SASDX42

IgA peptidase (A31-I1166; active site mutant E540A)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-07-21T18:12:24.155654+02:00

The results obtained from EOM analysis are made available in the full entry zip archive.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · IgA peptidase (A31-I1166; active site mutant E540A)

浓度— – 4.0 缓冲液 / pH50 mM Tris, 150 mM NaCl / 8.0
实验温度20.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光4.0 s × 250

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
IgA protease (E540A)
查看序列
MASKPDIKVGDYVKMGVYNNASILWRCVSIDNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEGKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDGDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAWPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPYIGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKTRSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASESTFDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNTIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHAFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKMLVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLETSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSDGSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQLKSGDTVAFQVLDENGNVLADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKLDLTPAKTLYDYEFIKVDGLNKPIVSDGTVVTYYYKNKNEEHTHNLTLVAAKAATCTTAGNSAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGTEWKSDDTNHWHECTVAGCGVIIESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTELKIIAGDNQIYNKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHTITFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDKEATATKKGSKHEECKICGYKRSAVEILEHHHHHH
proteinThomasclavelia ramosaQ9AES231–1166monomer分子数 1127.857 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11000[3]0.06285053–5.209716 1/nm含误差列缺失 016sasbdb/entries/42/sasdx42/source/SASDX42.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)27.9—nm
i0Guinier70.169—arbitrary
i0P(r)72.97—arbitrary
mwExperimental118.8—kDa
mwPorod119.0—kDa
porod_volumePorod190.0—nm³
rgGuinier5.650.035nm
rgP(r)6.433—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris, 150 mM NaCl缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-05-23储存 / 测量温度4.0 / 20.0
曝光时间4.0帧数250
波长0.099样品-探测器距离2.867
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.063 – 5.21样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/42/sasdx42/source/SASDX42.datdownloaded47210ceabada7822f4d26ce9e24042810d4abcdd405d9761248b948499d36f3564441下载查看原文件源站
full_entry_zipsasbdb/entries/42/sasdx42/source/SASDX42.zipdownloaded1656403b32a8840f5c57b0c830d54a7b467431ed352e02f4f532f6a6ac2154bacb25a7f下载查看原文件源站
pddfsasbdb/entries/42/sasdx42/source/SASDX42.outdownloaded479905159354e8b0983717278d4cafc2c644bf9177eb98fc021a80265b38507493792下载查看原文件源站
sascifsasbdb/entries/42/sasdx42/source/SASDX42.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/42/sasdx42/source/summary.jsondownloaded1191468cea969ccc2a8e1133cf44daecde3dcc782066fda8beeb75c7ac6ca6d9675d2下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(11 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:208 个字段值
字段路径原始值
codeSASDX42
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleBiochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.
project.publication.titleBiochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.
project.publication.author_listRamírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1013292
project.publication.pmid40627637
project.publication.published_date2025 Jul
project.statusreleased
project.submitted_date2025-02-27
project.released_date2025-06-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDX42.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDX42.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX42_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX42_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDX42.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIgA protease (E540A)
experiment.sample.molecule[0].short_nameIgAse
experiment.sample.molecule[0].sequenceMASKPDIKVGDYVKMGVYNNASILWRCVSIDNNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDY GSNYWKDSNMRSWLNSTAAEGKVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVS HPEYNKGIVDGDANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMA WPYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPYIGSAPNKQE DDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKTRSDTENMVVVVCGEGYTKS QQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASESTFDNGGSTFFDVIVDKYNSPVISNNLHGSQ WKNHIFERCIGPEFIEKIHDAHIKKKCDPNTIPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREY GFHYFISPSDSYRASKTFAHAFGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTY TCRNAYGSKMLVSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDL ETSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSDGSVATDSSG NPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQLKSGDTVAFQVLDENGNVL ADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKLDLTPAKTLYDYEFIKVDGLNKPIVSDGTV VTYYYKNKNEEHTHNLTLVAAKAATCTTAGNSAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGT EWKSDDTNHWHECTVAGCGVIIESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTE LKIIAGDNQIYNKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHT ITFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDKEATATKKGS KHEECKICGYKRSAVEILEHHHHHH
experiment.sample.molecule[0].organismThomasclavelia ramosa
experiment.sample.molecule[0].uniprot_codeQ9AES2
experiment.sample.molecule[0].uniprot_range_first31
experiment.sample.molecule[0].uniprot_range_last1166
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTKKLMTKKITAIFLALYMAISVLPMTIQAASKPDIKVGDYVKMGVYNNASILWRCVSID NNGPLMLADKIVDTLAYDAKTNDNSNSKSHSRSYKRDDYGSNYWKDSNMRSWLNSTAAEG KVDWLCGNPPKDGYVSGVGAYNEKAGFLNAFSKSEIAAMKTVTQRSLVSHPEYNKGIVDG DANSDLLYYTDISEAVANYDSSYFETTTEKVFLLDVKQANAVWKNLKGYYVAYNNDGMAW PYWLRTPVTDCNHDMRYISSSGQVGRYAPWYSDLGVRPAFYLDSEYFVTTSGSGSQSSPY IGSAPNKQEDDYTISEPAEDANPDWNVSTEQSIQLTLGPWYSNDGKYSNPTIPVYTIQKT RSDTENMVVVVCGEGYTKSQQGKFINDVKRLWQDAMKYEPYRSYADRFNVYALCTASEST FDNGGSTFFDVIVDKYNSPVISNNLHGSQWKNHIFERCIGPEFIEKIHDAHIKKKCDPNT IPSGSEYEPYYYVHDYIAQFAMVVNTKSDFGGAYNNREYGFHYFISPSDSYRASKTFAHE FGHGLLGLGDEYSNGYLLDDKELKSLNLSSVEDPEKIKWRQLLGFRNTYTCRNAYGSKML VSSYECIMRDTNYQFCEVCRLQGFKRMSQLVKDVDLYVATPEVKEYTGAYSKPSDFTDLE TSSYYNYTYNRNDRLLSGNSKSRFNTNMNGKKIELRTVIQNISDKNARQLKFKMWIKHSD GSVATDSSGNPLQTVQTFDIPVWNDKANFWPLGALDHIKSDFNSGLKSCSLIYQIPSDAQ LKSGDTVAFQVLDENGNVLADDNTETQRYTTVSIQYKFEDGSEIPNTAGGTFTVPYGTKL DLTPAKTLYDYEFIKVDGLNKPIVSDGTVVTYYYKNKNEEHTHNLTLVAAKAATCTTAGN SAYYTCDGCDKWFADATGSVEITDKTSVKIPAPGHTAGTEWKSDDTNHWHECTVAGCGVI IESTKSAHTAGEWIVDTPATATTAGTKHKECTVCHRVLETQPIPSTGTELKIIAGDNQIY NKASGSDVTITCNGDFAKFTGIKVDGSVVDSSNYTAVSGSTVLTLKASYLGTLTDGSHTI TFVYTDGEANANLTVRTAGSGHIHDYGTEWKSNADNHWHECNCGDKKDEAAHSFKWVVDK EATATKKGSKHEECKICGYKRSAVEIPATGTSTAPTDTTKPNDTTKPGNTNGSEKSPQTG DNSNIFLWFALLFVSAAGVTGITAYNKKKKEHAE
experiment.sample.molecule[0].mw127.857
experiment.sample.molecule[0].total_mw127.857
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe protein construct used for SAXS contains an additional C-terminal polyhistidine tag (LEHHHHHH).
experiment.sample.buffer.name50 mM Tris, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameIgA peptidase (A31-I1166; active site mutant E540A)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameMolecular Biology Institute of Barcelona
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameJuan Sebastián
experiment.contributor[0].contributor_surnameRamírez Larrota
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-05-23
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time4.0
experiment.number_of_frames250
experiment.wavelength0.099
experiment.sample_detector_distance2.867
experiment.concentration_minnull
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.063
experiment.s_max5.21
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDX42_fit1_model1_img.png
fits[0].models[0].softwareEOM/RANCH
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model1.cif
fits[0].models[0].model_mwnull
fits[0].models[0].bead_radius1.9
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fits[0].models[0].symmetrynull
fits[0].models[0].comment0.44 volume fraction
fits[0].models[0].user1196
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fits[0].models[1].model_mwnull
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fits[0].models[1].lognull
fits[0].models[1].symmetrynull
fits[0].models[1].comment0.11 volume fraction
fits[0].models[1].user1196
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fits[0].models[2].model_mwnull
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fits[0].models[2].symmetrynull
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fits[0].models[3].pdb_link[]
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fits[0].models[3].model_mwnull
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fits[0].models[3].symmetrynull
fits[0].models[3].comment0.22 volume fraction
fits[0].models[3].user1196
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fits[0].models[4].softwareEOM/RANCH
fits[0].models[4].pdb_link[]
fits[0].models[4].model_titlenull
fits[0].models[4].type_of_modelatomic
fits[0].models[4].software_versionnull
fits[0].models[4].model_datahttps://www.sasbdb.org/media/pdb_file/SASDX42_fit1_model5.cif
fits[0].models[4].model_mwnull
fits[0].models[4].bead_radius1.9
fits[0].models[4].lognull
fits[0].models[4].symmetrynull
fits[0].models[4].comment0.11 volume fraction
fits[0].models[4].user1196
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDX42_fit1_fixed_fit_img.png
fits[0].softwareEOM/GAJOE
fits[0].chi_square_value1.541
fits[0].p_value0.0139
fits[0].fit_residual_plotSASDX42_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDX42_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_9MVb3vS.log
fits[0].software_version2.1
fits[0].description
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pddf_softwareATSAS GNOM
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manifest.json:34 个字段值
字段路径原始值
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statussuccess
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
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    "title": "Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.",
    "publication": {
      "title": "Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2.",
      "author_list": "Ramírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1013292",
      "pmid": "40627637",
      "published_date": "2025 Jul"
    },
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    "submitted_date": "2025-02-27",
    "released_date": "2025-06-18"
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查看完整 manifest.json 原文
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