SASDXG8

Streptococcus agalactiae IgA FC receptor

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-06-16T09:24:10.659492+02:00

Synchrotron small-angle X-ray scattering (SAXS) data on Streptococcus agalactiae β-Antigen C Protein were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Streptococcus agalactiae IgA FC receptor

Concentration— – — Buffer / pH20mM HEPES, 140mM NaCl / 7.4
Experimental temperature— Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
IgA FC receptor β-Antigen C Protein
View sequence
MGSSHHHHHHSSGLVPRGSQHMGMEQKLISEEDLNGENLYFQGSTGSKAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEELDKATTNEQATQVKNQFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKKRVKRDLAANENNQQKIELTVSPENITVYEGEDVKFTVTAKSDSKTTLDFSDLLTKYNPSVSDRISTNYKTNTDNHKIAEITIKNLKLNESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQVPKTPEQKDSKTEEKVPQEPKSNDKNQLQELIKSAQQELEKLEKAIKELMEQPEIPSNPEYGIQKSIWESQKEPIQEAITSFKKIIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTRKVVQYMNKYPDNAEIKKIFESDMKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDDLDKKVEQDQPAPIPENSEMDQAKEKAKIAVSKYMSKVLDGVHQHLQKKNHSKIVDLFKELEAIKQQTIFDIDNAKTEVEIDNLVHDAFSKMNATVAKFQKGLETNT
proteinStreptococcus agalactiaeP27951258–826monomerMolecule count 170.944 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1913[3]0.010896–0.472902 1/AHas uncertainty columnMissing 023sasbdb/entries/g8/sasdxg8/source/SASDXG8.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)20.2—Å
i0Guinier74.8732—arbitrary
i0P(r)71.62—arbitrary
mwExperimental65.3—kDa
mwPorod87.0—kDa
porod_volumePorod140.0—ų
rgGuinier5.894.734Å
rgP(r)5.891.2Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM HEPES, 140mM NaClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-10-05Storage / measurement temperature— / —
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.109 – 4.729Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:150 field values
Field pathRaw value
codeSASDXG8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.author_listRumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-026-74098-6
project.publication.pmid42277001
project.publication.published_date2026 Jun 11
project.statusreleased
project.submitted_date2024-07-11
project.released_date2026-06-16
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIgA FC receptor β-Antigen C Protein
experiment.sample.molecule[0].short_nameβ-Antigen C Protein
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSQHMGMEQKLISEEDLNGENLYFQGST GSKAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEELDKATTNEQATQVKN QFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREY EGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKKRVKRDLAANENNQQK IELTVSPENITVYEGEDVKFTVTAKSDSKTTLDFSDLLTKYNPSVSDRISTNYKTNTDNH KIAEITIKNLKLNESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQVPKTPEQKDSKTEEK VPQEPKSNDKNQLQELIKSAQQELEKLEKAIKELMEQPEIPSNPEYGIQKSIWESQKEPI QEAITSFKKIIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTRKVVQYMNKYPDNAEI KKIFESDMKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDDLDKKVEQDQPAPIPE NSEMDQAKEKAKIAVSKYMSKVLDGVHQHLQKKNHSKIVDLFKELEAIKQQTIFDIDNAK TEVEIDNLVHDAFSKMNATVAKFQKGLETNT
experiment.sample.molecule[0].organismStreptococcus agalactiae
experiment.sample.molecule[0].uniprot_codeP27951
experiment.sample.molecule[0].uniprot_range_first258
experiment.sample.molecule[0].uniprot_range_last826
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFKSNYERKMRYSIRKFSVGVASVAVASLFMGSVAHASELVKDDSVKTTEVAAKPYPSMA QTDQGNNSSSSELETTKMEIPTTDIKKAVEPVEKTAGETSATDTGKREKQLQQWKNNLKN DVDNTILSHEQKNEFKTKIDETNDSDALLELENQFNETNRLLHIKQHEEVEKDKKAKQQK TLKQSDTKVDLSNIDKELNHQKSQVEKMAEQKGITNEDKDSMLKKIEDIRKQAQQADKKE DAEVKVREELGKLFSSTKAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEE LDKATTNEQATQVKNQFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEAN LEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKK RVKRDLAANENNQQKIELTVSPENITVYEGEDVKFTVTAKSDSKTTLDFSDLLTKYNPSV SDRISTNYKTNTDNHKIAEITIKNLKLNESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQ VPKTPEQKDSKTEEKVPQEPKSNDKNQLQELIKSAQQELEKLEKAIKELMEQPEIPSNPE YGIQKSIWESQKEPIQEAITSFKKIIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTR KVVQYMNKYPDNAEIKKIFESDMKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDD LDKKVEQDQPAPIPENSEMDQAKEKAKIAVSKYMSKVLDGVHQHLQKKNNSKIVDLFKEL EAIKQQTIFDIDNAKTEVEIDNLVHDAFSKMNATVAKFQKGLETNTPETPDTPKIPELPQ APDTPQAPDTPHVPESPKAPEAPRVPESPKTPEAPHVPESPKAPEAPRVPESPKTPEAPH VPESPKTPEAPKIPEPPKTPDVPKLPDVPKLPDVPKLPDAPKLPDGLNKVGQAVFTSTDG NTKVTVVFDKPTDADKLHLKEVTTKELADKIAHKTGGGTVRVFDLSLSKGGKETHVNGER TVRLALGQTGSDVHVYHVKENGDLERIPSKVENGQVVFKTNHFSLFAIKTLSKDQNVTPP KQTKPSTQGSQVEIAESQTGKFQSKAANHKALATGNETVAKGNPTSTTEKKLPYTGVASN LVLEIMGLLGLIGTSFIAMKRRKS
experiment.sample.molecule[0].mw70.944
experiment.sample.molecule[0].total_mw70.944
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20mM HEPES, 140mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
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experiment.sample.purity_methodnull
experiment.sample.nameStreptococcus agalactiae IgA FC receptor
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
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experiment.date2023-10-05
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fits[0].p_value0.0034
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSynchrotron small-angle X-ray scattering (SAXS) data on Streptococcus agalactiae β-Antigen C Protein were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.
experiment_description-
tags[]
intensity_unitarbitrary
experimental_mw65.3
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw87.0
porod_mw_errornull
pddf_i071.62
pddf_i0_errornull
guinier_i074.8732
guinier_i0_errornull
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pddf_rg_error1.2
guinier_rg5.89
guinier_rg_error4.734
pddf_dmax20.2
pddf_dmax_errornull
porod_volume140.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last23
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXG8_datloglog_img.png
symmetrynull
last_modified2026-06-16T09:24:10.659492+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXG8
statussuccess
started_at2026-08-11T16:41:37.655340+00:00
finished_at2026-08-11T16:41:45.892226+00:00
source_last_modified2026-06-16T09:24:10.659492+02:00
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View the complete summary.json source
{
  "code": "SASDXG8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
    "publication": {
      "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
      "author_list": "Rumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-74098-6",
      "pmid": "42277001",
      "published_date": "2026 Jun 11"
    },
    "status": "released",
    "submitted_date": "2024-07-11",
    "released_date": "2026-06-16"
  },
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXG8_pofr_img.png",
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    "sample": {
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        {
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          "organism": "Streptococcus agalactiae",
          "uniprot_code": "P27951",
          "uniprot_range_first": 258,
          "uniprot_range_last": 826,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "20mM HEPES, 140mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Streptococcus agalactiae IgA FC receptor",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-10-05",
    "storage_temperature": null,
    "cell_temperature": null,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": null,
    "concentration_max": null,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.109,
    "s_max": 4.729,
    "total_exposure_time": null,
    "seccolumn": null
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    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDXG8_fit1_model1_img.png",
          "software": "FoXSDock",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDXG8_fit1_model1.pdb",
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          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 1086
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXG8_fit1_fixed_fit_img.png",
      "software": "FoXS",
      "chi_square_value": 0.838836,
      "p_value": 0.0034,
      "fit_residual_plot": "SASDXG8_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDXG8_fit1.fit",
      "fit_log": null,
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      "description": ""
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  "description": "Synchrotron small-angle X-ray scattering (SAXS) data on Streptococcus agalactiae β-Antigen C Protein were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.",
  "experiment_description": "-",
  "tags": [],
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  "guinier_rg_error": 4.734,
  "pddf_dmax": 20.2,
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  "porod_volume": 140.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 23,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXG8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-06-16T09:24:10.659492+02:00",
  "bragg_peak": []
}
View the complete manifest.json source
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