SASDXH8

Leukocyte Immunoglobulin-Like Receptor LILR-B3

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-06-16T09:24:11.486470+02:00

Synchrotron small-angle X-ray scattering (SAXS) data on Leukocyte Immunoglobulin-Like Receptor (LILR-B3) were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Leukocyte Immunoglobulin-Like Receptor LILR-B3

Concentration— – — Buffer / pH20mM HEPES, 140mM NaCl / 7.4
Experimental temperature— Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)
View sequence
GPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSRSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH
proteinHomo sapiensA0A0G2JM4624–443monomerMolecule count 146.951 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1913[3]0.010896–0.472902 1/AHas uncertainty columnMissing 019sasbdb/entries/h8/sasdxh8/source/SASDXH8.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.5—Å
i0Guinier27.5635—arbitrary
i0P(r)28.370.27arbitrary
mwExperimental57.8—kDa
mwPorod85.0—kDa
porod_volumePorod137.0—ų
rgGuinier3.60.481Å
rgP(r)3.810.42Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM HEPES, 140mM NaClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-10-05Storage / measurement temperature— / —
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.109 – 4.729Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDXH8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.author_listRumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-026-74098-6
project.publication.pmid42277001
project.publication.published_date2026 Jun 11
project.statusreleased
project.submitted_date2024-07-11
project.released_date2026-06-16
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameLeukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)
experiment.sample.molecule[0].short_nameLILRB3
experiment.sample.molecule[0].sequenceGPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSRSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeA0A0G2JM46
experiment.sample.molecule[0].uniprot_range_first24
experiment.sample.molecule[0].uniprot_range_last443
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTPALTALLCLGLSLGPRTRVQAGPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRL DKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGF YNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQAL FPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQS LTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSPSHGGQYRCYGAH NLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEG AAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSG HSGGSSLPPTGPPSTPGLGRYLEVLIGVSVAFVLLLFLLLFLLLRRQRHSKHRTSDQRKT DFQRPAGAAETEPKDRGLLRRSSPAADVQEENLYAAVKDTQSEDRVELDSQQSPHDEDPQ AVTYAPVKHSSPRREMASPPSSLSGEFLDTKDRQVEEDRQMDTEAAASEASQDVTYAQLH SLTLRRKATEPPPSQEGEPPAEPSIYATLAIH
experiment.sample.molecule[0].mw46.951
experiment.sample.molecule[0].total_mw46.951
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe protein construct used for SAXS contains an additional non-native polyhistidine tag at the C-terminus (HHHHHH).
experiment.sample.buffer.name20mM HEPES, 140mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLeukocyte Immunoglobulin-Like Receptor LILR-B3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameYing
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidhttps://orcid.org/0009-0003-1603-7561
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-10-05
experiment.storage_temperaturenull
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experiment.sample_volumenull
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fits[0].softwareFoXS
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fits[0].p_value0.0
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSynchrotron small-angle X-ray scattering (SAXS) data on Leukocyte Immunoglobulin-Like Receptor (LILR-B3) were collected at SIBYLS beamline 12.3.1 of the Advanced Light Source in SEC-SAXS mode. In-line size-exclusion chromatography (SEC) was performed using an Agilent 1260 HPLC system. The SEC conditions were as follows: a 60 µL sample at 5 mg/mL was injected onto a Shodex KW-803 column at 4 °C that had been previously equilibrated in 20 mM HEPES, 140 mM NaCl, pH 7.4, and eluted at 0.65 mL/min. Scattering was recorded on a Pilatus3 X 2M detector at a sample-to-detector distance of 2.1 m and a wavelength of λ = 0.1127 nm (I(q) vs q; q = 4π sin θ/λ, where 2θ is the scattering angle). 913 successive 2-s frames were collected. The scattering curves were normalized to the transmitted-beam intensity, radially averaged, and corrected by subtracting the solvent blank.
experiment_description-
tags[]
intensity_unitarbitrary
experimental_mw57.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw85.0
porod_mw_errornull
pddf_i028.37
pddf_i0_error0.27
guinier_i027.5635
guinier_i0_errornull
pddf_rg3.81
pddf_rg_error0.42
guinier_rg3.6
guinier_rg_error0.481
pddf_dmax13.5
pddf_dmax_errornull
porod_volume137.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last50
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXH8_datloglog_img.png
symmetrynull
last_modified2026-06-16T09:24:11.486470+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXH8
statussuccess
started_at2026-08-11T16:27:50.392499+00:00
finished_at2026-08-11T16:27:58.433370+00:00
source_last_modified2026-06-16T09:24:11.486470+02:00
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View the complete summary.json source
{
  "code": "SASDXH8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
    "publication": {
      "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
      "author_list": "Rumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-74098-6",
      "pmid": "42277001",
      "published_date": "2026 Jun 11"
    },
    "status": "released",
    "submitted_date": "2024-07-11",
    "released_date": "2026-06-16"
  },
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      "country": "USA",
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      "molecule": [
        {
          "long_name": "Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)",
          "short_name": "LILRB3",
          "sequence": "GPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSRSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH",
          "organism": "Homo sapiens",
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      "name": "Leukocyte Immunoglobulin-Like Receptor LILR-B3",
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    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Kansas State University",
            "webpage": null
          }
        ],
        "contributor_name": "Ying",
        "contributor_surname": "Zhang",
        "orcid": "https://orcid.org/0009-0003-1603-7561"
      }
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}
View the complete manifest.json source
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