SASDWH9

Double-stranded RNA-binding protein Staufen homolog 1

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-06-20T15:51:47.249886+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Double-stranded RNA-binding protein Staufen homolog 1

Concentration— – 5.0 Buffer / pH50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol / 7.0
Experimental temperature20.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.123983 nmExposure1.0 s × 104

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Double-stranded RNA-binding protein Staufen homolog 1 (82-577)
View sequence
GSHMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
proteinHomo sapiensO9579382–577otherMolecule count 155.215 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11386[3]0.142536–4.00073 1/nmHas uncertainty columnMissing 02sasbdb/entries/h9/sasdwh9/source/SASDWH9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.1—nm
i0Guinier487.596—arbitrary
i0P(r)473.0—arbitrary
mwExperimental68.8—kDa
mwPorod57.6—kDa
porod_volumePorod92.09—nm³
rgGuinier5.00.131nm
rgP(r)4.923—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

Fit 1 · EOM/GAJOE ATSAS 2.8.4

χ²: 1.115CorMap p: 0.0402Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1SASDWH9_01195monomermixEOM/RANCH ATSAS 2.8.4—54.81—Model Image Log
2SASDWH9_02984monomermixEOM/RANCH ATSAS 2.8.4—54.81—Model Image Log
3SASDWH9_statistical_flexibilityotherOther [static image] ———Model Image
4SASDWH9_03496eommixEOM/RANCH ATSAS 2.8.4—108.2—Model Image Log
5SASDWH9_04799eommixEOM/RANCH ATSAS 2.8.4—108.2—Model Image Log
6SASDHW9_05558monomermixEOM/RANCH ATSAS 2.8.4—54.81—Model Image Log
7SASDWH9_06759eommixEOM/RANCH ATSAS 2.8.4—108.2—Model Image Log
8SASDWH9_07537eommixEOM/RANCH ATSAS 2.8.4—108.2—Model Image Log
9SASDWH9_07732monomermixEOM/RANCH ATSAS 2.8.4—54.81—Model Image Log
10SASDWH9_08543monomermixEOM/RANCH ATSAS 2.8.4—54.81—Model Image Log
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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanolBuffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2021-04-23Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames104
Wavelength0.123983Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 6M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.143 – 4.001Sample volume / flow rate100.0 / 0.6

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zipsasbdb/entries/h9/sasdwh9/source/SASDWH9.zipdownloaded65355009131c55a8595e2749806dae3ef27ecccb6915a387e96a23cb37b43611222ce9DownloadView source fileSource site
pddfsasbdb/entries/h9/sasdwh9/source/SASDWH9.outdownloaded51160e516b7bc3c8e3eddc9c4d4063f2d5c361a123935f4eca186b55765b5f98f33e4DownloadView source fileSource site
sascifsasbdb/entries/h9/sasdwh9/source/SASDWH9.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/h9/sasdwh9/source/summary.jsondownloaded14111a83fdee84715bdb70f914961c0844bae0e0b359c519ac1ec7e5e0f68640726bfDownloadView source fileSource site
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full_entry_zip:source record and ZIP contents (17 items)
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:273 field values
Field pathRaw value
codeSASDWH9
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.author_listTripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.70669
project.publication.pmid42300088
project.publication.published_date2026 Jul
project.statusreleased
project.submitted_date2025-02-28
project.released_date2026-06-20
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWH9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWH9.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH9_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWH9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWH9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWH9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDouble-stranded RNA-binding protein Staufen homolog 1 (82-577)
experiment.sample.molecule[0].short_namehStau1
experiment.sample.molecule[0].sequenceGSHMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeO95793
experiment.sample.molecule[0].uniprot_range_first82
experiment.sample.molecule[0].uniprot_range_last577
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS ITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF PVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN LNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN AAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE KEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ PTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM VPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH VPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES CHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].mw55.215
experiment.sample.molecule[0].total_mw55.215
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionDouble-stranded RNA-binding protein Staufen homolog 1. The protein construct used for SAXS contains an additional three non-native amino acids at the N-terminus (GSH).
experiment.sample.buffer.name50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDouble-stranded RNA-binding protein Staufen homolog 1
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameCEITEC - Central European Institute of Technology, Masaryk University
experiment.contributor[0].affiliation[0].webpagehttps://www.ceitec.eu/
experiment.contributor[0].contributor_nameTomas
experiment.contributor[0].contributor_surnameKlumpler
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2021-04-23
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames104
experiment.wavelength0.123983
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume100.0
experiment.flow_rate0.6
experiment.s_min0.143
experiment.s_max4.001
experiment.total_exposure_timenull
experiment.seccolumn1
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fits[0].models[0].softwareEOM/RANCH
fits[0].models[0].pdb_link[]
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fits[0].models[0].type_of_modelmix
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fits[0].models[2].pdb_link[]
fits[0].models[2].model_titleSASDWH9_statistical_flexibility
fits[0].models[2].type_of_modelother
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fits[0].models[2].model_mwnull
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fits[0].models[2].lognull
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fits[0].models[2].user195
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fits[0].models[3].softwareEOM/RANCH
fits[0].models[3].pdb_link[]
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fits[0].models[3].type_of_modelmix
fits[0].models[3].software_versionATSAS 2.8.4
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fits[0].models[3].model_mw108.2
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fits[0].models[3].user195
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fits[0].models[5].pdb_link[]
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fits[0].models[6].model_mw108.2
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fits[0].models[7].softwareEOM/RANCH
fits[0].models[7].pdb_link[]
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View the complete summary.json source
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    "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
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      "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
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View the complete manifest.json source
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