SASDW82

Perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-02-23T16:31:10.879589+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL

Concentration— – 2.4 Buffer / pH100 mM NaCl, 20 mM Tris, 47% v/v D₂O / 8.0
Experimental temperature25.0 Facility / beamlineILL / D11
Wavelength0.56 nmExposure1500.0 s × 1

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
GlcNAc-binding protein A (perdeuterated)
View sequence
HGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTANHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVILAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGENPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGSGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQKPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPTPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEMAWDKR
proteinVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)Q9KLD524–485monomerMolecule count 151.253 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1322[3]0.001554642–0.4088828 1/AHas uncertainty columnMissing 00sasbdb/entries/82/sasdw82/source/SASDW82.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.7—Å
i0Guinier0.122998—1/cm
i0P(r)0.1215—1/cm
mwExperimental44.0—kDa
rgGuinier3.6990.628Å
rgP(r)3.73—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name100 mM NaCl, 20 mM Tris, 47% v/v D₂OBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2019-09-21Storage / measurement temperature5.0 / 25.0
Exposure time1500.0Frames1
Wavelength0.56Sample-to-detector distance1.7
Sourceneutron sourceDetectorCERCA
Facility / beamlineILL / D11 · Grenoble, France
q range0.016 – 4.089Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDW82
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleTangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.
project.publication.titleTangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.
project.publication.author_listSørensen HV, Montserrat-Canals M, Coder A, Prévost S, Krueger S, Vaaje-Kolstad G, Bjerregaard-Andersen K, Lund R, Krengel U
project.publication.journalACS Appl Mater Interfaces
project.publication.doi10.1021/acsami.5c24418
project.publication.pmid41709583
project.publication.published_date2026 Feb 18
project.statusreleased
project.submitted_date2024-11-19
project.released_date2025-02-09
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDW82.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDW82.dat
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experiment.instrument.detector.type3-He gas detector
experiment.instrument.detector.nameCERCA
experiment.instrument.detector.resolution7.5
experiment.instrument.nameILL
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameD11
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceneutron source
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGlcNAc-binding protein A (perdeuterated)
experiment.sample.molecule[0].short_nameD-GbpA
experiment.sample.molecule[0].sequenceHGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTANHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVILAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGENPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGSGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQKPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPTPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEMAWDKR
experiment.sample.molecule[0].organismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
experiment.sample.molecule[0].uniprot_codeQ9KLD5
experiment.sample.molecule[0].uniprot_range_first24
experiment.sample.molecule[0].uniprot_range_last485
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKKQPKMTAIALILSGISGLAYGHGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQ YEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTAN HVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVI LAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGEN PAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGS GLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQK PLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPT PGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEM AWDKR
experiment.sample.molecule[0].mw51.253
experiment.sample.molecule[0].total_mw51.253
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description97 % average non-exchangeable deuteration through production in E.coli cultures grown in D₂O and deuterated carbon sources.
experiment.sample.buffer.name100 mM NaCl, 20 mM Tris, 47% v/v D₂O
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePerdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameLund University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameHenrik Vinther
experiment.contributor[0].contributor_surnameSørensen
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-3057-5668
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-09-21
experiment.storage_temperature5.0
experiment.cell_temperature25.0
experiment.exposure_time1500.0
experiment.number_of_frames1
experiment.wavelength0.56
experiment.sample_detector_distance1.7
experiment.concentration_minnull
experiment.concentration_max2.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.016
experiment.s_max4.089
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSANS data from solutions of perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH 8 were collected on the D11 beam line at the Institut Laue-Langevin (ILL; Grenoble, France). Data recorded as I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were collected at a sample concentration of 2.4 mg/mL at 25 degrees centigrade using three instrument configurations: Set-up 1: Sample-Detector distance, 1.7 m; Collimation distance, 5.5 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 1500 s. Set-up 2: Sample-Detector distance, 8.0 m; Collimation distance, 8.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 1500 s. Set-up 3: Sample-Detector distance, 38.95 m; Collimation distance, 40.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 2100 s.
tags[]
intensity_unit1/cm
experimental_mw44.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.1215
pddf_i0_errornull
guinier_i00.122998
guinier_i0_errornull
pddf_rg3.73
pddf_rg_errornull
guinier_rg3.699
guinier_rg_error0.628
pddf_dmax12.7
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first23
guinier_point_last104
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDW82_datloglog_img.png
symmetrynull
last_modified2026-02-23T16:31:10.879589+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDW82
statussuccess
started_at2026-08-11T16:51:35.589116+00:00
finished_at2026-08-11T16:51:42.040327+00:00
source_last_modified2026-02-23T16:31:10.879589+01:00
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View the complete summary.json source
{
  "code": "SASDW82",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.",
    "publication": {
      "title": "Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.",
      "author_list": "Sørensen HV, Montserrat-Canals M, Coder A, Prévost S, Krueger S, Vaaje-Kolstad G, Bjerregaard-Andersen K, Lund R, Krengel U",
      "journal": "ACS Appl Mater Interfaces",
      "doi": "10.1021/acsami.5c24418",
      "pmid": "41709583",
      "published_date": "2026 Feb 18"
    },
    "status": "released",
    "submitted_date": "2024-11-19",
    "released_date": "2025-02-09"
  },
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW82_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDW82.sascif",
  "experiment": {
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      "detector": {
        "type": "3-He gas detector",
        "name": "CERCA",
        "resolution": 7.5
      },
      "name": "ILL",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "D11",
      "beam_geometry": null,
      "type_of_source": "neutron source",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "GlcNAc-binding protein A (perdeuterated)",
          "short_name": "D-GbpA",
          "sequence": "HGYVSAVENGVAEGRVTLCKFAANGTGEKNTHCGAIQYEPQSVEGPDGFPVTGPRDGKIASAESALAAALDEQTADRWVKRPIQAGPQTFEWTFTANHVTKDWKYYITKPNWNPNQPLSRDAFDLNPFCVVEGNMVQPPKRVSHECIVPEREGYQVILAVWDVGDTAASFYNVIDVKFDGNGPVLPDWNPAGQIIPSMDLSIGDTVYTRVFDNDGENPAYRTELKIDSETLTKANQWSYALATKINQTQKQQRAGQLNGDQFVPVYGTNPIYLKEGSGLKSVEIGYQIEAPQPEYSLTVSGLAKEYEIGEQPIQLDLTLEAQGEMSAELTVYNHHQKPLASWSQAMTDGELKSITLELSEAKAGHHMLVSRIKDRDGNLQDQQTLDFMLVEPQTPPTPGDYDFVFPNGLKEYVAGTKVLASDGAIYQCKPWPYSGYCQQWTSNATQYQPGTGSHWEMAWDKR",
          "organism": "Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)",
          "uniprot_code": "Q9KLD5",
          "uniprot_range_first": 24,
          "uniprot_range_last": 485,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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          "mw": 51.253,
          "total_mw": 51.253,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "97 % average non-exchangeable deuteration through production in E.coli cultures grown in D₂O and deuterated carbon sources."
        }
      ],
      "buffer": {
        "name": "100 mM NaCl, 20 mM Tris, 47% v/v D₂O",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Lund University",
            "webpage": null
          }
        ],
        "contributor_name": "Henrik Vinther",
        "contributor_surname": "Sørensen",
        "orcid": "https://orcid.org/0000-0003-3057-5668"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2019-09-21",
    "storage_temperature": 5.0,
    "cell_temperature": 25.0,
    "exposure_time": 1500.0,
    "number_of_frames": 1,
    "wavelength": 0.56,
    "sample_detector_distance": 1.7,
    "concentration_min": null,
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    "s_min": 0.016,
    "s_max": 4.089,
    "total_exposure_time": null,
    "seccolumn": null
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  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SANS data from solutions of perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH 8 were collected on the D11 beam line at the Institut Laue-Langevin (ILL; Grenoble, France). Data recorded as I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were collected at a sample concentration of 2.4 mg/mL at 25 degrees centigrade using three instrument configurations: Set-up 1: Sample-Detector distance, 1.7 m; Collimation distance, 5.5 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 1500 s. Set-up 2: Sample-Detector distance, 8.0 m; Collimation distance, 8.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 1500 s. Set-up 3: Sample-Detector distance, 38.95 m; Collimation distance, 40.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 2100 s.",
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  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDW82_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-02-23T16:31:10.879589+01:00",
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}
View the complete manifest.json source
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