SASDXT4

Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-11-07T14:21:29.048762+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus

浓度— – 1.0 缓冲液 / pH20 mM HEPES, 150 mM NaCl, 10% glycerol / 8.0
Experimental temperature4.0 设备 / 束线ESRF / BM29
波长0.0991 nm曝光2.0 s × 750

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
GTPase
查看序列
MDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
proteinMyxococcus xanthus (strain DK1622)Q1D0B61–120dimer分子数 215.948 kDa
Two-component system response regulator
查看序列
MDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH
proteinMyxococcus xanthusA0AAE6G1Y1328–421trimer分子数 312.89 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11000[3]0.06285053–5.209716 1/nm含误差列缺失 01sasbdb/entries/t4/sasdxt4/source/SASDXT4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.1nm
i0Guinier55.4756arbitrary
i0P(r)55.52arbitrary
mwExperimental49.0kDa
mwPorod49.0kDa
porod_volumePorod79.0nm³
rgGuinier2.5080.006nm
rgP(r)2.509nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM NaCl, 10% glycerol缓冲液浓度
pH8.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-03-31储存 / 测量温度-20.0 / 4.0
曝光时间2.0帧数750
波长0.0991样品-探测器距离2.81
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q range0.063 – 5.21样品体积 / 流速50.0 / 0.6

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/t4/sasdxt4/source/SASDXT4.outdownloaded57653e8ff3dfb3ffabeea8e0cc4b3836b18e01d4b7acfc38d39d90e6c07efbf197d60Download查看原文件源站
sascifsasbdb/entries/t4/sasdxt4/source/SASDXT4.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/t4/sasdxt4/source/summary.jsondownloaded7107c49f862e639de690c76db1d8b9a8494aa05546c226200ecda7234ed849eb0cafDownload查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDXT4
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStructural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus
project.publication.titleStructural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus
project.publication.author_listKodesia A, Kapoor S, Thakur K
project.publication.journalJournal of Biological Chemistry
project.publication.doi10.1016/j.jbc.2025.110907
project.publication.pmidnull
project.publication.published_date2025 Nov
project.statusreleased
project.submitted_date2025-05-18
project.released_date2025-11-07
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXT4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXT4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXT4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXT4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGTPase
experiment.sample.molecule[0].short_nameMglC
experiment.sample.molecule[0].sequenceMDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
experiment.sample.molecule[0].organismMyxococcus xanthus (strain DK1622)
experiment.sample.molecule[0].uniprot_codeQ1D0B6
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last120
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNA AETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
experiment.sample.molecule[0].mw15.948
experiment.sample.molecule[0].total_mw31.896
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionMutual gliding motility protein C. The protein construct contains an additional 25 non-native amino acids at the N-terminus, including a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS).
experiment.sample.molecule[1].long_nameTwo-component system response regulator
experiment.sample.molecule[1].short_nameRomR(328-421)
experiment.sample.molecule[1].sequenceMDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH
experiment.sample.molecule[1].organismMyxococcus xanthus
experiment.sample.molecule[1].uniprot_codeA0AAE6G1Y1
experiment.sample.molecule[1].uniprot_range_first328
experiment.sample.molecule[1].uniprot_range_last421
experiment.sample.molecule[1].oligomerizationtrimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMPKNLLVADDSLTIRKVIGMIFATEDFQVTAVDNGLDAISRTRELRPDVVLADVMMPGKS GYEVCEALKNDPATQGIPVVLLAGTFEAFDENRARAARADDHVTKPFESQVLLDKVKALV GQKSNTMPASAATQVRHAAPQPVAAPAPVAAAAPPGARPAPPPGARPGVPPGPGVPRPPP GAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMARPPGPGVPPGA RPPGPGMPPGARPGVPPPPGGSAPGLPPRPGMPPGAVARPGVPPPPGGPAPGGFSRPPVG APQPPPGAAPPPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQGDAEEISLDIATPAPVA ARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQ H
experiment.sample.molecule[1].mw12.89
experiment.sample.molecule[1].total_mw38.67
experiment.sample.molecule[1].number_molecules3
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionThe protein construct contains an addtional 25 non-native amino acids at the N-terminus that includes a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS).
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, 10% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameCSIR
experiment.contributor[0].affiliation[0].addressChandigarh, India
experiment.contributor[0].affiliation[0].full_nameCSIR-Institute of Microbial Technology
experiment.contributor[0].affiliation[0].webpagehttp://www.imtech.res.in/
experiment.contributor[0].contributor_nameAkriti
experiment.contributor[0].contributor_surnameKodesia
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-03-31
experiment.storage_temperature-20.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames750
experiment.wavelength0.0991
experiment.sample_detector_distance2.81
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volume50.0
experiment.flow_rate0.6
experiment.s_min0.063
experiment.s_max5.21
experiment.total_exposure_timenull
experiment.seccolumn34
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw49.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw49.0
porod_mw_errornull
pddf_i055.52
pddf_i0_errornull
guinier_i055.4756
guinier_i0_errornull
pddf_rg2.509
pddf_rg_errornull
guinier_rg2.508
guinier_rg_error0.006
pddf_dmax9.1
pddf_dmax_errornull
porod_volume79.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last88
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXT4_datloglog_img.png
symmetrynull
last_modified2025-11-07T14:21:29.048762+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXT4
statussuccess
started_at2026-08-11T14:31:15.362239+00:00
finished_at2026-08-11T14:31:23.064926+00:00
source_last_modified2025-11-07T14:21:29.048762+01:00
files[0].statusdownloaded
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查看完整 summary.json 原文
{
  "code": "SASDXT4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
    "publication": {
      "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
      "author_list": "Kodesia A, Kapoor S, Thakur K",
      "journal": "Journal of Biological Chemistry",
      "doi": "10.1016/j.jbc.2025.110907",
      "pmid": null,
      "published_date": "2025 Nov"
    },
    "status": "released",
    "submitted_date": "2025-05-18",
    "released_date": "2025-11-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXT4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXT4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_dat_img.png",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXT4_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXT4.sascif",
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        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
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      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "GTPase",
          "short_name": "MglC",
          "sequence": "MDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL",
          "organism": "Myxococcus xanthus (strain DK1622)",
          "uniprot_code": "Q1D0B6",
          "uniprot_range_first": 1,
          "uniprot_range_last": 120,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNA\nAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL",
          "mw": 15.948,
          "total_mw": 31.896,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Mutual gliding motility protein C. The protein construct contains an additional 25 non-native amino acids at the N-terminus, including a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS)."
        },
        {
          "long_name": "Two-component system response regulator",
          "short_name": "RomR(328-421)",
          "sequence": "MDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH",
          "organism": "Myxococcus xanthus",
          "uniprot_code": "A0AAE6G1Y1",
          "uniprot_range_first": 328,
          "uniprot_range_last": 421,
          "oligomerization": "trimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPKNLLVADDSLTIRKVIGMIFATEDFQVTAVDNGLDAISRTRELRPDVVLADVMMPGKS\nGYEVCEALKNDPATQGIPVVLLAGTFEAFDENRARAARADDHVTKPFESQVLLDKVKALV\nGQKSNTMPASAATQVRHAAPQPVAAPAPVAAAAPPGARPAPPPGARPGVPPGPGVPRPPP\nGAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMARPPGPGVPPGA\nRPPGPGMPPGARPGVPPPPGGSAPGLPPRPGMPPGAVARPGVPPPPGGPAPGGFSRPPVG\nAPQPPPGAAPPPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQGDAEEISLDIATPAPVA\nARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQ\nH",
          "mw": 12.89,
          "total_mw": 38.67,
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}
查看完整 manifest.json 原文
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