SASDWH8

NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 7.5, 150 mM NaCl

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-07-15T11:21:05.911330+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 7.5, 150 mM NaCl

浓度— – 10.0 缓冲液 / pH20 mM Tris, 150 mM NaCl, / 7.5
实验温度15.0 设备 / 束线Diamond Light Source / B21
波长0.09464 nm曝光3.0 s × 600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
NTF2L domain and IDR1 of stress granule core protein G3BP1
查看序列
GGGSGMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSS
proteinHomo sapiensQ132831–232dimer分子数 226.651 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12571[3]0.005800388–0.34 1/A含误差列缺失 035sasbdb/entries/h8/sasdwh8/source/SASDWH8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.2—Å
i0Guinier0.111615—1/cm
i0P(r)0.1145—1/cm
mwExperimental60.0—kDa
mwPorod67.0—kDa
porod_volumePorod108.0—ų
rgGuinier3.9710.074Å
rgP(r)4.30.015Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150 mM NaCl,缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-12-04储存 / 测量温度15.0 / 15.0
曝光时间3.0帧数600
波长0.09464样品-探测器距离3.7
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.058 – 3.4样品体积 / 流速60.0 / 0.08

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/h8/sasdwh8/source/SASDWH8.zipdownloaded99439d47ddeb7e9bc472c180a50affecc848df00d4812f807d2cb9ee6515bd96cd7bb下载查看原文件源站
pddfsasbdb/entries/h8/sasdwh8/source/SASDWH8.outdownloaded1259914502fde9813bade4f8d1ffe282f773ddfdd9de2a0ea24ab8529cc26c000e7bcf下载查看原文件源站
sascifsasbdb/entries/h8/sasdwh8/source/SASDWH8.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/h8/sasdwh8/source/summary.jsondownloaded6363318f1d12d56901c6cd61fbadcd1b6766a2524298d572d19d67b1ab9c0193c881下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:145 个字段值
字段路径原始值
codeSASDWH8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation
project.publicationnull
project.statusapproved
project.submitted_date2025-02-19
project.released_date2026-02-19
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWH8.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWH8.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWH8_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWH8.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNTF2L domain and IDR1 of stress granule core protein G3BP1
experiment.sample.molecule[0].short_nameNTF2A
experiment.sample.molecule[0].sequenceGGGSG MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG GFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ13283
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last232
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKE IHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSV ANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVS NDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQ TVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF QSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAARE GDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ
experiment.sample.molecule[0].mw26.651
experiment.sample.molecule[0].total_mw53.302
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 7.5, 150 mM NaCl
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-12-04
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volume60.0
experiment.flow_rate0.08
experiment.s_min0.058
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn48
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDWH8_fit1_model1_img.png
fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versiondammif (r13469)
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fits[0].models[0].model_mw64.1
fits[0].models[0].bead_radius2.4
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/0123NTF2A7-10.log
fits[0].models[0].symmetryP2
fits[0].models[0].commentnull
fits[0].models[0].user1160
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWH8_fit1_fixed_fit_img.png
fits[0].softwareDAMMIF
fits[0].chi_square_value1.066
fits[0].p_value0.0027
fits[0].fit_residual_plotSASDWH8_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDWH8_fit1.fir
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw60.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw67.0
porod_mw_errornull
pddf_i00.1145
pddf_i0_errornull
guinier_i00.111615
guinier_i0_errornull
pddf_rg4.3
pddf_rg_error0.015
guinier_rg3.971
guinier_rg_error0.074
pddf_dmax16.2
pddf_dmax_errornull
porod_volume108.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first32
guinier_point_last190
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWH8_datloglog_img.png
symmetrynull
last_modified2026-07-15T11:21:05.911330+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWH8
statussuccess
started_at2026-08-11T15:54:09.283041+00:00
finished_at2026-08-11T15:54:17.413054+00:00
source_last_modified2026-07-15T11:21:05.911330+02:00
files[0].statusdownloaded
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files[0].size6363
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files[1].size119254
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files[1].rolecurve
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDWH8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "SAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation",
    "publication": null,
    "status": "approved",
    "submitted_date": "2025-02-19",
    "released_date": "2026-02-19"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWH8.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWH8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWH8_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH8_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWH8.sascif",
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      "country": "UK",
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      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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      "beam_profile_al": null
    },
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          "long_name": "NTF2L domain and IDR1 of stress granule core protein G3BP1",
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          "uniprot_range_first": 1,
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          "molecular_type": "protein",
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          "mw": 26.651,
          "total_mw": 53.302,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
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        "comment": null,
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        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 7.5, 150 mM NaCl",
      "ext_coefficient": null,
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    "wavelength": 0.09464,
    "sample_detector_distance": 3.7,
    "concentration_min": null,
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    "sample_volume": 60.0,
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    "seccolumn": 48
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}
查看完整 manifest.json 原文
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