SASDWJ8

NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 6.0, 50 mM NaCl

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-07-15T11:21:05.911330+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 6.0, 50 mM NaCl

Concentration— – 10.0 Buffer / pH50 mM MES, 50 mM NaCl, / 6.0
Experimental temperature15.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.09464 nmExposure3.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
NTF2L domain and IDR1 of stress granule core protein G3BP1
View sequence
GGGSGMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSS
proteinHomo sapiensQ132831–232dimerMolecule count 226.651 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12581[3]0.0045–0.34 1/AHas uncertainty columnMissing 039sasbdb/entries/j8/sasdwj8/source/SASDWJ8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.1—Å
i0Guinier0.113427—1/cm
i0P(r)0.1153—1/cm
mwExperimental53.4—kDa
mwPorod60.0—kDa
porod_volumePorod96.0—ų
rgGuinier3.5950.063Å
rgP(r)3.88—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM MES, 50 mM NaCl,Buffer concentration—
pH6.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-12-04Storage / measurement temperature15.0 / 15.0
Exposure time3.0Frames600
Wavelength0.09464Sample-to-detector distance3.7
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4Sample volume / flow rate60.0 / 0.08

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:145 field values
Field pathRaw value
codeSASDWJ8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation
project.publicationnull
project.statusapproved
project.submitted_date2025-02-19
project.released_date2026-02-19
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNTF2L domain and IDR1 of stress granule core protein G3BP1
experiment.sample.molecule[0].short_nameNTF2A
experiment.sample.molecule[0].sequenceGGGSG MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFG GFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ13283
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last232
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKE IHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSV ANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVS NDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQ TVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF QSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAARE GDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ
experiment.sample.molecule[0].mw26.651
experiment.sample.molecule[0].total_mw53.302
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name50 mM MES, 50 mM NaCl,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 6.0, 50 mM NaCl
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-12-04
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volume60.0
experiment.flow_rate0.08
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn53
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fits[0].chi_square_value1.215
fits[0].p_value0.0061
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estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw53.4
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw60.0
porod_mw_errornull
pddf_i00.1153
pddf_i0_errornull
guinier_i00.113427
guinier_i0_errornull
pddf_rg3.88
pddf_rg_errornull
guinier_rg3.595
guinier_rg_error0.063
pddf_dmax15.1
pddf_dmax_errornull
porod_volume96.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first42
guinier_point_last206
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWJ8_datloglog_img.png
symmetrynull
last_modified2026-07-15T11:21:05.911330+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDWJ8
statussuccess
started_at2026-08-11T15:54:48.614161+00:00
finished_at2026-08-11T15:54:56.908711+00:00
source_last_modified2026-07-15T11:21:05.911330+02:00
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View the complete summary.json source
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  "code": "SASDWJ8",
  "status": "Published",
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  "angular_unit": "1/A",
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    "publication": null,
    "status": "approved",
    "submitted_date": "2025-02-19",
    "released_date": "2026-02-19"
  },
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View the complete manifest.json source
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