SASDXQ7

Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-03-16T15:52:20.837179+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP / 7.5
Experimental temperature20.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline
波长1.10367 nm曝光1.0 s × 500

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Human lung-associated vientovirus FB Replication-Associated Protein Hexamer
查看序列
MTGGSLRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAPMEEESMFPEEWSVIEPEGFITPG
proteinVitentovirus RB1–350hexamer分子数 640611.71 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11934[3]0.00806870594–0.487970508 1/A含误差列缺失 01296sasbdb/entries/q7/sasdxq7/source/SASDXQ7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)144.0Å
i0Guinier5.629151/cm
i0P(r)5.620.0261/cm
mwExperimental358.0kDa
mwPorod305.0kDa
porod_volumePorod488.0ų
rgGuinier4.6970.419Å
rgP(r)4.680.022Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-01-25储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数500
波长1.10367样品-探测器距离1.713
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline · Ithaca, NY, USA
q range0.081 – 4.88样品体积 / 流速100.0 / 0.5

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/q7/sasdxq7/source/SASDXQ7.outdownloaded62681638519d233c607ccb9977861f3c814b1f454ed0ff971565f9d71f5d8c6f873ceDownload查看原文件源站
sascifsasbdb/entries/q7/sasdxq7/source/SASDXQ7.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/q7/sasdxq7/source/summary.jsondownloaded5756a93be2397fc5b5f95966cef3478af9a64fe3fae52fa94c382054719ff2f8f791Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDXQ7
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.author_listMontermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1013997
project.publication.pmid41779823
project.publication.published_date2026 Mar
project.statusreleased
project.submitted_date2025-08-11
project.released_date2026-02-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXQ7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXQ7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXQ7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXQ7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameID7A1 BioSAXS / HP-Bio Beamline
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman lung-associated vientovirus FB Replication-Associated Protein Hexamer
experiment.sample.molecule[0].short_nameRep
experiment.sample.molecule[0].sequenceMTGGSLRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAP MEEESMFPEEWSVIEPEGFITPG
experiment.sample.molecule[0].organismVitentovirus RB
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last350
experiment.sample.molecule[0].oligomerizationhexamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw40611.71
experiment.sample.molecule[0].total_mw243670.26
experiment.sample.molecule[0].number_molecules6
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameKushol
experiment.contributor[0].contributor_surnameGupta
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-7006-2667
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-01-25
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames500
experiment.wavelength1.10367
experiment.sample_detector_distance1.713
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume100.0
experiment.flow_rate0.5
experiment.s_min0.081
experiment.s_max4.88
experiment.total_exposure_timenull
experiment.seccolumn2
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw358.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw305.0
porod_mw_errornull
pddf_i05.62
pddf_i0_error0.026
guinier_i05.62915
guinier_i0_errornull
pddf_rg4.68
pddf_rg_error0.022
guinier_rg4.697
guinier_rg_error0.419
pddf_dmax144.0
pddf_dmax_errornull
porod_volume488.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last78
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXQ7_datloglog_img.png
symmetrynull
last_modified2026-03-16T15:52:20.837179+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXQ7
statussuccess
started_at2026-08-11T15:36:57.147462+00:00
finished_at2026-08-11T15:37:06.204632+00:00
source_last_modified2026-03-16T15:52:20.837179+01:00
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查看完整 summary.json 原文
{
  "code": "SASDXQ7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
    "publication": {
      "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
      "author_list": "Montermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1013997",
      "pmid": "41779823",
      "published_date": "2026 Mar"
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    "status": "released",
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    "released_date": "2026-02-16"
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}
查看完整 manifest.json 原文
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