SASDWN2

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-03-06T17:20:11.215329+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4

浓度— – 11.0 缓冲液 / pH50 mM HEPES, 150 mM KCl / 7.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光2.0 s × 600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 - heavy chain
查看序列
EVQLQQSGPDLVKPGASVKISCKTSGYTFTEYIMHWVKQSHGKSLEWIGGIIPNNGGTSYNQKFKDKATMTVDKSSSTGYMELRSLTSEDSAVYYCTRREVYGRNYYALDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
proteinHomo sapiens——–—dimer分子数 249.525 kDa
Human immunoglobulin gamma 1 (IgG1) ChiLob 7/4 - kappa light chain
查看序列
DIQMTQTTSSLSASLGDRVTITCSASQGINNYLNWYQQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYSNLPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
proteinHomo sapiens——–—dimer分子数 223.444 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11000[3]0.0060067955–0.49585137 1/A含误差列缺失 013sasbdb/entries/n2/sasdwn2/source/SASDWN2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.0—Å
i0Guinier121.227—arbitrary
i0P(r)120.60.169arbitrary
mwExperimental130.891—kDa
mwPorod152.0—kDa
porod_volumePorod202.0—ų
rgGuinier4.9360.121Å
rgP(r)4.910.009Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES, 150 mM KCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-09-28储存 / 测量温度4.0 / 20.0
曝光时间2.0帧数600
波长0.099样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.06 – 4.959样品体积 / 流速45.0 / 0.25

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/n2/sasdwn2/source/SASDWN2.zipdownloaded3292912e5aa72a927803836907daa309c21e15446da9dfae12cd54516b7e0c5c397a7下载查看原文件源站
pddfsasbdb/entries/n2/sasdwn2/source/SASDWN2.outdownloaded56199d20c420d8edfa2d2f791efbe536ca9e51091b429e87d93a2467fdf90e4d1fa54下载查看原文件源站
sascifsasbdb/entries/n2/sasdwn2/source/SASDWN2.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/n2/sasdwn2/source/summary.jsondownloaded6630b18e1d7c3b46e034cf1a06fd4ca699348bdcd4ffd932ca5c312ec09cf1ed3a38下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDWN2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
project.publication.titleStructure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
project.publication.author_listElliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-025-58773-8
project.publication.pmid40221417
project.publication.published_date2025 Apr 12
project.statusreleased
project.submitted_date2023-07-12
project.released_date2025-02-25
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDWN2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDWN2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWN2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWN2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman immunoglobulin gamma 2 (IgG2) ChiLob 7/4 - heavy chain
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceEVQLQQSGPDLVKPGASVKISCKTSGYTFTEYIMHWVKQSHGKSLEWIGGIIPNNGGTSYNQKFKDKATMTVDKSSSTGYMELRSLTSEDSAVYYCTRREVYGRNYYALDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw49.525
experiment.sample.molecule[0].total_mw99.05
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameHuman immunoglobulin gamma 1 (IgG1) ChiLob 7/4 - kappa light chain
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceDIQMTQTTSSLSASLGDRVTITCSASQGINNYLNWYQQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYSNLPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequence
experiment.sample.molecule[1].mw23.444
experiment.sample.molecule[1].total_mw46.888
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name50 mM HEPES, 150 mM KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman immunoglobulin gamma 2 (IgG2) ChiLob 7/4
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressSouthampton, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Southampton
experiment.contributor[0].affiliation[0].webpagehttps://www.southampton.ac.uk/
experiment.contributor[0].contributor_nameIsabel
experiment.contributor[0].contributor_surnameElliott
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-4535-5932
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-09-28
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames600
experiment.wavelength0.099
experiment.sample_detector_distance2.7
experiment.concentration_minnull
experiment.concentration_max11.0
experiment.sample_volume45.0
experiment.flow_rate0.25
experiment.s_min0.06
experiment.s_max4.959
experiment.total_exposure_timenull
experiment.seccolumn10
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.0.1-1
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw130.891
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw152.0
porod_mw_errornull
pddf_i0120.6
pddf_i0_error0.169
guinier_i0121.227
guinier_i0_errornull
pddf_rg4.91
pddf_rg_error0.009
guinier_rg4.936
guinier_rg_error0.121
pddf_dmax16.0
pddf_dmax_errornull
porod_volume202.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last41
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWN2_datloglog_img.png
symmetrynull
last_modified2026-03-06T17:20:11.215329+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWN2
statussuccess
started_at2026-08-11T13:42:44.157828+00:00
finished_at2026-08-11T13:42:51.685129+00:00
source_last_modified2026-03-06T17:20:11.215329+01:00
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查看完整 summary.json 原文
{
  "code": "SASDWN2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
    "publication": {
      "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
      "author_list": "Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-58773-8",
      "pmid": "40221417",
      "published_date": "2025 Apr 12"
    },
    "status": "released",
    "submitted_date": "2023-07-12",
    "released_date": "2025-02-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWN2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWN2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWN2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWN2.sascif",
  "experiment": {
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      "detector": {
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        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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          "long_name": "Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 - heavy chain",
          "short_name": null,
          "sequence": "EVQLQQSGPDLVKPGASVKISCKTSGYTFTEYIMHWVKQSHGKSLEWIGGIIPNNGGTSYNQKFKDKATMTVDKSSSTGYMELRSLTSEDSAVYYCTRREVYGRNYYALDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK",
          "organism": "Homo sapiens",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 49.525,
          "total_mw": 99.05,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Human immunoglobulin gamma 1 (IgG1) ChiLob 7/4 - kappa light chain",
          "short_name": null,
          "sequence": "DIQMTQTTSSLSASLGDRVTITCSASQGINNYLNWYQQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYSNLPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC",
          "organism": "Homo sapiens",
          "uniprot_code": null,
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          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
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}
查看完整 manifest.json 原文
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