SASDWK5

Listeria monocytogenes Chitinase (ChiA)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2025-10-20T08:59:40.863279+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Listeria monocytogenes Chitinase (ChiA)

Concentration— – 10.0 Buffer / pH20 mM Tris, 200 mM NaCl / 8.0
Experimental temperature25.0 Facility / beamlineDiamond Light Source / B21
Wavelength1.0 nmExposure0.5 s × 620

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
chitinase
View sequence
MVATDDASVMPDISNKQVLVGYWHSWKSSGKDGYQQGTSADIALKDTPKAYNVVDVSFMKGDGVNRIPTFKPVGINDSDFRAQVGALNKEGRAVLLALGGADGHVELKAGDEEAFANEIIRQVETYGFDGLDIDLEQSAITAGDNKTVIPAALKIVKDHYKAEGKNFLITMAPEFPYLKPGSAYESYLTSLANYYDYIAPQLYNQGGDGVWVDETNQWIAQNNDTLKESFLYYMADSFINGTRGYLKIPANKFVFGLPANVDAAATGYVTDPQIVKNVFTRLQAKGTPVKGIMTWSVNWDAGKNKAGVPYNNSFSNAYGPIVGTKLEHHHHHH
proteinListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)Q8Y61930–352monomerMolecule count 136.485 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12532[3]0.0098481726–0.34 1/AHas uncertainty columnMissing 085sasbdb/entries/k5/sasdwk5/source/SASDWK5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)5.6—Å
i0Guinier0.0615318—arbitrary
i0P(r)0.060432.6e-05arbitrary
mwExperimental36.1—kDa
mwPorod36.0—kDa
porod_volumePorod58.0—ų
rgGuinier2.180.002Å
rgP(r)2.090.001Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris, 200 mM NaClBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2024-08-04Storage / measurement temperature4.0 / 25.0
Exposure time0.5Frames620
Wavelength1.0Sample-to-detector distance—
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.098 – 3.4Sample volume / flow rate50.0 / 0.16

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDWK5
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure and analysis of a virulent chitinase from Listeria monocytogenes.
project.publication.titleStructure and analysis of a virulent chitinase from Listeria monocytogenes.
project.publication.author_listRehman S, Baczynska M, Zhang B, Lorenz CD, Garnett JA
project.publication.journalBiochim Biophys Acta Proteins Proteom
project.publication.doi10.1016/j.bbapap.2025.141106
project.publication.pmid41109581
project.publication.published_date2025 Oct 16
project.statusreleased
project.submitted_date2025-01-21
project.released_date2025-10-20
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experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namechitinase
experiment.sample.molecule[0].short_nameChiA
experiment.sample.molecule[0].sequenceMVATDDASVMPDISNKQVLVGYWHSWKSSGKDGYQQGTSADIALKDTPKAYNVVDVSFMKGDGVNRIPTFKPVGINDSDFRAQVGALNKEGRAVLLALGGADGHVELKAGDEEAFANEIIRQVETYGFDGLDIDLEQSAITAGDNKTVIPAALKIVKDHYKAEGKNFLITMAPEFPYLKPGSAYESYLTSLANYYDYIAPQLYNQGGDGVWVDETNQWIAQNNDTLKESFLYYMADSFINGTRGYLKIPANKFVFGLPANVDAAATGYVTDPQIVKNVFTRLQAKGTPVKGIMTWSVNWDAGKNKAGVPYNNSFSNAYGPIVGTKLEHHHHHH
experiment.sample.molecule[0].organismListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
experiment.sample.molecule[0].uniprot_codeQ8Y619
experiment.sample.molecule[0].uniprot_range_first30
experiment.sample.molecule[0].uniprot_range_last352
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNGKQVMVGGLSLLLVGAGLGAFGSTAQAATDDASVMPDISNKQVLVGYWHSWKSSGKDG YQQGTSADIALKDTPKAYNVVDVSFMKGDGVNRIPTFKPVGINDSDFRAQVGALNKEGRA VLLALGGADGHVELKAGDEEAFANEIIRQVETYGFDGLDIDLEQSAITAGDNKTVIPAAL KIVKDHYKAEGKNFLITMAPEFPYLKPGSAYESYLTSLANYYDYIAPQLYNQGGDGVWVD ETNQWIAQNNDTLKESFLYYMADSFINGTRGYLKIPANKFVFGLPANVDAAATGYVTDPQ IVKNVFTRLQAKGTPVKGIMTWSVNWDAGKNKAGVPYNNSFSNAYGPIVGTK
experiment.sample.molecule[0].mw36.485
experiment.sample.molecule[0].total_mw36.485
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris, 200 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameListeria monocytogenes Chitinase (ChiA)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressLondon, UK
experiment.contributor[0].affiliation[0].full_nameKing's College London
experiment.contributor[0].affiliation[0].webpagehttps://www.kcl.ac.uk
experiment.contributor[0].contributor_namejames
experiment.contributor[0].contributor_surnamegarnett
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-7621-8223
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-08-04
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames620
experiment.wavelength1.0
experiment.sample_detector_distancenull
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experiment.sample_volume50.0
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experiment.total_exposure_timenull
experiment.seccolumn19
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pddf_softwareATSAS GNOM
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experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw36.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw36.0
porod_mw_errornull
pddf_i00.06043
pddf_i0_error2.6e-05
guinier_i00.0615318
guinier_i0_errornull
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pddf_rg_error0.001
guinier_rg2.18
guinier_rg_error0.002
pddf_dmax5.6
pddf_dmax_errornull
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porod_volume_errornull
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estimated_volume_errornull
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guinier_point_last382
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
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symmetrynull
last_modified2025-10-20T08:59:40.863279+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDWK5
statussuccess
started_at2026-08-11T14:50:01.448980+00:00
finished_at2026-08-11T14:50:09.661283+00:00
source_last_modified2025-10-20T08:59:40.863279+02:00
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View the complete summary.json source
{
  "code": "SASDWK5",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure and analysis of a virulent chitinase from Listeria monocytogenes.",
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      "title": "Structure and analysis of a virulent chitinase from Listeria monocytogenes.",
      "author_list": "Rehman S, Baczynska M, Zhang B, Lorenz CD, Garnett JA",
      "journal": "Biochim Biophys Acta Proteins Proteom",
      "doi": "10.1016/j.bbapap.2025.141106",
      "pmid": "41109581",
      "published_date": "2025 Oct 16"
    },
    "status": "released",
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  },
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          "molecule_description": ""
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      "absorbption": null,
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View the complete manifest.json source
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