SASDXS7

Human lung-associated vientovirus FB Replication-Associated Protein ADP-bound (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-03-16T15:52:53.563334+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Human lung-associated vientovirus FB Replication-Associated Protein ADP-bound (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP / 7.5
实验温度20.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline
波长1.10367 nm曝光1.0 s × 500

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Human lung-associated vientovirus FB Replication-Associated Protein Dodecamer
查看序列
MTGGRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAPMEEESMFPEEWSVIEPEGFITPG
proteinVientovirus FB1–350dodecamer分子数 1240611.71 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11934[3]0.00806870594–0.487970508 1/A含误差列缺失 0480sasbdb/entries/s7/sasdxs7/source/SASDXS7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)239.0Å
i0Guinier6.221151/cm
i0P(r)6.320.051/cm
mwExperimental371.0kDa
mwPorod530.0kDa
porod_volumePorod850.0ų
rgGuinier5.4840.425Å
rgP(r)5.7380.09Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-01-25储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数500
波长1.10367样品-探测器距离1.713
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / ID7A1 BioSAXS / HP-Bio Beamline · Ithaca, NY, USA
q 范围0.081 – 4.88样品体积 / 流速100.0 / 0.5

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/s7/sasdxs7/source/SASDXS7.datdownloaded964856f6cf0d38d17f6300c801e6ae16d5a19eab5d1fe5cb3a9edfbbdf10c077cb178下载查看原文件源站
full_entry_zipsasbdb/entries/s7/sasdxs7/source/SASDXS7.zipdownloaded48480a9ffc4f5ef1fa1b157f46c51f599e89b0304b9c71c6c24200330ccf7dce659bf下载查看原文件源站
pddfsasbdb/entries/s7/sasdxs7/source/SASDXS7.outdownloaded53368f29d170d0059368497e53a6f4503ba5afaab57c662b3a975a384f1f9522139fe下载查看原文件源站
sascifsasbdb/entries/s7/sasdxs7/source/SASDXS7.sascifnot_available下载查看原文件源站
summarysasbdb/entries/s7/sasdxs7/source/summary.jsondownloaded5778794b896d5a4f14f9f05002e4f91c6e8d0a31d9281c0b050fbf1338aca83f473b下载查看原文件源站
curve:来源记录
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  "url": "https://www.sasbdb.org/media/intensities_files/SASDXS7.dat"
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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  "status": "downloaded",
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  "zip_member_count": 2,
  "zip_members": [
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}
pddf:来源记录
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  "role": "pddf",
  "sha256": "f29d170d0059368497e53a6f4503ba5afaab57c662b3a975a384f1f9522139fe",
  "size": 53368,
  "status": "downloaded",
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sascif:来源记录
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  "role": "sascif",
  "status": "not_available",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXS7.sascif"
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summary:来源记录
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  "status": "downloaded",
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDXS7
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.titleStructures of nucleotide-bound Redondovirus Rep protein link conformation and function.
project.publication.author_listMontermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1013997
project.publication.pmid41779823
project.publication.published_date2026 Mar
project.statusreleased
project.submitted_date2025-08-11
project.released_date2026-02-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXS7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXS7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXS7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXS7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXS7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXS7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXS7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameID7A1 BioSAXS / HP-Bio Beamline
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman lung-associated vientovirus FB Replication-Associated Protein Dodecamer
experiment.sample.molecule[0].short_nameRep
experiment.sample.molecule[0].sequenceMTGGRLQNKNLYLTYLDTEERIFSELILITELAERLDKYGVKYAIACKEVAPSTGTVHYHCLICCENVISTRNGKELLTIENIMPHVGRIQNNLVNIVNYIKKDGSFAEVNKENAPKKKEMEKREKAELMINGNLRELFMEGVLGAIDIIRAEKLRNIFQVQAPADKYQKKLILWFKGETGEGKTKTAVDIAERFFNGEYWMSNETLKWFDGYRGQPVAIIDDFRKSMLTEWSFLLRLLDGYNLIVQVKGGFVKWQPKVIIVTSPASPSEAFQWVNKEGEIIEWDKQVQLTRRLTYEDEEQIYNFPLWNQDQLKLENTIRKYLGLAPMEEESMFPEEWSVIEPEGFITPG
experiment.sample.molecule[0].organismVientovirus FB
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last350
experiment.sample.molecule[0].oligomerizationdodecamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw40611.71
experiment.sample.molecule[0].total_mw487340.4
experiment.sample.molecule[0].number_molecules12
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionGenbank NC_055523.1
experiment.sample.buffer.name20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman lung-associated vientovirus FB Replication-Associated Protein ADP-bound (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameKushol
experiment.contributor[0].contributor_surnameGupta
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-7006-2667
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-01-25
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames500
experiment.wavelength1.10367
experiment.sample_detector_distance1.713
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume100.0
experiment.flow_rate0.5
experiment.s_min0.081
experiment.s_max4.88
experiment.total_exposure_timenull
experiment.seccolumn2
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw371.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw530.0
porod_mw_errornull
pddf_i06.32
pddf_i0_error0.05
guinier_i06.22115
guinier_i0_errornull
pddf_rg5.738
pddf_rg_error0.09
guinier_rg5.484
guinier_rg_error0.425
pddf_dmax239.0
pddf_dmax_errornull
porod_volume850.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first15
guinier_point_last64
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXS7_datloglog_img.png
symmetrynull
last_modified2026-03-16T15:52:53.563334+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXS7
statussuccess
started_at2026-08-11T15:38:26.994202+00:00
finished_at2026-08-11T15:38:34.896288+00:00
source_last_modified2026-03-16T15:52:53.563334+01:00
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files[0].size5778
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files[1].rolecurve
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files[4].rolefull_entry_zip
files[4].zip_member_count2
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDXS7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
    "publication": {
      "title": "Structures of nucleotide-bound Redondovirus Rep protein link conformation and function.",
      "author_list": "Montermoso S, Gupta K, Pumroy RA, Moiseenkova-Bell V, Bushman FD, Van Duyne GD",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1013997",
      "pmid": "41779823",
      "published_date": "2026 Mar"
    },
    "status": "released",
    "submitted_date": "2025-08-11",
    "released_date": "2026-02-16"
  },
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  "intensities_log_log_plot": "SASDXS7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-16T15:52:53.563334+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
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