SASDWN4

Shewanella benthica hemoglobin 50 MPa

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-01-30T16:05:10.241401+01:00

50 MPa hydrostatic pressure.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Shewanella benthica hemoglobin 50 MPa

浓度— – 10.0 缓冲液 / pH14 mM Tris, 6 mM potassium phosphate / 7.0
实验温度23.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline
波长0.0883 nm曝光1.0 s × 4

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Group 1 truncated hemoglobin (C51S, C71S)
查看序列
SLYERLGGEQKIARIAADIFDTHATNPTVASRYKDSDRERVIKMVTEFLSAGTGGPQDYTGKSMPEAHRSMNINEAEYLAVIDDIMVALDKNEVGDQEKQELLMIAYSLKGEIIGA
proteinShewanella benthica KT99A9DF822–117other分子数 112.823 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11908[3]0.0114966775–0.580955482 1/A含误差列缺失 00sasbdb/entries/n4/sasdwn4/source/SASDWN4.dat
▼

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.0—Å
i0Guinier0.306475—arbitrary
mwExperimental30.24—kDa
porod_volumePorod70.0—ų
rgGuinier2.2130.151Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称14 mM Tris, 6 mM potassium phosphate缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-02-18储存 / 测量温度4.0 / 23.0
曝光时间1.0帧数4
波长0.0883样品-探测器距离1.782
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline · Ithaca, NY, USA
q 范围0.115 – 5.81样品体积 / 流速100.0 / 0.5

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/n4/sasdwn4/source/SASDWN4.outnot_listed——下载查看原文件
sascifsasbdb/entries/n4/sasdwn4/source/SASDWN4.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/n4/sasdwn4/source/summary.jsondownloaded55318d980df47f0bafe72d1647997d0f958018c905348460fe290b18457c0e8c03f2下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(1 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDWN4
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleExtremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N
project.publication.titleExtremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N
project.publication.author_listMartinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J
project.publication.journalJournal of Biological Chemistry
project.publication.doi10.1016/j.jbc.2025.108223
project.publication.pmidnull
project.publication.published_date2025 Jan
project.statusreleased
project.submitted_date2025-01-09
project.released_date2025-01-30
pddf_datanull
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intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWN4_kratky_img.png
pddf_plotnull
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDWN4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameID7A1 BioSAXS / HP-Bio Beamline
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGroup 1 truncated hemoglobin (C51S, C71S)
experiment.sample.molecule[0].short_nameS2SbHbN
experiment.sample.molecule[0].sequenceSLYERLGGEQKIARIAADIFDTHATNPTVASRYKDSDRERVIKMVTEFLSAGTGGPQDY TGKSMPEAHRSMNINEAEYLAVIDDIMVALDKNEVGDQEKQELLMIAYSLKGEIIGA
experiment.sample.molecule[0].organismShewanella benthica KT99
experiment.sample.molecule[0].uniprot_codeA9DF82
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last117
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSLYERLGGEQKIARIAADIFDTHATNPTVASRYKDSDRERVIKMVTEFLCAGTGGPQDY TGKSMPEAHRCMNINEAEYLAVIDDIMVALDKNEVGDQEKQELLMIAYSLKGEIIGA
experiment.sample.molecule[0].mw12.823
experiment.sample.molecule[0].total_mw12.823
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionShewanella benthica truncated hemoglobin N, S2SbHbN - UniProt A9DF82- with Met cleaved N-terminus and C51S, C71S point mutations.
experiment.sample.buffer.name14 mM Tris, 6 mM potassium phosphate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameShewanella benthica hemoglobin 50 MPa
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressIthaca, NY, United States
experiment.contributor[0].affiliation[0].full_nameCornell University
experiment.contributor[0].affiliation[0].webpagehttps://www.cornell.edu/
experiment.contributor[0].contributor_nameRichard
experiment.contributor[0].contributor_surnameGillilan
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-02-18
experiment.storage_temperature4.0
experiment.cell_temperature23.0
experiment.exposure_time1.0
experiment.number_of_frames4
experiment.wavelength0.0883
experiment.sample_detector_distance1.782
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volume100.0
experiment.flow_rate0.5
experiment.s_min0.115
experiment.s_max5.81
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
description50 MPa hydrostatic pressure.
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw30.24
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i00.306475
guinier_i0_errornull
pddf_rgnull
pddf_rg_errornull
guinier_rg2.213
guinier_rg_error0.151
pddf_dmax7.0
pddf_dmax_errornull
porod_volume70.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first44
guinier_point_last155
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWN4_datloglog_img.png
symmetrynull
last_modified2025-01-30T16:05:10.241401+01:00
bragg_peak[]
manifest.json:31 个字段值
字段路径原始值
codeSASDWN4
statussuccess
started_at2026-08-11T14:27:49.028281+00:00
finished_at2026-08-11T14:27:55.246558+00:00
source_last_modified2025-01-30T16:05:10.241401+01:00
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查看完整 summary.json 原文
{
  "code": "SASDWN4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N",
    "publication": {
      "title": "Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N",
      "author_list": "Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J",
      "journal": "Journal of Biological Chemistry",
      "doi": "10.1016/j.jbc.2025.108223",
      "pmid": null,
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    "status": "released",
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查看完整 manifest.json 原文
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