查看完整 summary.json 原文
{
"code": "SASDX66",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/A",
"project": {
"title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
"publication": {
"title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
"author_list": "Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS",
"journal": "Nat Commun",
"doi": "10.1038/s41467-025-62922-4",
"pmid": "40858555",
"published_date": "2025 Aug 26"
},
"status": "released",
"submitted_date": "2025-07-03",
"released_date": "2025-08-19"
},
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"name": "Pilatus3 S 1M, Pilatus3 900 K",
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},
"name": "National Synchrotron Light Source II (NSLS-II)",
"city": "Upton, NY",
"country": "USA",
"beamline_name": "16-ID (LiX)",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
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"sample": {
"molecule": [
{
"long_name": "Nucleoprotein",
"short_name": null,
"sequence": "MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
"organism": "Severe acute respiratory syndrome coronavirus 2",
"uniprot_code": "P0DTC9",
"uniprot_range_first": 2,
"uniprot_range_last": 419,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
"mw": 47.3,
"total_mw": 94.6,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "The protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG).\r\nN-terminal His6xtag+tev cleavage site"
}
],
"buffer": {
"name": "20 mM Tris, 150 mM NaCl",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
"deuteration": null
},
"purity_method": null,
"name": "Phosphorylated SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": null,
"full_name": "Rutgers University",
"webpage": null
}
],
"contributor_name": "Bruna",
"contributor_surname": "Favetta",
"orcid": "https://orcid.org/0000-0002-3613-1665"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-07-18",
"storage_temperature": 4.0,
"cell_temperature": 25.0,
"exposure_time": 0.5,
"number_of_frames": 10,
"wavelength": 0.08189,
"sample_detector_distance": 3.56,
"concentration_min": null,
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"s_min": 0.05,
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},
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"pddf_software": "BioXTAS RAW",
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"i0_calibration_standard": null,
"description": "Phosphorylated SARS-Cov-2 Nucleocapsid protein dimers at 1.5 mg/mL concentration in 150mM NaCl, 20mM Tris.\r\nPhosphorylation carried out in vitro using GSK3b and SRPK1 kinases.\r\nSAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1.\r\nλ = 0.08189 nm. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors.\r\nBackground subtraction was performed for each profile from its buffer.\r\nData collected at NSLS2 at Upton, NY.",
"experiment_description": null,
"tags": [],
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"guinier_point_first": 2,
"guinier_point_last": 19,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDX66_datloglog_img.png",
"symmetry": null,
"last_modified": "2025-09-01T15:11:15.799469+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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