SASDWW2

Human immunoglobulin gamma 2 (IgG2) SAP1.3

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-03-06T17:19:20.139073+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human immunoglobulin gamma 2 (IgG2) SAP1.3

Concentration— – 7.5 Buffer / pH50 mM HEPES, 150 mM KCl / 7.5
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.099 nmExposure2.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Human immunoglobulin SAP1.3 - kappa chain
View sequence
DIVMTQAAFSNPVTLGTSASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLASGVPDRFSSSGSGTDFTLRISRVEAEDVGVYYCAQNLELPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
proteinHomo sapiens——–—dimerMolecule count 223.908 kDa
Human immunoglobulin gamma 2 (IgG2) SAP1.3 - heavy chain
View sequence
QVQLKQSGPGLVQPSQTLSITCTVSGFSLTSYGVHWVRQSPGKGLEWLGVIWSGGITDYNAAFISRLSISKDNSKSQVFFKVNSLQPNDTAIYYCARNGRIGSTMTLYYAMDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
proteinHomo sapiens——–—dimerMolecule count 249.436 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11000[3]0.0060067955–0.49585137 1/AHas uncertainty columnMissing 015sasbdb/entries/w2/sasdww2/source/SASDWW2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.9—Å
i0Guinier192.232—arbitrary
i0P(r)192.00.164arbitrary
mwExperimental157.1—kDa
mwPorod152.0—kDa
porod_volumePorod214.0—ų
rgGuinier4.8890.071Å
rgP(r)4.90.005Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM HEPES, 150 mM KClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2024-09-28Storage / measurement temperature3.7 / 20.0
Exposure time2.0Frames600
Wavelength0.099Sample-to-detector distance2.7
SourceX-ray synchrotronDetectorPilatus3 2M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.06 – 4.959Sample volume / flow rate35.0 / 0.25

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDWW2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
project.publication.titleStructure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.
project.publication.author_listElliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-025-58773-8
project.publication.pmid40221417
project.publication.published_date2025 Apr 12
project.statusreleased
project.submitted_date2023-07-12
project.released_date2025-02-25
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman immunoglobulin SAP1.3 - kappa chain
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceDIVMTQAAFSNPVTLGTSASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLASGVPDRFSSSGSGTDFTLRISRVEAEDVGVYYCAQNLELPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw23.908
experiment.sample.molecule[0].total_mw47.816
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.molecule[1].long_nameHuman immunoglobulin gamma 2 (IgG2) SAP1.3 - heavy chain
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceQVQLKQSGPGLVQPSQTLSITCTVSGFSLTSYGVHWVRQSPGKGLEWLGVIWSGGITDYNAAFISRLSISKDNSKSQVFFKVNSLQPNDTAIYYCARNGRIGSTMTLYYAMDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequence
experiment.sample.molecule[1].mw49.436
experiment.sample.molecule[1].total_mw98.872
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name50 mM HEPES, 150 mM KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman immunoglobulin gamma 2 (IgG2) SAP1.3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressSouthampton, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Southampton
experiment.contributor[0].affiliation[0].webpagehttps://www.southampton.ac.uk/
experiment.contributor[0].contributor_nameIsabel
experiment.contributor[0].contributor_surnameElliott
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-4535-5932
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-09-28
experiment.storage_temperature3.7
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames600
experiment.wavelength0.099
experiment.sample_detector_distance2.7
experiment.concentration_minnull
experiment.concentration_max7.5
experiment.sample_volume35.0
experiment.flow_rate0.25
experiment.s_min0.06
experiment.s_max4.959
experiment.total_exposure_timenull
experiment.seccolumn10
fits[]
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pddf_softwareATSAS GNOM
pddf_software_version4.0.1-1
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw157.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw152.0
porod_mw_errornull
pddf_i0192.0
pddf_i0_error0.164
guinier_i0192.232
guinier_i0_errornull
pddf_rg4.9
pddf_rg_error0.005
guinier_rg4.889
guinier_rg_error0.071
pddf_dmax15.9
pddf_dmax_errornull
porod_volume214.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last42
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDWW2_datloglog_img.png
symmetrynull
last_modified2026-03-06T17:19:20.139073+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDWW2
statussuccess
started_at2026-08-11T13:48:11.311746+00:00
finished_at2026-08-11T13:48:19.627749+00:00
source_last_modified2026-03-06T17:19:20.139073+01:00
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View the complete summary.json source
{
  "code": "SASDWW2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
    "publication": {
      "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
      "author_list": "Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-58773-8",
      "pmid": "40221417",
      "published_date": "2025 Apr 12"
    },
    "status": "released",
    "submitted_date": "2023-07-12",
    "released_date": "2025-02-25"
  },
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        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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          "short_name": null,
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        "ph": 7.5,
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      },
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      "name": "Human immunoglobulin gamma 2 (IgG2) SAP1.3",
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    },
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View the complete manifest.json source
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