A nuclear mRNA-binding protein bound to GT-repeat DNA Oligo: TDP-43 (TAR DNA-binding protein 43)

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  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "From TDP-43/RNA complex formation to disease-linked TDP-43 aggregation through a structural and cellular approach.",
    "publication": {
      "title": "From TDP-43/RNA complex formation to disease-linked TDP-43 aggregation through a structural and cellular approach.",
      "author_list": "Feng Y, Joshi V, Pankivskyi S, Clément MJ, Rengifo-Gonzalez JC, Thureau A, Pastré D, Bouhss A",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-68346-y",
      "pmid": "41565639",
      "published_date": "2026 Jan 21"
    },
    "status": "released",
    "submitted_date": "2025-09-08",
    "released_date": "2026-01-25"
  },
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        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
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      "name": "SOLEIL",
      "city": "Saint-Aubin",
      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
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      "molecule": [
        {
          "long_name": "TAR DNA-binding protein 43",
          "short_name": "TDP-43 - WT",
          "sequence": "MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFG",
          "organism": "Homo sapiens",
          "uniprot_code": "Q13148",
          "uniprot_range_first": 1,
          "uniprot_range_last": 277,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGI\nLHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDL\nKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNS\nKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIA\nQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLG\nNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQ\nREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM",
          "mw": 31.5,
          "total_mw": 63.0,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "RNA-binding protein that is involved in various steps of RNA biogenesis and processing.\r\nCo-exist in a mixure of monomere and dimer"
        }
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        "name": "20 mM HEPES, 100 mM KCl, 2 mM TCEP",
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        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.6,
        "deuteration": null
      },
      "purity_method": null,
      "name": "A nuclear mRNA-binding protein bound to GT-repeat DNA Oligo: TDP-43 (TAR DNA-binding protein 43)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "Soleil",
            "address": "Saint-Aubin, France",
            "full_name": "Soleil Synchrotron",
            "webpage": "http://www.synchrotron-soleil.fr/"
          }
        ],
        "contributor_name": "Aurélien",
        "contributor_surname": "Thureau",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2022-11-30",
    "storage_temperature": 10.0,
    "cell_temperature": 25.0,
    "exposure_time": 1.0,
    "number_of_frames": 600,
    "wavelength": 0.1033,
    "sample_detector_distance": 2.0,
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    "concentration_max": 1.0,
    "sample_volume": 50.0,
    "flow_rate": 0.3,
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    "s_max": 5.516,
    "total_exposure_time": null,
    "seccolumn": 34
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
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  "description": "15 µL of ssDNA (GT)12 was incubated with 50 µL of TDP-43 during 1 hour at room temperature to reach a final ratio for the complex TDP-43:ssDNA of 2:1 with 60 µM of TDP-43 and 27 µM of ssDNA. 50 µL of this complex was injected into the SEC column.",
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  "tags": [],
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  "guinier_rg_error": 0.261,
  "pddf_dmax": 16.5,
  "pddf_dmax_error": null,
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  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 23,
  "guinier_point_last": 50,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXL8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-01-25T12:12:53.995549+01:00",
  "bragg_peak": []
}

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