SASDWR3

BC120 G25C RNA

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-05-31T21:41:25.054885+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · BC120 G25C RNA

浓度— – 3.0 缓冲液 / pHphosphate buffered saline / 7.4
实验温度25.0 设备 / 束线Diamond Light Source / B21
波长0.094 nm曝光3.0 s × 600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
BC120 G25C RNA
查看序列
GGCCGGGCGCGGUGGCUCACGCCUCUAAUCCCAGCUCUCAGGGAGGCUAAGAGGCGGGAGGAUAGCUUGAGCCCAGGAGUUCGAGACCUGCCUGGGCAAUAUAGCGAGACCCCGUUCUUU
RNAHomo sapiens——–—monomer分子数 138.855 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12574[3]0.0045–0.34 1/A含误差列缺失 0101sasbdb/entries/r3/sasdwr3/source/SASDWR3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)13.83—Å
i0Guinier0.0575753—1/cm
i0P(r)0.05822—1/cm
mwExperimental45.43—kDa
mwPorod31.5—kDa
porod_volumePorod63.0—ų
rgGuinier4.0860.11Å
rgP(r)4.3—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称phosphate buffered saline缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-09-22储存 / 测量温度-80.0 / 25.0
曝光时间3.0帧数600
波长0.094样品-探测器距离3.7
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.045 – 3.4样品体积 / 流速60.0 / 0.16

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/r3/sasdwr3/source/SASDWR3.zipdownloaded1855814ffc6b76a3b9ac4972eb369e4714321269fba8572f2c1ddc99364653849a0718下载查看原文件源站
pddfsasbdb/entries/r3/sasdwr3/source/SASDWR3.outdownloaded199310096aefa5ffa8e069b1fe3bb3834eb1f17cc4a83a8dc0104c24736f96588573fd下载查看原文件源站
sascifsasbdb/entries/r3/sasdwr3/source/SASDWR3.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/r3/sasdwr3/source/summary.jsondownloaded89837f13cbc4c468a5bb05c857d18359900605257470b357ef37d8e093bc4ef4d806下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(8 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:202 个字段值
字段路径原始值
codeSASDWR3
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleAlu RNA pseudoknot alterations influence SRP9/SRP14 association.
project.publication.titleAlu RNA pseudoknot alterations influence SRP9/SRP14 association.
project.publication.author_listGussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA
project.publication.journalRNA
project.publication.doi10.1261/rna.080359.124
project.publication.pmid40345827
project.publication.published_date2025 May 9
project.statusreleased
project.submitted_date2024-05-24
project.released_date2025-05-31
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBC120 G25C RNA
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceGGCCGGGCGCGGUGGCUCACGCCUCUAAUCCCAGCUCUCAGGGAGGCUAAGAGGCGGGAGGAUAGCUUGAGCCCAGGAGUUCGAGACCUGCCUGGGCAAUAUAGCGAGACCCCGUUCUUU
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw38.855
experiment.sample.molecule[0].total_mw38.855
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.namephosphate buffered saline
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameBC120 G25C RNA
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressLethbridge, AB, Canada
experiment.contributor[0].affiliation[0].full_nameUniversity of Lethbridge
experiment.contributor[0].affiliation[0].webpagehttp://www.uleth.ca/
experiment.contributor[0].contributor_nameTrushar
experiment.contributor[0].contributor_surnamePatel
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-09-22
experiment.storage_temperature-80.0
experiment.cell_temperature25.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.094
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max3.0
experiment.sample_volume60.0
experiment.flow_rate0.16
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn19
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fits[2].software_versionnull
fits[2].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of BC120 G25C RNA in phosphate buffered saline, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 3 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 25°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw45.43
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw31.5
porod_mw_errornull
pddf_i00.05822
pddf_i0_errornull
guinier_i00.0575753
guinier_i0_errornull
pddf_rg4.3
pddf_rg_errornull
guinier_rg4.086
guinier_rg_error0.11
pddf_dmax13.83
pddf_dmax_errornull
porod_volume63.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first40
guinier_point_last198
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWR3_datloglog_img.png
symmetrynull
last_modified2025-05-31T21:41:25.054885+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDWR3
statussuccess
started_at2026-08-11T14:06:20.321350+00:00
finished_at2026-08-11T14:06:29.690197+00:00
source_last_modified2025-05-31T21:41:25.054885+02:00
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查看完整 summary.json 原文
{
  "code": "SASDWR3",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Alu RNA pseudoknot alterations influence SRP9/SRP14 association.",
    "publication": {
      "title": "Alu RNA pseudoknot alterations influence SRP9/SRP14 association.",
      "author_list": "Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA",
      "journal": "RNA",
      "doi": "10.1261/rna.080359.124",
      "pmid": "40345827",
      "published_date": "2025 May 9"
    },
    "status": "released",
    "submitted_date": "2024-05-24",
    "released_date": "2025-05-31"
  },
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    "sample": {
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查看完整 manifest.json 原文
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