SASDWL7

D52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-11-23T16:42:15.833819+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · D52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)

浓度— – 12.9 缓冲液 / pH10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol / 7.4
实验温度10.0 设备 / 束线PETRA III / EMBL P12
波长0.123987 nm曝光0.995 s × 2400

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Iron-utilization periplasmic protein
查看序列
SDITVYNGQHKEAATAVAKAFEQETGIKVTLNSGKSEQLAGQLKEEGDKTPAAVFYTEQTATFADLSEAGLLAPISEQTIQQTAQKGVPLAPKKDWIALSGRSRVVVYDHTKLSEKDMEKSVLDYATPKWKGKIGYVSTSGAFLEQVVALSKMKGDKVALNWLKGLKENGKLYAKNSVALQAVENGEVPAALINNYYWYNLAKEKGVENLKSRLYFVRHQDPGALVSYSGAAVLKASKNQAEAQKFVDFLASKKGQEALVAARAEYPLRADVVSPFNLEPYEKLEAPVVSATTAQDKEHAIKLIEEAGLK
proteinHaemophilus influenzaeP3575523–332monomer分子数 133.768 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12600[3]0.0917866–7.3206 1/nm含误差列缺失 08sasbdb/entries/l7/sasdwl7/source/SASDWL7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.17—nm
i0Guinier16128.85.02arbitrary
i0P(r)16120.05.775arbitrary
mwExperimental31.6—kDa
mwPorod29.0—kDa
porod_volumePorod39.7—nm³
rgGuinier2.060.002nm
rgP(r)2.0490.001nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol缓冲液浓度—
pH7.4添加剂—
缓冲液说明PBS buffer
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2019-09-19储存 / 测量温度10.0 / 10.0
曝光时间0.995帧数2400
波长0.123987样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.092 – 7.321样品体积 / 流速75.0 / 0.6

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/l7/sasdwl7/source/SASDWL7.datdownloaded122918816e40168d284117a88f963e4344dc7bb7491f1cf882c394de2ebee7acc497c9下载查看原文件源站
full_entry_zipsasbdb/entries/l7/sasdwl7/source/SASDWL7.zipdownloaded983284a63cb406ca4b085d4de252e07e132c3b79fb6da19db998339cdbdfb441cc0fa下载查看原文件源站
pddfsasbdb/entries/l7/sasdwl7/source/SASDWL7.outdownloaded11775417568b455be1968314e4d2e1c680e454d5e6e56c32b0d47e6acdecca953ac617下载查看原文件源站
sascifsasbdb/entries/l7/sasdwl7/source/SASDWL7.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/l7/sasdwl7/source/summary.jsondownloaded7381c44749d3c7c489c1d928d5e6f3a51092cb346d5c24dc7e5cae6ee4e0a671cd3e下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:163 个字段值
字段路径原始值
codeSASDWL7
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleConformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations
project.publication.titleConformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations
project.publication.author_listLiu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z
project.publication.journalThe Journal of Chemical Physics
project.publication.doi10.1063/5.0136558
project.publication.pmidnull
project.publication.published_date2023 Feb 24
project.statusreleased
project.submitted_date2025-02-13
project.released_date2025-07-28
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIron-utilization periplasmic protein
experiment.sample.molecule[0].short_nameFBPA_HAEIN
experiment.sample.molecule[0].sequenceSDITVYNGQHKEAATAVAKAFEQETGIKVTLNSGKSEQLAGQLKEEGDKTPAAVFYTEQTATFADLSEAGLLAPISEQTIQQTAQKGVPLAPKKDWIALSGRSRVVVYDHTKLSEKDMEKSVLDYATPKWKGKIGYVSTSGAFLEQVVALSKMKGDKVALNWLKGLKENGKLYAKNSVALQAVENGEVPAALINNYYWYNLAKEKGVENLKSRLYFVRHQDPGALVSYSGAAVLKASKNQAEAQKFVDFLASKKGQEALVAARAEYPLRADVVSPFNLEPYEKLEAPVVSATTAQDKEHAIKLIEEAGLK
experiment.sample.molecule[0].organismHaemophilus influenzae
experiment.sample.molecule[0].uniprot_codeP35755
experiment.sample.molecule[0].uniprot_range_first23
experiment.sample.molecule[0].uniprot_range_last332
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMQFKHFKLATLAAALAFSANSFADITVYNGQHKEAATAVAKAFEQETGIKVTLNSGKSEQ LAGQLKEEGDKTPADVFYTEQTATFADLSEAGLLAPISEQTIQQTAQKGVPLAPKKDWIA LSGRSRVVVYDHTKLSEKDMEKSVLDYATPKWKGKIGYVSTSGAFLEQVVALSKMKGDKV ALNWLKGLKENGKLYAKNSVALQAVENGEVPAALINNYYWYNLAKEKGVENLKSRLYFVR HQDPGALVSYSGAAVLKASKNQAEAQKFVDFLASKKGQEALVAARAEYPLRADVVSPFNL EPYEKLEAPVVSATTAQDKEHAIKLIEEAGLK
experiment.sample.molecule[0].mw33.768
experiment.sample.molecule[0].total_mw33.768
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10 mM Na2HPO4 . 7H2O 1.8 mM KH2PO4 137 mM NaCl 2.7 mM KCl 5% v/v Glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentPBS buffer
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameD52A mutant of ferric binding protein (FbpA) in apo (no Fe) form in high ionic strength (HIS) buffer (PBS)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameSabanci University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameGoksin
experiment.contributor[0].contributor_surnameLiu
experiment.contributor[0].orcid
experiment.contributor[1].affiliation[0].short_namenull
experiment.contributor[1].affiliation[0].addressnull
experiment.contributor[1].affiliation[0].full_nameSabanci University
experiment.contributor[1].affiliation[0].webpagenull
experiment.contributor[1].contributor_nameZehra
experiment.contributor[1].contributor_surnameSayers
experiment.contributor[1].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-09-19
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.995
experiment.number_of_frames2400
experiment.wavelength0.123987
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max12.9
experiment.sample_volume75.0
experiment.flow_rate0.6
experiment.s_min0.092
experiment.s_max7.321
experiment.total_exposure_timenull
experiment.seccolumn1
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fits[0].models[0].bead_radius1.6
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fits[0].chi_square_value1.029
fits[0].p_value0.7378
fits[0].fit_residual_plotSASDWL7_fit1_fitresiduals_img.png
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw31.6
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw29.0
porod_mw_errornull
pddf_i016120.0
pddf_i0_error5.775
guinier_i016128.8
guinier_i0_error5.02
pddf_rg2.049
pddf_rg_error0.001
guinier_rg2.06
guinier_rg_error0.002
pddf_dmax6.17
pddf_dmax_errornull
porod_volume39.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last195
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWL7_datloglog_img.png
symmetrynull
last_modified2025-11-23T16:42:15.833819+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWL7
statussuccess
started_at2026-08-11T15:33:21.594773+00:00
finished_at2026-08-11T15:33:29.922942+00:00
source_last_modified2025-11-23T16:42:15.833819+01:00
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查看完整 summary.json 原文
{
  "code": "SASDWL7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations",
    "publication": {
      "title": "Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations",
      "author_list": "Liu G, Ekmen E, Jalalypour F, Mertens H, Jeffries C, Svergun D, Atilgan A, Atilgan C, Sayers Z",
      "journal": "The Journal of Chemical Physics",
      "doi": "10.1063/5.0136558",
      "pmid": null,
      "published_date": "2023 Feb 24"
    },
    "status": "released",
    "submitted_date": "2025-02-13",
    "released_date": "2025-07-28"
  },
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          "long_name": "Iron-utilization periplasmic protein",
          "short_name": "FBPA_HAEIN",
          "sequence": "SDITVYNGQHKEAATAVAKAFEQETGIKVTLNSGKSEQLAGQLKEEGDKTPAAVFYTEQTATFADLSEAGLLAPISEQTIQQTAQKGVPLAPKKDWIALSGRSRVVVYDHTKLSEKDMEKSVLDYATPKWKGKIGYVSTSGAFLEQVVALSKMKGDKVALNWLKGLKENGKLYAKNSVALQAVENGEVPAALINNYYWYNLAKEKGVENLKSRLYFVRHQDPGALVSYSGAAVLKASKNQAEAQKFVDFLASKKGQEALVAARAEYPLRADVVSPFNLEPYEKLEAPVVSATTAQDKEHAIKLIEEAGLK",
          "organism": "Haemophilus influenzae",
          "uniprot_code": "P35755",
          "uniprot_range_first": 23,
          "uniprot_range_last": 332,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MQFKHFKLATLAAALAFSANSFADITVYNGQHKEAATAVAKAFEQETGIKVTLNSGKSEQ\nLAGQLKEEGDKTPADVFYTEQTATFADLSEAGLLAPISEQTIQQTAQKGVPLAPKKDWIA\nLSGRSRVVVYDHTKLSEKDMEKSVLDYATPKWKGKIGYVSTSGAFLEQVVALSKMKGDKV\nALNWLKGLKENGKLYAKNSVALQAVENGEVPAALINNYYWYNLAKEKGVENLKSRLYFVR\nHQDPGALVSYSGAAVLKASKNQAEAQKFVDFLASKKGQEALVAARAEYPLRADVVSPFNL\nEPYEKLEAPVVSATTAQDKEHAIKLIEEAGLK",
          "mw": 33.768,
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          "number_molecules": 1,
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查看完整 manifest.json 原文
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