查看完整 summary.json 原文
{
"code": "SASDX82",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "Activation and dimerization of the relaxase from the Bacillus subtilis conjugative plasmid pLS20 by DNA-induced cross-over of the catalytic residue",
"publication": null,
"status": "released",
"submitted_date": "2025-03-11",
"released_date": "2025-09-19"
},
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"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX82_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX82_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX82_pofr_img.png",
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"experiment": {
"instrument": {
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"name": "ADSC Quantum 210r",
"resolution": null
},
"name": "ALBA",
"city": "Cerdanyola del Vallès, Barcelona",
"country": "Spain",
"beamline_name": "BL11 - NCD",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
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"sample_path_length": null,
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},
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{
"long_name": "Relaxase",
"short_name": null,
"sequence": "MDSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSLSPVEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSQTGWVNEGAIQNSIRKGMEVLLREEQLEQSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRDKELSKISQLIHDRIAPKGLKFQVDKLAAALEHHHHHH",
"organism": "Bacillus subtilis subsp. natto",
"uniprot_code": "E9RJ23",
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"uniprot_range_last": 232,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MDSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSS\r\nGIFTQHKDSLSPVEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSQTGWVNEG\r\nAIQNSIRKGMEVLLREEQLEQSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTG\r\nEVYQARRGNRKLKTLDKMKSKVANTLMDRDKELSKISQLIHDRIAPKGLKFQPRLDTYMT\r\nKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQKYHPEDYKELDQSLKEEMEF\r\nRKSVYGDGPKQVERYKEYRKNKHKELYTKLGNSMLKEMAEIRRRESQSKQMNRSGTYAPS\r\nSVSNRQWDTKGRRIKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR",
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"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "The protein construct used for SAXS contains an additional non-native 15 amino acid C-terminal extension including a polyhistidine tag (VDKLAAALEHHHHHH)."
}
],
"buffer": {
"name": "20 mM Tris, 300 mM NaCl, 1 mM DTT",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "N-terminal domain of relaxase from Bacillus subtilis conjugative plasmid pLS20",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Barcelona, Spain",
"full_name": "ALBA - Cells",
"webpage": "https://www.cells.es"
}
],
"contributor_name": "Isidro",
"contributor_surname": "Crespo",
"orcid": "https://orcid.org/0000-0001-7698-1720"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2019-12-03",
"storage_temperature": 4.0,
"cell_temperature": 25.0,
"exposure_time": 0.1,
"number_of_frames": 20,
"wavelength": 0.1,
"sample_detector_distance": 2.56,
"concentration_min": null,
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"s_min": 0.21,
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"seccolumn": null
},
"fits": [
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"models": [
{
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"software": "Phenix",
"pdb_link": [],
"model_title": null,
"type_of_model": "atomic",
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"bead_radius": null,
"log": null,
"symmetry": "Monomer",
"comment": "Monomer",
"user": 1088
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDX82_fit1_fixed_fit_img.png",
"software": "OLIGOMER",
"chi_square_value": 0.89,
"p_value": 0.0,
"fit_residual_plot": "SASDX82_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDX82_fit1.fit",
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"software_version": null,
"description": ""
}
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"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
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"description": "pLS20: https://doi.org/10.1093/nargab/lqab096",
"experiment_description": "Synchrotron SAXS data from solutions of the N-terminal domain of relaxase in 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH 8 were collected on the BL11-NCD beam line at ALBA (Cerdanyola del Vallès, Barcelona, Spain) using a ADSC Quantum 210r detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 25°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 26.45,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 39.9,
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"pddf_i0": 1.875,
"pddf_i0_error": null,
"guinier_i0": 1.91399,
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"pddf_rg": 2.279,
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"porod_volume": 48.4,
"porod_volume_error": null,
"estimated_volume": null,
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"guinier_point_first": 23,
"guinier_point_last": 124,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDX82_datloglog_img.png",
"symmetry": null,
"last_modified": "2026-04-14T12:48:12.254122+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
{
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"started_at": "2026-08-11T13:33:06.222166+00:00",
"finished_at": "2026-08-11T13:33:14.738435+00:00",
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