SASDWK9

Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-06-20T15:51:32.469391+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif

浓度— – 0.75 缓冲液 / pH50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol / 7.0
实验温度20.0 设备 / 束线CEITEC / Rigaku BioSAXS-1000
波长0.154 nm曝光600.0 s × 6

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488)
查看序列
GSHMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
proteinHomo sapiensO9579381–577other分子数 151.5 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1231[3]0.0100231–0.200061 1/A含误差列缺失 00sasbdb/entries/k9/sasdwk9/source/SASDWK9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)13.84—Å
i0Guinier0.284526—arbitrary
i0P(r)0.2814—arbitrary
mwExperimental85.65—kDa
mwPorod24.36—kDa
porod_volumePorod105.35—ų
rgGuinier5.077—Å
rgP(r)4.709—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · EOM/GAJOE ATSAS 2.8.4

χ²:0.893CorMap p:0.8745单位:1/A
模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1SASDWK9_12103eommixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
2SASDWK9_14329eommixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
3SASDWK9_statistic_fexibilityotherOther [static image] ———模型
4SASDWK9_15390mixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
5SASDWK9_164559eommixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
6SASDWK9_16774eommixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
7SASDWK9_17107eommixEOM/RANCH ATSAS 2.8.4—50.7—模型 图片 日志
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2020-04-05储存 / 测量温度4.0 / 20.0
曝光时间600.0帧数6
波长0.154样品-探测器距离0.48
光源X-ray in house探测器Pilatus 100K
机构 / 束线CEITEC / Rigaku BioSAXS-1000 · Brno, Czech Republic
q 范围0.1 – 2.001样品体积 / 流速100.0 / 0.6

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/k9/sasdwk9/source/SASDWK9.datdownloaded10678676931f9a3f12ab0e0f9b22877c38b6084d332e799bf19ffbffb79e05ec289d0下载查看原文件源站
full_entry_zipsasbdb/entries/k9/sasdwk9/source/SASDWK9.zipdownloaded25389717fc674cc7848ffa00d7fa378626bc2098717d3e1cdc1c468e79c27fb379e0fa下载查看原文件源站
pddfsasbdb/entries/k9/sasdwk9/source/SASDWK9.outdownloaded1996634ce420d7789d745a0fd9af08e0ebb87b2658b69f44d56deb30f1f28cb46b307下载查看原文件源站
sascifsasbdb/entries/k9/sasdwk9/source/SASDWK9.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/k9/sasdwk9/source/summary.jsondownloaded1217617ced256cf10caf83784b1cbbabc5857f093ea0ed4208e25cb4270c2f2bea335下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(14 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:234 个字段值
字段路径原始值
codeSASDWK9
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.author_listTripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.70669
project.publication.pmid42300088
project.publication.published_date2026 Jul
project.statusreleased
project.submitted_date2025-02-28
project.released_date2026-06-20
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWK9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWK9.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWK9_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWK9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWK9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWK9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWK9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 100K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameCEITEC
experiment.instrument.cityBrno
experiment.instrument.countryCzech Republic
experiment.instrument.beamline_nameRigaku BioSAXS-1000
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDouble-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488)
experiment.sample.molecule[0].short_namehStau1∆SSM
experiment.sample.molecule[0].sequenceGSHMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQF NGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESE EENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeO95793
experiment.sample.molecule[0].uniprot_range_first81
experiment.sample.molecule[0].uniprot_range_last577
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS ITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF PVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN LNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN AAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE KEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ PTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM VPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH VPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES CHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].mw51.5
experiment.sample.molecule[0].total_mw51.5
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionConstruct has truncated SSM motif - residues 454-488 in the Uniprot Sequence O95793 are deleted. The protein construct used for SAXS contains an additional three non-native amino acids at the N-terminus (GSH).
experiment.sample.buffer.name50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameCEITEC - Central European Institute of Technology, Masaryk University
experiment.contributor[0].affiliation[0].webpagehttps://www.ceitec.eu/
experiment.contributor[0].contributor_nameTomas
experiment.contributor[0].contributor_surnameKlumpler
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2020-04-05
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time600.0
experiment.number_of_frames6
experiment.wavelength0.154
experiment.sample_detector_distance0.48
experiment.concentration_minnull
experiment.concentration_max0.75
experiment.sample_volume100.0
experiment.flow_rate0.6
experiment.s_min0.1
experiment.s_max2.001
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[1].software_versionATSAS 2.8.4
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fits[0].models[2].model_plotnull
fits[0].models[2].softwareOther [static image]
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fits[0].models[2].type_of_modelother
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manifest.json:34 个字段值
字段路径原始值
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查看完整 summary.json 原文
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    "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
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      "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
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查看完整 manifest.json 原文
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