SASDWF9

Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-03-28T13:54:53.565877+01:00

The Rg and the MW are not properly estimated as the profile has been truncated to avoid interparticle interactions. This is a profile from a titration series.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM

Concentration1.3 – 20.0 Buffer / pH137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS) / 7.0
Experimental temperature25.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Proliferating cell nuclear antigen
View sequence
GPHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
proteinHomo sapiensP120041–261trimerMolecule count 329.06 kDa
PCNA-associated factor
View sequence
VRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE
proteinHomo sapiensQ150042–111monomerMolecule count 111.855 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11643[3]0.508168–5.0002 1/nmHas uncertainty columnMissing 00sasbdb/entries/f9/sasdwf9/source/SASDWF9.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.0—nm
i0Guinier72.9836—arbitrary
mwExperimental109.0—kDa
mwPorod117.0—kDa
porod_volumePorod187.0—nm³
rgGuinier3.6670.092nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS)Buffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2012-04-23Storage / measurement temperature10.0 / 25.0
Exposure time—Frames—
Wavelength0.124Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.508 – 5.0Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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pddfsasbdb/entries/f9/sasdwf9/source/SASDWF9.outnot_listed——DownloadView source file
sascifsasbdb/entries/f9/sasdwf9/source/SASDWF9.sascifnot_available——DownloadView source fileSource site
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full_entry_zip:source record and ZIP contents (1 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDWF9
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleKDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models
project.publication.titleKDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models
project.publication.author_listGomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
project.publication.journalJournal of Molecular Biology
project.publication.doi10.1016/j.jmb.2025.169103
project.publication.pmidnull
project.publication.published_date2025 Mar
project.statusreleased
project.submitted_date2025-03-02
project.released_date2025-03-28
pddf_datanull
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWF9.dat
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pddf_plotnull
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF9_guinier_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProliferating cell nuclear antigen
experiment.sample.molecule[0].short_namePCNA
experiment.sample.molecule[0].sequenceGPHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY RCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD LDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI KLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK IADMGHLKYYLAPKIEDEEGS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP12004
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last261
experiment.sample.molecule[0].oligomerizationtrimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY RCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD LDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI KLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK IADMGHLKYYLAPKIEDEEGS
experiment.sample.molecule[0].mw29.06
experiment.sample.molecule[0].total_mw87.18
experiment.sample.molecule[0].number_molecules3
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe protein construct used for SAXS contains an additional three non-native amino acids at the N-terminus (GPH).
experiment.sample.molecule[1].long_namePCNA-associated factor
experiment.sample.molecule[1].short_namep15
experiment.sample.molecule[1].sequenceVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK WQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeQ15004
experiment.sample.molecule[1].uniprot_range_first2
experiment.sample.molecule[1].uniprot_range_last111
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK WQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE
experiment.sample.molecule[1].mw11.855
experiment.sample.molecule[1].total_mw11.855
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionDisordered protein.
experiment.sample.buffer.name137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS)
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameProliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressOeiras, Portugal
experiment.contributor[0].affiliation[0].full_nameInstituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa
experiment.contributor[0].affiliation[0].webpagehttp://www.itqb.unl.pt
experiment.contributor[0].contributor_nameTiago
experiment.contributor[0].contributor_surnameCordeiro
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-2663-3220
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2012-04-23
experiment.storage_temperature10.0
experiment.cell_temperature25.0
experiment.exposure_timenull
experiment.number_of_framesnull
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_min1.3
experiment.concentration_max20.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.508
experiment.s_max5.0
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
descriptionThe Rg and the MW are not properly estimated as the profile has been truncated to avoid interparticle interactions. This is a profile from a titration series.
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw109.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw117.0
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i072.9836
guinier_i0_errornull
pddf_rgnull
pddf_rg_errornull
guinier_rg3.667
guinier_rg_error0.092
pddf_dmax11.0
pddf_dmax_errornull
porod_volume187.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last10
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWF9_datloglog_img.png
symmetrynull
last_modified2025-03-28T13:54:53.565877+01:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDWF9
statussuccess
started_at2026-08-11T16:13:59.906836+00:00
finished_at2026-08-11T16:14:05.760610+00:00
source_last_modified2025-03-28T13:54:53.565877+01:00
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View the complete summary.json source
{
  "code": "SASDWF9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models",
    "publication": {
      "title": "KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models",
      "author_list": "Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T",
      "journal": "Journal of Molecular Biology",
      "doi": "10.1016/j.jmb.2025.169103",
      "pmid": null,
      "published_date": "2025 Mar"
    },
    "status": "released",
    "submitted_date": "2025-03-02",
    "released_date": "2025-03-28"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWF9.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF9_dat_img.png",
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  "experiment": {
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        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Proliferating cell nuclear antigen",
          "short_name": "PCNA",
          "sequence": "GPHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY\r\nRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD\r\nLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI\r\nKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK\r\nIADMGHLKYYLAPKIEDEEGS",
          "organism": "Homo sapiens",
          "uniprot_code": "P12004",
          "uniprot_range_first": 1,
          "uniprot_range_last": 261,
          "oligomerization": "trimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY\nRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD\nLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI\nKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK\nIADMGHLKYYLAPKIEDEEGS",
          "mw": 29.06,
          "total_mw": 87.18,
          "number_molecules": 3,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct used for SAXS contains an additional three non-native amino acids at the N-terminus (GPH)."
        },
        {
          "long_name": "PCNA-associated factor",
          "short_name": "p15",
          "sequence": "VRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK\r\nWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE",
          "organism": "Homo sapiens",
          "uniprot_code": "Q15004",
          "uniprot_range_first": 2,
          "uniprot_range_last": 111,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK\nWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE",
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          "total_mw": 11.855,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Disordered protein."
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      ],
      "buffer": {
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM",
      "ext_coefficient": null,
      "contrast": null,
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      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
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            "webpage": "http://www.itqb.unl.pt"
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        "contributor_name": "Tiago",
        "contributor_surname": "Cordeiro",
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    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
    "concentration_min": 1.3,
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    "s_min": 0.508,
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  "fits": [],
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  "description": "The Rg and the MW are not properly estimated as the profile has been truncated to avoid interparticle interactions. This is a profile from a titration series.",
  "experiment_description": null,
  "tags": [],
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View the complete manifest.json source
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