SASDX22

chloroplast trigger factor from Chlamydomonas reinhardtii

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2025-03-07T08:15:48.467610+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · chloroplast trigger factor from Chlamydomonas reinhardtii

Concentration0.5 – 8.0 Buffer / pH20 mM Tris pH 7.5, 150 mM KCl / —
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.09919 nmExposure1.0 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
peptidylprolyl isomerase
View sequence
AAAGKEKDVKVEVSVAVEELGTCSRSAIITVPASTVKDIFKRGVKRIERDVVGQLKGWQAGKPLPLSMVIQQVGGQNKFKTFCLEELLLEALPKGIESARNGRAVDPASVTVPPEDFPSMLERYDPAKEFFFRAMFDVAPPVVWRTPLEELEVTIRDTGDFSTDAAAADDLIRQYRKQHGFSRVVAGRGLQLGDTLVIDLEITSKATGQALPGLTHKRFSFDTEADVLGITSGMLGMKAGESRTFNMSMPEDYDVEFWQSMPVKVAAKVHEIFEWTLPEFNDEYVAKQHEGKWGSAKEMREALIASTAMQRVTELDKALEDAVVKAVADALDMPEVPPRMVEQLGERQFQAQLLQMIEDRIGSREDVEKLATEEMAAEFIRERKKDLEDQVKFNLAVDDIWVRKGLVLEDEAVEAEFSLRARQMEAVGQPFDREDMLDDVRETVKSVTVIEWLKDNVKRHVLPYTAADDKAAKAKGEPVAV
proteinChlamydomonas reinhardtiiA8JD5668–548monomerMolecule count 153.731 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11043[3]0.03479421–4.943923 1/nmHas uncertainty columnMissing 02sasbdb/entries/22/sasdx22/source/SASDX22.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.46—nm
i0Guinier51.8001—arbitrary
i0P(r)50.96—arbitrary
mwExperimental49.0—kDa
mwPorod61.0—kDa
porod_volumePorod103.37—nm³
rgGuinier3.850.016nm
rgP(r)3.851—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris pH 7.5, 150 mM KClBuffer concentration—
pH—Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-04-30Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames10
Wavelength0.09919Sample-to-detector distance2.867
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.035 – 4.944Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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sascifsasbdb/entries/22/sasdx22/source/SASDX22.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/22/sasdx22/source/summary.jsondownloaded8046301686f39e956628571bef68dca999a46bafb4f61ce194b3e768d050bca99877DownloadView source fileSource site
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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDX22
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructural and molecular comparison of bacterial and eukaryotic trigger factors.
project.publication.titleStructural and molecular comparison of bacterial and eukaryotic trigger factors.
project.publication.author_listRies F, Carius Y, Rohr M, Gries K, Keller S, Lancaster CRD, Willmund F
project.publication.journalSci Rep
project.publication.doi10.1038/s41598-017-10625-2
project.publication.pmid28878399
project.publication.published_date2017 Sep 6
project.statusreleased
project.submitted_date2025-02-27
project.released_date2025-03-07
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDX22.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDX22.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX22_dat_img.png
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX22_guinier_img.png
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namepeptidylprolyl isomerase
experiment.sample.molecule[0].short_nameCrTIG1
experiment.sample.molecule[0].sequenceAAAGKEKDVKVEVSVAVEELGTCSRSAIITVPASTVKDIFKRGVKRIERDVVG QLKGWQAGKPLPLSMVIQQVGGQNKFKTFCLEELLLEALPKGIESARNGRAVDPASVTVP PEDFPSMLERYDPAKEFFFRAMFDVAPPVVWRTPLEELEVTIRDTGDFSTDAAAADDLIR QYRKQHGFSRVVAGRGLQLGDTLVIDLEITSKATGQALPGLTHKRFSFDTEADVLGITSG MLGMKAGESRTFNMSMPEDYDVEFWQSMPVKVAAKVHEIFEWTLPEFNDEYVAKQHEGKW GSAKEMREALIASTAMQRVTELDKALEDAVVKAVADALDMPEVPPRMVEQLGERQFQAQL LQMIEDRIGSREDVEKLATEEMAAEFIRERKKDLEDQVKFNLAVDDIWVRKGLVLEDEAV EAEFSLRARQMEAVGQPFDREDMLDDVRETVKSVTVIEWLKDNVKRHVLPYTAADDKAAK AKGEPVAV
experiment.sample.molecule[0].organismChlamydomonas reinhardtii
experiment.sample.molecule[0].uniprot_codeA8JD56
experiment.sample.molecule[0].uniprot_range_first68
experiment.sample.molecule[0].uniprot_range_last548
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMMLTLKNQVLAARAGKRTACAPAGLPRSSGATQALVAAVGAPALAAPTATPCARRSVDVS RGALQVCAAAGKEKDVKVEVSVAVEELGTCSRSAIITVPASTVKDIFKRGVKRIERDVVG QLKGWQAGKPLPLSMVIQQVGGQNKFKTFCLEELLLEALPKGIESARNGRAVDPASVTVP PEDFPSMLERYDPAKEFFFRAMFDVAPPVVWRTPLEELEVTIRDTGDFSTDAAAADDLIR QYRKQHGFSRVVAGRGLQLGDTLVIDLEITSKATGQALPGLTHKRFSFDTEADVLGITSG MLGMKAGESRTFNMSMPEDYDVEFWQSMPVKVAAKVHEIFEWTLPEFNDEYVAKQHEGKW GSAKEMREALIASTAMQRVTELDKALEDAVVKAVADALDMPEVPPRMVEQLGERQFQAQL LQMIEDRIGSREDVEKLATEEMAAEFIRERKKDLEDQVKFNLAVDDIWVRKGLVLEDEAV EAEFSLRARQMEAVGQPFDREDMLDDVRETVKSVTVIEWLKDNVKRHVLPYTAADDKAAK AKGEPVAV
experiment.sample.molecule[0].mw53.731
experiment.sample.molecule[0].total_mw53.731
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris pH 7.5, 150 mM KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.phnull
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namechloroplast trigger factor from Chlamydomonas reinhardtii
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameSaarland University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameYvonne
experiment.contributor[0].contributor_surnameCarius
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-3902-3122
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-04-30
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames10
experiment.wavelength0.09919
experiment.sample_detector_distance2.867
experiment.concentration_min0.5
experiment.concentration_max8.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.035
experiment.s_max4.944
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
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fits[0].models[0].model_mw79.25
fits[0].models[0].bead_radius3.75
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1190
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fits[0].softwareDAMMIN
fits[0].chi_square_value0.792
fits[0].p_value0.0
fits[0].fit_residual_plotSASDX22_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of chloroplast trigger factor from Chlamydomonas reinhardtii in 20 mM Tris pH 7.5, 150 mM KCl were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 8 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unitarbitrary
experimental_mw49.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw61.0
porod_mw_errornull
pddf_i050.96
pddf_i0_errornull
guinier_i051.8001
guinier_i0_errornull
pddf_rg3.851
pddf_rg_errornull
guinier_rg3.85
guinier_rg_error0.016
pddf_dmax12.46
pddf_dmax_errornull
porod_volume103.37
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first32
guinier_point_last63
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDX22_datloglog_img.png
symmetrynull
last_modified2025-03-07T08:15:48.467610+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDX22
statussuccess
started_at2026-08-11T13:29:15.974653+00:00
finished_at2026-08-11T13:29:23.628668+00:00
source_last_modified2025-03-07T08:15:48.467610+01:00
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View the complete summary.json source
{
  "code": "SASDX22",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural and molecular comparison of bacterial and eukaryotic trigger factors.",
    "publication": {
      "title": "Structural and molecular comparison of bacterial and eukaryotic trigger factors.",
      "author_list": "Ries F, Carius Y, Rohr M, Gries K, Keller S, Lancaster CRD, Willmund F",
      "journal": "Sci Rep",
      "doi": "10.1038/s41598-017-10625-2",
      "pmid": "28878399",
      "published_date": "2017 Sep 6"
    },
    "status": "released",
    "submitted_date": "2025-02-27",
    "released_date": "2025-03-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDX22.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDX22.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX22_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX22_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX22_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX22_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDX22.sascif",
  "experiment": {
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        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "peptidylprolyl isomerase",
          "short_name": "CrTIG1",
          "sequence": "AAAGKEKDVKVEVSVAVEELGTCSRSAIITVPASTVKDIFKRGVKRIERDVVG\r\nQLKGWQAGKPLPLSMVIQQVGGQNKFKTFCLEELLLEALPKGIESARNGRAVDPASVTVP\r\nPEDFPSMLERYDPAKEFFFRAMFDVAPPVVWRTPLEELEVTIRDTGDFSTDAAAADDLIR\r\nQYRKQHGFSRVVAGRGLQLGDTLVIDLEITSKATGQALPGLTHKRFSFDTEADVLGITSG\r\nMLGMKAGESRTFNMSMPEDYDVEFWQSMPVKVAAKVHEIFEWTLPEFNDEYVAKQHEGKW\r\nGSAKEMREALIASTAMQRVTELDKALEDAVVKAVADALDMPEVPPRMVEQLGERQFQAQL\r\nLQMIEDRIGSREDVEKLATEEMAAEFIRERKKDLEDQVKFNLAVDDIWVRKGLVLEDEAV\r\nEAEFSLRARQMEAVGQPFDREDMLDDVRETVKSVTVIEWLKDNVKRHVLPYTAADDKAAK\r\nAKGEPVAV",
          "organism": "Chlamydomonas reinhardtii",
          "uniprot_code": "A8JD56",
          "uniprot_range_first": 68,
          "uniprot_range_last": 548,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MMLTLKNQVLAARAGKRTACAPAGLPRSSGATQALVAAVGAPALAAPTATPCARRSVDVS\nRGALQVCAAAGKEKDVKVEVSVAVEELGTCSRSAIITVPASTVKDIFKRGVKRIERDVVG\nQLKGWQAGKPLPLSMVIQQVGGQNKFKTFCLEELLLEALPKGIESARNGRAVDPASVTVP\nPEDFPSMLERYDPAKEFFFRAMFDVAPPVVWRTPLEELEVTIRDTGDFSTDAAAADDLIR\nQYRKQHGFSRVVAGRGLQLGDTLVIDLEITSKATGQALPGLTHKRFSFDTEADVLGITSG\nMLGMKAGESRTFNMSMPEDYDVEFWQSMPVKVAAKVHEIFEWTLPEFNDEYVAKQHEGKW\nGSAKEMREALIASTAMQRVTELDKALEDAVVKAVADALDMPEVPPRMVEQLGERQFQAQL\nLQMIEDRIGSREDVEKLATEEMAAEFIRERKKDLEDQVKFNLAVDDIWVRKGLVLEDEAV\nEAEFSLRARQMEAVGQPFDREDMLDDVRETVKSVTVIEWLKDNVKRHVLPYTAADDKAAK\nAKGEPVAV",
          "mw": 53.731,
          "total_mw": 53.731,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
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View the complete manifest.json source
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