Non Structural Protein 15 and ADPRP Macrodomain D

{
  "code": "SASDX29",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Non Structural Protein 15 in Complex with ADPRP Macrodomain of SARS CoV 2",
    "publication": null,
    "status": "released",
    "submitted_date": "2025-08-24",
    "released_date": "2025-09-17"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDX29.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX29_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX29_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX29_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX29_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDX29.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Hybrid Photon Counting (HPC)",
        "name": "Mythen2 R 1K",
        "resolution": null
      },
      "name": "CSIR-Central Drug Research Institute",
      "city": "Lucknow",
      "country": "India",
      "beamline_name": "Anton Paar SAXSpace",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Non structural Protein 15 in Complex with ADPRP Macrodomain",
          "short_name": "NSP15",
          "sequence": "MTIFAPQHKADFGIIDGHFNNFANNAQILAFNDFHKIIQQQINNAANTFAATGLCIGDKIVFHGAIHRNEILKFKRAFFKTFGQTNQQMQTTLAMAKITIHDMFKGIAFQFITFDKFIHGQFQKINFHLATRFKFLQITALGKIGFRQLLNNAIDFFVIVELGFNCFTNQGNTIQARLFRTNARLQTFNATFGNQNTITCITKQVNLTIHTAIKKNGQRCTNGFGRFFCNIGHATNTNGANMCRCITFIIPDNGIGCNINTQIIQNFHFRNRFDITFCPQFKRNINRQCGFILKQFNINTIHFGIHGIVNNANRNFTLLTIKMAFVHNIKGNIFQAGSMRPR\r\nMEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEKLLGLMGLPLTARFPVGKPVVP",
          "organism": "Severe acute respiratory syndrome coronavirus 2",
          "uniprot_code": "P0DTD1",
          "uniprot_range_first": 6465,
          "uniprot_range_last": 6798,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": 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          "mw": 254.0,
          "total_mw": 254.0,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "SAXS data were collected on SARS-CoV-2 NSP15 in solution. The protein forms a hexamer with a theoretical molecular weight of approximately 234 kDa (6 × 39.0 kDa), consistent with the oligomeric state observed under our assay conditions. In the presence of the ADPRP (Mac1) macrodomain, the SAXS profile corresponds to a hexameric NSP15 bound to a single ADPRP macrodomain, giving an expected molecular weight of approximately 253 kDa (NSP15 hexamer ~234 kDa plus ADPRP ~19 kDa), which agrees well with the SAXS-derived molecular weight estimates."
        }
      ],
      "buffer": {
        "name": "20mM HEPES, 150mM Nacl",
        "concentration_unit": null,
        "comment": "SAXS data collected to study the solution conformation and oligomeric state of SARS-CoV-2 NSP15 unde",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Non Structural Protein 15 and ADPRP Macrodomain D",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "India",
            "full_name": "CSIR - Central Drug Research Institute",
            "webpage": "https://cdri.res.in/"
          }
        ],
        "contributor_name": "Hira Singh",
        "contributor_surname": "Gariya",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-03-20",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 900.0,
    "number_of_frames": 3,
    "wavelength": 1.54,
    "sample_detector_distance": 0.317,
    "concentration_min": null,
    "concentration_max": 3.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.116,
    "s_max": 7.78,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 253.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": 208.0,
  "guinier_i0_mw_error": null,
  "porod_mw": 142.0,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
  "guinier_i0": 23824.4,
  "guinier_i0_error": null,
  "pddf_rg": 0.354,
  "pddf_rg_error": null,
  "guinier_rg": 6.091,
  "guinier_rg_error": 0.276,
  "pddf_dmax": null,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 83,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDX29_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-09-17T14:40:15.243339+02:00",
  "bragg_peak": []
}

{
  "code": "SASDX29",
  "status": "success",
  "started_at": "2026-08-11T16:04:51.679065+00:00",
  "finished_at": "2026-08-11T16:04:57.374587+00:00",
  "source_last_modified": "2025-09-17T14:40:15.243339+02:00",
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