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{
"code": "SASDXC7",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.",
"publication": {
"title": "Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.",
"author_list": "Gandhi S, Vasudevan D",
"journal": "Biochimie",
"doi": "10.1016/j.biochi.2025.11.003",
"pmid": "41232797",
"published_date": "2025 Nov 11"
},
"status": "released",
"submitted_date": "2025-08-04",
"released_date": "2025-11-14"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXC7.out",
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"experiment": {
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"name": "Pilatus3 2M",
"resolution": 0.172
},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "point",
"type_of_source": "X-ray synchrotron",
"point_source": null,
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},
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{
"long_name": "Anti-Silencing Function 1",
"short_name": "ASF1",
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"organism": "Entamoeba histolytica strain HM-1:IMSS",
"uniprot_code": "C4LX08",
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"uniprot_range_last": 234,
"oligomerization": "monomer",
"molecular_type": "protein",
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"mw": 27.69,
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"molecule_source": "biological",
"molecule_description": "Anti-Silencing Function 1 (ASF1) protein from Entamoeba histolytica with hexa-histidine tag"
},
{
"long_name": "Histone H3",
"short_name": null,
"sequence": "MARTKGHIERPSNKSAKAVKNVAFKAAKKMLSKDSTKKKRAHPGAVALTEIKVLQRSTELLLRKAPFQALVREIAQVSKSDLRFQSAAISALQEAAEAYLVGLFEDTNLCAIHAKRITIMPKDMQLARRIRGERT",
"organism": "Entamoeba histolytica strain HM-1:IMSS",
"uniprot_code": "Q06196",
"uniprot_range_first": 1,
"uniprot_range_last": 135,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MARTKGHIERPSNKSAKAVKNVAFKAAKKMLSKDSTKKKRAHPGAVALTEIKVLQRSTEL\nLLRKAPFQALVREIAQVSKSDLRFQSAAISALQEAAEAYLVGLFEDTNLCAIHAKRITIM\nPKDMQLARRIRGERT",
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": ""
},
{
"long_name": "Histone H4",
"short_name": null,
"sequence": "MATDTGSGRGKGGKGVTLGKGSKGAKASKGGKRIRTKTQQDALKGITKPAIRRLARRGGVKRINGAVYDETRNVLKQFLEQVIRDSVTYTEHAKRRTVTAMDVVYALKRQGRTLYGYS",
"organism": "Entamoeba histolytica strain HM-1:IMSS",
"uniprot_code": "P40287",
"uniprot_range_first": 1,
"uniprot_range_last": 118,
"oligomerization": "monomer",
"molecular_type": "protein",
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": ""
}
],
"buffer": {
"name": "20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
"deuteration": null
},
"purity_method": null,
"name": "Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "India",
"full_name": "Institute of Life Sciences (ILS)",
"webpage": "https://www.ils.res.in/"
}
],
"contributor_name": "Surajit",
"contributor_surname": "Gandhi",
"orcid": "https://orcid.org/0000-0002-8258-2870"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2023-10-01",
"storage_temperature": 4.0,
"cell_temperature": 20.0,
"exposure_time": 1.0,
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"wavelength": 0.9794,
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},
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"last_modified": "2025-11-14T09:57:32.364238+01:00",
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}