Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 14 as the linker region + 10 nM Ca2+/CaM

{
  "code": "SASDXK5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation - revision",
    "publication": null,
    "status": "released",
    "submitted_date": "2025-07-01",
    "released_date": "2025-09-03"
  },
  "pddf_data": null,
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  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXK5_dat_img.png",
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  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXK5_guinier_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Reverse-biased silicon diode array",
        "name": "DECTRIS/ PILATUS 100K",
        "resolution": 172.0
      },
      "name": "Institute for Applied Life Sciences/Biophysical Characterization",
      "city": "Amherst",
      "country": "United States",
      "beamline_name": "Rigaku/BioSAXS-2000 SAXS",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
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      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344)",
          "short_name": "CaMKII Exon 14",
          "sequence": "TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
          "organism": "Homo sapiens",
          "uniprot_code": "Q9UQM7",
          "uniprot_range_first": 7,
          "uniprot_range_last": 478,
          "oligomerization": "dodecamer",
          "molecular_type": "protein",
          "uniprot_sequence": "MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH",
          "mw": 51.699,
          "total_mw": 620.388,
          "number_molecules": 12,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The linker region only contains Exon 14."
        }
      ],
      "buffer": {
        "name": "50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP",
        "concentration_unit": null,
        "comment": "no caM",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 14 as the linker region + 10 nM Ca2+/CaM",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "university of massachusetts amherst",
            "webpage": null
          }
        ],
        "contributor_name": "Bao",
        "contributor_surname": "Nguyen Viet",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-12-18",
    "storage_temperature": 10.0,
    "cell_temperature": 22.0,
    "exposure_time": 600.0,
    "number_of_frames": 1,
    "wavelength": 0.154,
    "sample_detector_distance": 0.4389,
    "concentration_min": null,
    "concentration_max": 1.5,
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    "flow_rate": null,
    "s_min": 0.066,
    "s_max": 6.962,
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    "seccolumn": null
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
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  "guinier_rg_error": 2.484,
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  "estimated_volume_error": null,
  "guinier_point_first": 3,
  "guinier_point_last": 8,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2025-09-03T03:50:25.100624+02:00",
  "bragg_peak": []
}

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  "finished_at": "2026-08-11T14:50:07.400834+00:00",
  "source_last_modified": "2025-09-03T03:50:25.100624+02:00",
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