SASDWM9

Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-06-20T15:51:34.111789+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2

Concentration— – 10.4 Buffer / pH50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol / 8.0
Experimental temperature20.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.123983 nmExposure1.0 s × 45

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Double-stranded RNA-binding protein Staufen homolog 1 (178-577)
View sequence
GSHMEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
proteinHomo sapiensO95793178–577otherMolecule count 144.387 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11404[3]0.0923933–4.00073 1/nmHas uncertainty columnMissing 024sasbdb/entries/m9/sasdwm9/source/SASDWM9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.7—nm
i0Guinier2220.77—arbitrary
i0P(r)2198.0—arbitrary
mwExperimental44.7—kDa
mwPorod55.5—kDa
porod_volumePorod88.8—nm³
rgGuinier4.60.036nm
rgP(r)4.621—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanolBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2021-04-23Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames45
Wavelength0.123983Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 6M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.092 – 4.001Sample volume / flow rate100.0 / 0.7

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:169 field values
Field pathRaw value
codeSASDWM9
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.titleStaufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
project.publication.author_listTripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.70669
project.publication.pmid42300088
project.publication.published_date2026 Jul
project.statusreleased
project.submitted_date2025-02-28
project.released_date2026-06-20
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWM9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWM9.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWM9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWM9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWM9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDouble-stranded RNA-binding protein Staufen homolog 1 (178-577)
experiment.sample.molecule[0].short_namedsRBD3-End
experiment.sample.molecule[0].sequenceGSHMEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeO95793
experiment.sample.molecule[0].uniprot_range_first178
experiment.sample.molecule[0].uniprot_range_last577
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS ITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF PVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN LNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN AAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE KEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ PTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM VPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH VPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES CHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
experiment.sample.molecule[0].mw44.387
experiment.sample.molecule[0].total_mw44.387
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionHuman Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2. The protein construct used for SAXS contains an additional four non-native amino acids at the N-terminus (GSHM).
experiment.sample.buffer.name50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameCEITEC - Central European Institute of Technology, Masaryk University
experiment.contributor[0].affiliation[0].webpagehttps://www.ceitec.eu/
experiment.contributor[0].contributor_nameTomas
experiment.contributor[0].contributor_surnameKlumpler
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2021-04-23
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames45
experiment.wavelength0.123983
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max10.4
experiment.sample_volume100.0
experiment.flow_rate0.7
experiment.s_min0.092
experiment.s_max4.001
experiment.total_exposure_timenull
experiment.seccolumn1
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fits[0].fit_unit1/A
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fits[0].softwareEOM/GAJOE
fits[0].chi_square_value0.975
fits[0].p_value0.9339
fits[0].fit_residual_plotSASDWM9_fit1_fitresiduals_img.png
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fits[0].software_versionATSAS 2.8.4
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 in 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123983 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10.4 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 45 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw44.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw55.5
porod_mw_errornull
pddf_i02198.0
pddf_i0_errornull
guinier_i02220.77
guinier_i0_errornull
pddf_rg4.621
pddf_rg_errornull
guinier_rg4.6
guinier_rg_error0.036
pddf_dmax13.7
pddf_dmax_errornull
porod_volume88.8
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last69
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWM9_datloglog_img.png
symmetrynull
last_modified2026-06-20T15:51:34.111789+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDWM9
statussuccess
started_at2026-08-11T16:34:37.246768+00:00
finished_at2026-08-11T16:34:46.155142+00:00
source_last_modified2026-06-20T15:51:34.111789+02:00
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View the complete summary.json source
{
  "code": "SASDWM9",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
    "publication": {
      "title": "Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.",
      "author_list": "Tripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ",
      "journal": "Protein Sci",
      "doi": "10.1002/pro.70669",
      "pmid": "42300088",
      "published_date": "2026 Jul"
    },
    "status": "released",
    "submitted_date": "2025-02-28",
    "released_date": "2026-06-20"
  },
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      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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        {
          "long_name": "Double-stranded RNA-binding protein Staufen homolog 1 (178-577)",
          "short_name": "dsRBD3-End",
          "sequence": "GSHMEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC",
          "organism": "Homo sapiens",
          "uniprot_code": "O95793",
          "uniprot_range_first": 178,
          "uniprot_range_last": 577,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS\nITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF\nPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN\nLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN\nAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE\nKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ\nPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM\nVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH\nVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES\nCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC",
          "mw": 44.387,
          "total_mw": 44.387,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2. The protein construct used for SAXS contains an additional four non-native amino acids at the N-terminus (GSHM)."
        }
      ],
      "buffer": {
        "name": "50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "CEITEC - Central European Institute of Technology, Masaryk University",
            "webpage": "https://www.ceitec.eu/"
          }
        ],
        "contributor_name": "Tomas",
        "contributor_surname": "Klumpler",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-04-23",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 45,
    "wavelength": 0.123983,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 10.4,
    "sample_volume": 100.0,
    "flow_rate": 0.7,
    "s_min": 0.092,
    "s_max": 4.001,
    "total_exposure_time": null,
    "seccolumn": 1
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWM9_fit1_model1_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "ATSAS 2.8.4",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWM9_fit1_model1.pdb",
          "model_mw": 43.92,
          "bead_radius": 1.9,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_oeMwsLV.log",
          "symmetry": null,
          "comment": null,
          "user": 195
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWM9_fit1_model2_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "ATSAS 2.8.4",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWM9_fit1_model2.pdb",
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          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_X4ufjZg.log",
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        }
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWM9_fit1_fixed_fit_img.png",
      "software": "EOM/GAJOE",
      "chi_square_value": 0.975,
      "p_value": 0.9339,
      "fit_residual_plot": "SASDWM9_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDWM9_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_0nXrmoS.log",
      "software_version": "ATSAS 2.8.4",
      "description": ""
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  "i0_calibration_standard": null,
  "description": null,
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
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  "porod_mw_error": null,
  "pddf_i0": 2198.0,
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  "guinier_i0_error": null,
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  "guinier_rg": 4.6,
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  "pddf_dmax": 13.7,
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  "porod_volume": 88.8,
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  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 69,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDWM9_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-06-20T15:51:34.111789+02:00",
  "bragg_peak": []
}
View the complete manifest.json source
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