查看完整 summary.json 原文
{
"code": "SASDWP9",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "A Novel Biosensor for Ferrous Iron Developed via CoBiSe: A Computational Method for Rapid Biosensor Design",
"publication": {
"title": "A Novel Biosensor for Ferrous Iron Developed via CoBiSe: A Computational Method for Rapid Biosensor Design",
"author_list": "Papadopoulos A, Anlauf M, Reiners J, Paik S, Krüger A, Lückel B, Bott M, Drepper T, Frunzke J, Gohlke H, Weidtkamp-Peters S, Smits S, Gertzen C",
"journal": "ACS Sensors",
"doi": "10.1021/acssensors.5c02481",
"pmid": null,
"published_date": "2026 Jan 11"
},
"status": "released",
"submitted_date": "2025-02-26",
"released_date": "2025-12-11"
},
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"name": "Pilatus3 2M",
"resolution": 0.172
},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "point",
"type_of_source": "X-ray synchrotron",
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{
"long_name": "Ratiometric matryoshka biosensor for Fe2+",
"short_name": "MDtxRGA G149",
"sequence": "MRGSHHHHHHHHHHGMASMTGGQQMGRDLYDDDDKDPGRMKDLVDTTEMYLRTIYELEEEGIVPLRARIAERLEQSGPTVSQTVARMERDGLVHVSPDRSLEMTPEGRSLAIAVMRKHRLAERLLTDIIGLDIHKVHDEACRWEHVMSDEVERRLVEVLDDVHRSPFGNPIPGLGEIGLDQADEPDSGPASHNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFTWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMIFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGLEACEERMYPEDGALKSEYKEWLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDELYKGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNNPVRAIDLPLGENLKARIVQLNEILQVDLEQFQALTDAGVEIGTEVDIINEQGRVVITHNGSSVELIDDLAHAVRVEKVEG",
"organism": "synthetic construct",
"uniprot_code": null,
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"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "",
"mw": 84.189,
"total_mw": 84.189,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "The sensor contains different domains:\r\n\r\n- DtxR (ferrous iron binding protein) from Corynebacterium glutamicum\r\n- superfolder circularly permutated GFP (cpsfGFP (reporter fluorophore) from Aequorea victoria\r\n- LSSmApple (reference fluorophore) from Discosoma sp. \r\n\r\nand is constructed as:\r\n1-39 N-Terminal 10xHis, T7tag, enterokinase site/XpressTag, \r\n40-188 DtxR-N-terminus \r\n189-192 PASH Linker\r\n193-281 cpsfGFP-C-terminus\r\n282-284 GGT Linker\r\n285-519 LSSmApple\r\n520-522 GGS Linker\r\n523-666 cpsfGFP-N-terminus\r\n667-670 FNNP Linker\r\n671-749 DtxR-C-terminus"
}
],
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"name": "20 mM MOPS, 250 mM KCl,",
"concentration_unit": null,
"comment": null,
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"concentration": null,
"pka": null,
"ph": 7.0,
"deuteration": null
},
"purity_method": null,
"name": "Ratiometric matryoshka biosensor for Fe2+ – MDtxRGA_G149",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Düsseldorf, Germany",
"full_name": "Heinrich Heine University Düsseldorf",
"webpage": "https://www.uni-duesseldorf.de"
}
],
"contributor_name": "Jens",
"contributor_surname": "Reiners",
"orcid": ""
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2024-11-15",
"storage_temperature": 20.0,
"cell_temperature": 20.0,
"exposure_time": 2.0,
"number_of_frames": 1200,
"wavelength": 0.099,
"sample_detector_distance": 2.827,
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"flow_rate": 0.6,
"s_min": 0.085,
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"seccolumn": 1
},
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{
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"model_title": null,
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"software_version": "3",
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"symmetry": "P1",
"comment": null,
"user": 402
}
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"fit_unit": "1/A",
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"software": "CRYSOL",
"chi_square_value": 1.084,
"p_value": 0.3843,
"fit_residual_plot": "SASDWP9_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDWP9_fit1.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/fold_241017_af3_mdtxrga_g149_model_0.log",
"software_version": "Version 2.8.3",
"description": ""
}
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"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of Ratiometric matryoshka biosensor for Fe2+ (MDtxRGA_G149) in 20 mM MOPS, 250 mM KCl, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 4 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 1200 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "1/cm",
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"guinier_rg": 3.55,
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"pddf_dmax": 12.37,
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"porod_volume": 118.25,
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"estimated_volume": null,
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"guinier_point_first": 1,
"guinier_point_last": 58,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDWP9_datloglog_img.png",
"symmetry": null,
"last_modified": "2026-01-21T11:35:46.133284+01:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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