SASDXC7

Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-11-14T09:57:32.364238+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex

浓度— – 1.16 缓冲液 / pH20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol / 7.5
Experimental temperature20.0 设备 / 束线ESRF / BM29
波长0.9794 nm曝光1.0 s × 10

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Anti-Silencing Function 1
查看序列
MASLTKINSLFPNPSTMNDGYSFEIYINVSEQLQEDIGIKVIYVGSSFSNEYDQILEDIAVGPLSVGLNKFTITTGPIDMSKLPDDEILGNTLILISASYKDKEFCRIGYYVNNEFEGIDPTIDVVKREMLVPEKITRTLSEPRVTLFPCYWDKEEHIEMIEEKPEELGRVISFSADGTIQSNETENDNQMEEIQLNPEIKNQIRSALGVDGLMKKQKNMDEDDSEDEMEPMEQLEHHHHHH
proteinEntamoeba histolytica strain HM-1:IMSSC4LX081–234monomer分子数 127.69 kDa
Histone H3
查看序列
MARTKGHIERPSNKSAKAVKNVAFKAAKKMLSKDSTKKKRAHPGAVALTEIKVLQRSTELLLRKAPFQALVREIAQVSKSDLRFQSAAISALQEAAEAYLVGLFEDTNLCAIHAKRITIMPKDMQLARRIRGERT
proteinEntamoeba histolytica strain HM-1:IMSSQ061961–135monomer分子数 115.0 kDa
Histone H4
查看序列
MATDTGSGRGKGGKGVTLGKGSKGAKASKGGKRIRTKTQQDALKGITKPAIRRLARRGGVKRINGAVYDETRNVLKQFLEQVIRDSVTYTEHAKRRTVTAMDVVYALKRQGRTLYGYS
proteinEntamoeba histolytica strain HM-1:IMSSP402871–118monomer分子数 112.89 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11000[3]0.06285052554875252–5.209716520631418 1/nm含误差列缺失 041sasbdb/entries/c7/sasdxc7/source/SASDXC7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.00.2nm
i0Guinier37.20461/cm
i0P(r)37.20.151/cm
mwExperimental55.62kDa
mwPorod52.21kDa
porod_volumePorod83.55nm³
rgGuinier2.930.029nm
rgP(r)2.930.1nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-10-01储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数10
波长0.9794样品-探测器距离2.867
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q range0.063 – 5.21样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/c7/sasdxc7/source/SASDXC7.outdownloaded5026322e198cbbf68818db25cce4bf5f90d6737ec9b34249a93b44a9fe470919b6055Download查看原文件源站
sascifsasbdb/entries/c7/sasdxc7/source/SASDXC7.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/c7/sasdxc7/source/summary.jsondownloaded10792934ad26d0786ac5bb1d4a828249dc6b7eb8f61116af2154288044b5197316e23Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(8 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:236 个字段值
字段路径原始值
codeSASDXC7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleCharacterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.
project.publication.titleCharacterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.
project.publication.author_listGandhi S, Vasudevan D
project.publication.journalBiochimie
project.publication.doi10.1016/j.biochi.2025.11.003
project.publication.pmid41232797
project.publication.published_date2025 Nov 11
project.statusreleased
project.submitted_date2025-08-04
project.released_date2025-11-14
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXC7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXC7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXC7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXC7_kratky_img.png
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXC7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXC7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAnti-Silencing Function 1
experiment.sample.molecule[0].short_nameASF1
experiment.sample.molecule[0].sequenceMASLTKINSLFPNPSTMNDGYSFEIYINVSEQLQEDIGIKVIYVGSSFSNEYDQILEDIAVGPLSVGLNKFTITTGPIDMSKLPDDEILGNTLILISASYKDKEFCRIGYYVNNEFEGIDPTIDVVKREMLVPEKITRTLSEPRVTLFPCYWDKEEHIEMIEEKPEELGRVISFSADGTIQSNETENDNQMEEIQLNPEIKNQIRSALGVDGLMKKQKNMDEDDSEDEMEPMEQLEHHHHHH
experiment.sample.molecule[0].organismEntamoeba histolytica strain HM-1:IMSS
experiment.sample.molecule[0].uniprot_codeC4LX08
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last234
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMASLTKINSLFPNPSTMNDGYSFEIYINVSEQLQEDIGIKVIYVGSSFSNEYDQILEDIA VGPLSVGLNKFTITTGPIDMSKLPDDEILGNTLILISASYKDKEFCRIGYYVNNEFEGID PTIDVVKREMLVPEKITRTLSEPRVTLFPCYWDKEEHIEMIEEKPEELGRVISFSADGTI QSNETENDNQMEEIQLNPEIKNQIRSALGVDGLMKKQKNMDEDDSEDEMEPMEQ
experiment.sample.molecule[0].mw27.69
experiment.sample.molecule[0].total_mw27.69
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionAnti-Silencing Function 1 (ASF1) protein from Entamoeba histolytica with hexa-histidine tag
experiment.sample.molecule[1].long_nameHistone H3
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceMARTKGHIERPSNKSAKAVKNVAFKAAKKMLSKDSTKKKRAHPGAVALTEIKVLQRSTELLLRKAPFQALVREIAQVSKSDLRFQSAAISALQEAAEAYLVGLFEDTNLCAIHAKRITIMPKDMQLARRIRGERT
experiment.sample.molecule[1].organismEntamoeba histolytica strain HM-1:IMSS
experiment.sample.molecule[1].uniprot_codeQ06196
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last135
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMARTKGHIERPSNKSAKAVKNVAFKAAKKMLSKDSTKKKRAHPGAVALTEIKVLQRSTEL LLRKAPFQALVREIAQVSKSDLRFQSAAISALQEAAEAYLVGLFEDTNLCAIHAKRITIM PKDMQLARRIRGERT
experiment.sample.molecule[1].mw15.0
experiment.sample.molecule[1].total_mw15.0
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.molecule[2].long_nameHistone H4
experiment.sample.molecule[2].short_namenull
experiment.sample.molecule[2].sequenceMATDTGSGRGKGGKGVTLGKGSKGAKASKGGKRIRTKTQQDALKGITKPAIRRLARRGGVKRINGAVYDETRNVLKQFLEQVIRDSVTYTEHAKRRTVTAMDVVYALKRQGRTLYGYS
experiment.sample.molecule[2].organismEntamoeba histolytica strain HM-1:IMSS
experiment.sample.molecule[2].uniprot_codeP40287
experiment.sample.molecule[2].uniprot_range_first1
experiment.sample.molecule[2].uniprot_range_last118
experiment.sample.molecule[2].oligomerizationmonomer
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequenceMATDTGSGRGKGGKGVTLGKGSKGAKASKGGKRIRTKTQQDALKGITKPAIRRLARRGGV KRINGAVYDETRNVLKQFLEQVIRDSVTYTEHAKRRTVTAMDVVYALKRQGRTLYGYS
experiment.sample.molecule[2].mw12.89
experiment.sample.molecule[2].total_mw12.89
experiment.sample.molecule[2].number_molecules1
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_description
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEntamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressIndia
experiment.contributor[0].affiliation[0].full_nameInstitute of Life Sciences (ILS)
experiment.contributor[0].affiliation[0].webpagehttps://www.ils.res.in/
experiment.contributor[0].contributor_nameSurajit
experiment.contributor[0].contributor_surnameGandhi
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-8258-2870
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-10-01
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames10
experiment.wavelength0.9794
experiment.sample_detector_distance2.867
experiment.concentration_minnull
experiment.concentration_max1.16
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.063
experiment.s_max5.21
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].model_mw51.4
fits[0].models[0].bead_radius1.5
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fits[0].chi_square_value0.856
fits[0].p_value0.0594
fits[0].fit_residual_plotSASDXC7_fit1_fitresiduals_img.png
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fits[1].softwareFoXS
fits[1].chi_square_value0.975979
fits[1].p_value0.0072
fits[1].fit_residual_plotSASDXC7_fit2_fitresiduals_img.png
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fits[2].softwareDAMMIF
fits[2].chi_square_value0.838
fits[2].p_value0.0594
fits[2].fit_residual_plotSASDXC7_fit3_fitresiduals_img.png
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fits[2].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw55.62
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw52.21
porod_mw_errornull
pddf_i037.2
pddf_i0_error0.15
guinier_i037.2046
guinier_i0_errornull
pddf_rg2.93
pddf_rg_error0.1
guinier_rg2.93
guinier_rg_error0.029
pddf_dmax8.0
pddf_dmax_error0.2
porod_volume83.55
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first7
guinier_point_last73
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXC7_datloglog_img.png
symmetrynull
last_modified2025-11-14T09:57:32.364238+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXC7
statussuccess
started_at2026-08-11T16:32:23.909337+00:00
finished_at2026-08-11T16:32:32.394491+00:00
source_last_modified2025-11-14T09:57:32.364238+01:00
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查看完整 summary.json 原文
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    "title": "Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.",
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      "title": "Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.",
      "author_list": "Gandhi S, Vasudevan D",
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查看完整 manifest.json 原文
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