SASDXQ7

Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-03-16T15:52:20.837179+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses

Concentration— – 5.0 Buffer / pH20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP / 7.5
Experimental temperature20.0 Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline
Wavelength1.10367 nmExposure1.0 s × 500

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Human lung-associated vientovirus FB Replication-Associated Protein Hexamer
View sequence
MTGGSLRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAPMEEESMFPEEWSVIEPEGFITPG
proteinVitentovirus RB—1–350hexamerMolecule count 640611.71 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11934[3]0.00806870594–0.487970508 1/AHas uncertainty columnMissing 01296sasbdb/entries/q7/sasdxq7/source/SASDXQ7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)144.0—Å
i0Guinier5.62915—1/cm
i0P(r)5.620.0261/cm
mwExperimental358.0—kDa
mwPorod305.0—kDa
porod_volumePorod488.0—ų
rgGuinier4.6970.419Å
rgP(r)4.680.022Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 300 mM NaCl, 0.1 mM TCEPBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-01-25Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames500
Wavelength1.10367Sample-to-detector distance1.713
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline · Ithaca, NY, USA
q range0.081 – 4.88Sample volume / flow rate100.0 / 0.5

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDXQ7
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.author_listMontermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1013997
project.publication.pmid41779823
project.publication.published_date2026 Mar
project.statusreleased
project.submitted_date2025-08-11
project.released_date2026-02-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXQ7.out
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameID7A1 BioSAXS / HP-Bio Beamline
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman lung-associated vientovirus FB Replication-Associated Protein Hexamer
experiment.sample.molecule[0].short_nameRep
experiment.sample.molecule[0].sequenceMTGGSLRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAP MEEESMFPEEWSVIEPEGFITPG
experiment.sample.molecule[0].organismVitentovirus RB
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last350
experiment.sample.molecule[0].oligomerizationhexamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw40611.71
experiment.sample.molecule[0].total_mw243670.26
experiment.sample.molecule[0].number_molecules6
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameKushol
experiment.contributor[0].contributor_surnameGupta
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-7006-2667
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-01-25
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames500
experiment.wavelength1.10367
experiment.sample_detector_distance1.713
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume100.0
experiment.flow_rate0.5
experiment.s_min0.081
experiment.s_max4.88
experiment.total_exposure_timenull
experiment.seccolumn2
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estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw358.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw305.0
porod_mw_errornull
pddf_i05.62
pddf_i0_error0.026
guinier_i05.62915
guinier_i0_errornull
pddf_rg4.68
pddf_rg_error0.022
guinier_rg4.697
guinier_rg_error0.419
pddf_dmax144.0
pddf_dmax_errornull
porod_volume488.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last78
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXQ7_datloglog_img.png
symmetrynull
last_modified2026-03-16T15:52:20.837179+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXQ7
statussuccess
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finished_at2026-08-11T15:37:06.204632+00:00
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View the complete summary.json source
{
  "code": "SASDXQ7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
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    "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
    "publication": {
      "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
      "author_list": "Montermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1013997",
      "pmid": "41779823",
      "published_date": "2026 Mar"
    },
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    "released_date": "2026-02-16"
  },
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}
View the complete manifest.json source
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