      "author_list": "Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R",
      "journal": "Small",
      "doi": "10.1002/smll.202502060",
      "pmid": "40442952",
      "published_date": "2025 Jul"
    },
    "status": "released",
    "submitted_date": "2025-02-06",
    "released_date": "2025-08-01"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDW77.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDW77.dat",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW77_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW77_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDW77.sascif",
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        "name": "Pilatus 6M",
        "resolution": null
      },
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      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
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          "organism": "synthetic construct",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 41.266,
          "total_mw": 41.266,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "A chimeric protein consisting of DCV, GFP, and SpyTag domain. DCV is an endonuclease ssDNA binding domain originated from Muscovy duck circovirus. GFP is a green fluorescent protein."
        }
      ],
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        "concentration_unit": null,
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        "deuteration": null
      },
      "purity_method": null,
      "name": "DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain",
      "ext_coefficient": null,
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      "absorbption": null,
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      "mixture": null
    },
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            "short_name": "KI",
            "address": "Ljubljana, Slovenia",
            "full_name": "Kemijski inštitut",
            "webpage": null
          }
        ],
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        "contributor_surname": "Snoj",
        "orcid": null
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    "concentration_unit": null,
    "date": "2024-03-18",
    "storage_temperature": 5.0,
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}
查看完整 manifest.json 原文
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