SASDWM3

Thomasclavelia ramosa immunoglobulin A protease N-terminal domain with middle (protease) domain

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-09-01T15:34:47.035373+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Thomasclavelia ramosa immunoglobulin A protease N-terminal domain with middle (protease) domain

浓度— – 1.14 缓冲液 / pH25 mM HEPES, 1 mM TCEP / 7.5
实验温度25.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline
波长0.11013 nm曝光1.0 s × 15

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
IgA protease
查看序列
ASKPDIKVGDYVKMGVYNNASILWRCVSIDNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEGKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDGDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAWPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPYIGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKTRSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASESTFDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNTIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHEFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKMLVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLETSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSDGSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQLKSGDTVAFQVLDENGNVLADDNTETQ
proteinThomasclavelia ramosaQ9AES231–807monomer分子数 188.017 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11992[3]0.00584215808–0.500809679 1/A含误差列缺失 0480sasbdb/entries/m3/sasdwm3/source/SASDWM3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.8—Å
i0Guinier0.0349861—arbitrary
i0P(r)0.03523—arbitrary
mwExperimental71.1—kDa
mwPorod67.0—kDa
porod_volumePorod107.7—ų
rgGuinier3.2910.224Å
rgP(r)3.35—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM HEPES, 1 mM TCEP缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-06-26储存 / 测量温度4.0 / 25.0
曝光时间1.0帧数15
波长0.11013样品-探测器距离—
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline · Ithaca, NY, USA
q 范围0.058 – 5.008样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/m3/sasdwm3/source/SASDWM3.datdownloaded96094ae378b8f558a960163d7ca84f57e148c1330ef3c13541439a18ad0893d33e230下载查看原文件源站
full_entry_zipsasbdb/entries/m3/sasdwm3/source/SASDWM3.zipdownloaded1918394ca84c33f2f3c3791d0a9661754c34b26355aeb1d47fbd6019c04f24eb80a65c下载查看原文件源站
pddfsasbdb/entries/m3/sasdwm3/source/SASDWM3.outdownloaded7867531b374e570413c8145e5165b9d09e3d966328ec3a7b869269c618c79fc898e67下载查看原文件源站
sascifsasbdb/entries/m3/sasdwm3/source/SASDWM3.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/m3/sasdwm3/source/summary.jsondownloaded871941e7298d68d1e6010bf99711687b88a37a17945577da4278ea976133c45c4069下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:159 个字段值
字段路径原始值
codeSASDWM3
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleStructure of the Thomasclavelia ramosa immunoglobulin A protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases.
project.publication.titleStructure of the Thomasclavelia ramosa immunoglobulin A protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases.
project.publication.author_listTran N, Frenette A, Holyoak T
project.publication.journalProc Natl Acad Sci U S A
project.publication.doi10.1073/pnas.2503549122
project.publication.pmid40854123
project.publication.published_date2025 Sep 2
project.statusreleased
project.submitted_date2024-12-06
project.released_date2025-08-11
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWM3.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWM3.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM3_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM3_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWM3_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM3_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWM3.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameID7A1 BioSAXS / HP-Bio Beamline
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIgA protease
experiment.sample.molecule[0].short_nameIgAP NTD+MD
experiment.sample.molecule[0].sequenceASKPDIKVGDYVKMGVYNNASILWRCVSIDNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEGKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDGDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAWPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPYIGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKTRSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASESTFDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNTIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHEFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKMLVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLETSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSDGSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQLKSGDTVAFQVLDENGNVLADDNTETQ
experiment.sample.molecule[0].organismThomasclavelia ramosa
experiment.sample.molecule[0].uniprot_codeQ9AES2
experiment.sample.molecule[0].uniprot_range_first31
experiment.sample.molecule[0].uniprot_range_last807
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTKKLMTKKITAIFLALYMAISVLPMTIQAASKPDIKVGDYVKMGVYNNASILWRCVSID NNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEG KVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDG DANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAW PYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPY IGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKT RSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASEST FDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNT IPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHE FGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKML VSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLE TSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSD GSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQ LKSGDTVAFQVLDENGNVLADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKL DLTPAKTLYDYEFIKVDGLNKPIVSDGTVVTYYYKNKNEEHTHNLTLVAAKAATCTTAGN SAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGTEWKSDDTNHWHECTVAGCGVI IESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTELKIIAGDNQIY NKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHTI TFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDK EATATKKGSKHEECKICGYKRSAVEIPATGTSTAPTDTTKPNDTTKPGNTNGSEKSPQTG DNSNIFLWFALLFVSAAGVTGITAYNKKKKEHAE
experiment.sample.molecule[0].mw88.017
experiment.sample.molecule[0].total_mw88.017
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionImmunoglobulin A protease N-terminal domain with middle domain (NTD+MD).
experiment.sample.buffer.name25 mM HEPES, 1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameThomasclavelia ramosa immunoglobulin A protease N-terminal domain with middle (protease) domain
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversity of Waterloo
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNorman
experiment.contributor[0].contributor_surnameTran
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-9372-9444
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-06-26
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time1.0
experiment.number_of_frames15
experiment.wavelength0.11013
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max1.14
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.058
experiment.s_max5.008
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDWM3_fit1_model1_img.png
fits[0].models[0].softwareCORAL
fits[0].models[0].pdb_link[0].pdb_codenone
fits[0].models[0].pdb_link[0].ext_codeQ9AES2
fits[0].models[0].pdb_link[0].ext_typealphafold
fits[0].models[0].pdb_link[0].difference_with_modelidentical
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelmix
fits[0].models[0].software_versionnull
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fits[0].models[0].model_mw88.01
fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user928
fits[0].fit_unit1/A
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fits[0].softwareCORAL
fits[0].chi_square_value0.996543
fits[0].p_value0.0002
fits[0].fit_residual_plotSASDWM3_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDWM3_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw71.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw67.0
porod_mw_errornull
pddf_i00.03523
pddf_i0_errornull
guinier_i00.0349861
guinier_i0_errornull
pddf_rg3.35
pddf_rg_errornull
guinier_rg3.291
guinier_rg_error0.224
pddf_dmax10.8
pddf_dmax_errornull
porod_volume107.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last123
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWM3_datloglog_img.png
symmetrynull
last_modified2025-09-01T15:34:47.035373+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWM3
statussuccess
started_at2026-08-11T14:03:48.602632+00:00
finished_at2026-08-11T14:03:56.757672+00:00
source_last_modified2025-09-01T15:34:47.035373+02:00
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDWM3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure of the Thomasclavelia ramosa immunoglobulin A protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases.",
    "publication": {
      "title": "Structure of the Thomasclavelia ramosa immunoglobulin A protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases.",
      "author_list": "Tran N, Frenette A, Holyoak T",
      "journal": "Proc Natl Acad Sci U S A",
      "doi": "10.1073/pnas.2503549122",
      "pmid": "40854123",
      "published_date": "2025 Sep 2"
    },
    "status": "released",
    "submitted_date": "2024-12-06",
    "released_date": "2025-08-11"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWM3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWM3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWM3_pofr_img.png",
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}
查看完整 manifest.json 原文
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