查看完整 summary.json 原文
{
"code": "SASDXC2",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/A",
"project": {
"title": "The role of kinase domain dimerization in EGFR activation",
"publication": null,
"status": "released",
"submitted_date": "2025-03-16",
"released_date": "2025-11-02"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXC2.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXC2.dat",
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"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXC2_kratky_img.png",
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"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXC2_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXC2.sascif",
"experiment": {
"instrument": {
"detector": {
"type": "Rigaku HyPix - 3000",
"name": "Rigaku PSAXS Nano",
"resolution": 100.0
},
"name": "Yale University",
"city": "New Haven",
"country": "United States",
"beamline_name": "Rigaku MicroMax-007HF",
"beam_geometry": null,
"type_of_source": "X-ray in house",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
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"line_collimation_integrationwidth": null,
"xray_energy": null,
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"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Receptor protein-tyrosine kinase (duplication mutant)",
"short_name": "KDD",
"sequence": "HHHHHHSSGVDLGTENLYFQSMGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLLVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQG",
"organism": "Homo sapiens",
"uniprot_code": "Q504U8",
"uniprot_range_first": 651,
"uniprot_range_last": 993,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEV\nVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALA\nVLSNYDANKTGLKELPMRNLQGQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRG\nKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKY\nSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEF\nKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFL\nLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISG\nNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVS\nCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHY\nIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS\nIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLR\nILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYV\nMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGM\nNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALES\nILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYM\nIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEE\nDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSF\nLQRYSSDPTGALTEDSIDDTFLPVPGEWLVWKQSCSSTSSTHSAAASLQCPSQVLPPASP\nEGETVADLQTQ",
"mw": 79.594,
"total_mw": 79.594,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "EGFR kinase domain duplication mutant. The purified construct consists of amino acids 643-976 appended to amino acids 651-993 using mature protein numbering (UniProt Q504U8 numbering: 651-993 followed by a duplicated 643-977 region). The protein construct used for SAXS contains an additional N-terminal non-native 22 amino acid extension and polyhistidine tag (HHHHHHSSGVDLGTENLYFQSM)."
}
],
"buffer": {
"name": "20 mM HEPES, 250 mM NaCl, 250 mM KCl",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "EGFR kinase domain duplication mutant (KDD) at 1.6 mg/ml",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "New Haven, CT, USA",
"full_name": "Yale University",
"webpage": "https://www.yale.edu/"
}
],
"contributor_name": "Zaritza",
"contributor_surname": "Petrova",
"orcid": "https://orcid.org/0000-0002-4877-4676"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2022-03-22",
"storage_temperature": 4.0,
"cell_temperature": 4.0,
"exposure_time": 7200.0,
"number_of_frames": 3,
"wavelength": 0.1542,
"sample_detector_distance": 0.491,
"concentration_min": null,
"concentration_max": 1.6,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.078,
"s_max": 5.067,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDXC2_fit1_model1_img.png",
"software": "DAMMIF",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": "dammif (r10553)",
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDXC2_fit1_model1.pdb",
"model_mw": 85.6,
"bead_radius": 1.1,
"log": null,
"symmetry": null,
"comment": null,
"user": 1176
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXC2_fit1_fixed_fit_img.png",
"software": "DAMMIN",
"chi_square_value": 1.075,
"p_value": 0.3629,
"fit_residual_plot": "SASDXC2_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDXC2_fit1.fir",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/S_A_KDD_1_1_inPaper_truncateds_16.log",
"software_version": null,
"description": ""
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "SAXS data from solutions of EGFR kinase domain duplication mutant in 20 mM HEPES, 250 mM NaCl, 250 mM KCl, pH 8 were collected on a Rigaku MicroMax-007HF instrument (Yale University, New Haven, United States) using a Rigaku PSAXS Nano detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.1542 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.60 mg/ml was measured at 4°C. Three successive 7200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 79.6,
"experimental_mw_error": null,
"guinier_i0_mw": 79.4,
"guinier_i0_mw_error": null,
"porod_mw": 85.0,
"porod_mw_error": null,
"pddf_i0": 0.1557,
"pddf_i0_error": null,
"guinier_i0": 0.157258,
"guinier_i0_error": null,
"pddf_rg": 3.96,
"pddf_rg_error": null,
"guinier_rg": 3.941,
"guinier_rg_error": 0.056,
"pddf_dmax": 13.0,
"pddf_dmax_error": null,
"porod_volume": 135.0,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 6,
"guinier_point_last": 29,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDXC2_datloglog_img.png",
"symmetry": null,
"last_modified": "2025-11-02T21:11:41.959170+01:00",
"bragg_peak": []
}查看完整 manifest.json 原文
{
"code": "SASDXC2",
"status": "success",
"started_at": "2026-08-11T13:35:52.722219+00:00",
"finished_at": "2026-08-11T13:36:03.888133+00:00",
"source_last_modified": "2025-11-02T21:11:41.959170+01:00",
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