SASDWG8

NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-07-15T11:21:05.911330+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

浓度— – 8.0 缓冲液 / pH20 mM Tris, 150 mM NaCl, / 7.5
实验温度15.0 设备 / 束线Diamond Light Source / B21
波长0.09464 nm曝光3.0 s × 600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
NTF2L domain of Ras GTPase-activating protein-binding protein 1
查看序列
SMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG
protein—Q132831–141dimer分子数 215.964 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12573[3]0.0045–0.34 1/A含误差列缺失 054sasbdb/entries/g8/sasdwg8/source/SASDWG8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.5—Å
i0Guinier0.044775—1/cm
i0P(r)0.04472—1/cm
mwExperimental29.0—kDa
mwPorod31.0—kDa
porod_volumePorod40.0—ų
rgGuinier2.0150.017Å
rgP(r)2.01—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150 mM NaCl,缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-05-02储存 / 测量温度15.0 / 15.0
曝光时间3.0帧数600
波长0.09464样品-探测器距离3.7
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.045 – 3.4样品体积 / 流速60.0 / 0.08

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/g8/sasdwg8/source/SASDWG8.zipdownloaded192648be78c23a14f802e01756d780292becc7a5d915b24a8d7da6c32c5f6b92fdd5e6下载查看原文件源站
pddfsasbdb/entries/g8/sasdwg8/source/SASDWG8.outdownloaded1203552f5b041fa3c2f261a7fa043620baa29fc5b31cc62baf826a70a74c04b45a40ef下载查看原文件源站
sascifsasbdb/entries/g8/sasdwg8/source/SASDWG8.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/g8/sasdwg8/source/summary.jsondownloaded7302d3f34a5582bf9b7a09b95e2fb3fd0af3ba1ff96cd8211a3ca42fc381279766f2下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:168 个字段值
字段路径原始值
codeSASDWG8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation
project.publicationnull
project.statusapproved
project.submitted_date2025-02-19
project.released_date2026-02-19
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWG8.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWG8_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWG8_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWG8_guinier_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNTF2L domain of Ras GTPase-activating protein-binding protein 1
experiment.sample.molecule[0].short_nameNTF2L
experiment.sample.molecule[0].sequenceSMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeQ13283
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last141
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw15.964
experiment.sample.molecule[0].total_mw31.928
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-05-02
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volume60.0
experiment.flow_rate0.08
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn48
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fits[0].models[0].type_of_modeldummy
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fits[0].models[0].model_mw27.576
fits[0].models[0].bead_radius1.75
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fits[0].models[0].symmetryP2
fits[0].models[0].commentnull
fits[0].models[0].user1160
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fits[0].softwareDAMMIF
fits[0].chi_square_value1.049
fits[0].p_value0.4082
fits[0].fit_residual_plotSASDWG8_fit1_fitresiduals_img.png
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fits[0].description
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fits[1].models[0].bead_radiusnull
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fits[1].chi_square_value1.073
fits[1].p_value0.4665
fits[1].fit_residual_plotSASDWG8_fit2_fitresiduals_img.png
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fits[1].fit_lognull
fits[1].software_versionnull
fits[1].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw29.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw31.0
porod_mw_errornull
pddf_i00.04472
pddf_i0_errornull
guinier_i00.044775
guinier_i0_errornull
pddf_rg2.01
pddf_rg_errornull
guinier_rg2.015
guinier_rg_error0.017
pddf_dmax6.5
pddf_dmax_errornull
porod_volume40.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first48
guinier_point_last461
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWG8_datloglog_img.png
symmetrynull
last_modified2026-07-15T11:21:05.911330+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWG8
statussuccess
started_at2026-08-11T15:53:26.463126+00:00
finished_at2026-08-11T15:53:34.958905+00:00
source_last_modified2026-07-15T11:21:05.911330+02:00
files[0].statusdownloaded
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查看完整 summary.json 原文
{
  "code": "SASDWG8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "SAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation",
    "publication": null,
    "status": "approved",
    "submitted_date": "2025-02-19",
    "released_date": "2026-02-19"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWG8.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWG8.dat",
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  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWG8_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWG8.sascif",
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      "country": "UK",
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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    },
    "sample": {
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          "long_name": "NTF2L domain of Ras GTPase-activating protein-binding protein 1",
          "short_name": "NTF2L",
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          "organism": null,
          "uniprot_code": "Q13283",
          "uniprot_range_first": 1,
          "uniprot_range_last": 141,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKE\nIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSV\nANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVS\nNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQ\nTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD\nQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF\nQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAARE\nGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ",
          "mw": 15.964,
          "total_mw": 31.928,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
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查看完整 manifest.json 原文
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