SASDXT4

Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2025-11-07T14:21:29.048762+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus

Concentration— – 1.0 Buffer / pH20 mM HEPES, 150 mM NaCl, 10% glycerol / 8.0
Experimental temperature4.0 Facility / beamlineESRF / BM29
Wavelength0.0991 nmExposure2.0 s × 750

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
GTPase
View sequence
MDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
proteinMyxococcus xanthus (strain DK1622)Q1D0B61–120dimerMolecule count 215.948 kDa
Two-component system response regulator
View sequence
MDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH
proteinMyxococcus xanthusA0AAE6G1Y1328–421trimerMolecule count 312.89 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11000[3]0.06285053–5.209716 1/nmHas uncertainty columnMissing 01sasbdb/entries/t4/sasdxt4/source/SASDXT4.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.1—nm
i0Guinier55.4756—arbitrary
i0P(r)55.52—arbitrary
mwExperimental49.0—kDa
mwPorod49.0—kDa
porod_volumePorod79.0—nm³
rgGuinier2.5080.006nm
rgP(r)2.509—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 150 mM NaCl, 10% glycerolBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-03-31Storage / measurement temperature-20.0 / 4.0
Exposure time2.0Frames750
Wavelength0.0991Sample-to-detector distance2.81
SourceX-ray synchrotronDetectorPilatus3 2M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.063 – 5.21Sample volume / flow rate50.0 / 0.6

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDXT4
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStructural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus
project.publication.titleStructural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus
project.publication.author_listKodesia A, Kapoor S, Thakur K
project.publication.journalJournal of Biological Chemistry
project.publication.doi10.1016/j.jbc.2025.110907
project.publication.pmidnull
project.publication.published_date2025 Nov
project.statusreleased
project.submitted_date2025-05-18
project.released_date2025-11-07
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXT4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXT4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXT4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXT4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameGTPase
experiment.sample.molecule[0].short_nameMglC
experiment.sample.molecule[0].sequenceMDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
experiment.sample.molecule[0].organismMyxococcus xanthus (strain DK1622)
experiment.sample.molecule[0].uniprot_codeQ1D0B6
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last120
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNA AETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL
experiment.sample.molecule[0].mw15.948
experiment.sample.molecule[0].total_mw31.896
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionMutual gliding motility protein C. The protein construct contains an additional 25 non-native amino acids at the N-terminus, including a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS).
experiment.sample.molecule[1].long_nameTwo-component system response regulator
experiment.sample.molecule[1].short_nameRomR(328-421)
experiment.sample.molecule[1].sequenceMDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH
experiment.sample.molecule[1].organismMyxococcus xanthus
experiment.sample.molecule[1].uniprot_codeA0AAE6G1Y1
experiment.sample.molecule[1].uniprot_range_first328
experiment.sample.molecule[1].uniprot_range_last421
experiment.sample.molecule[1].oligomerizationtrimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMPKNLLVADDSLTIRKVIGMIFATEDFQVTAVDNGLDAISRTRELRPDVVLADVMMPGKS GYEVCEALKNDPATQGIPVVLLAGTFEAFDENRARAARADDHVTKPFESQVLLDKVKALV GQKSNTMPASAATQVRHAAPQPVAAPAPVAAAAPPGARPAPPPGARPGVPPGPGVPRPPP GAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMARPPGPGVPPGA RPPGPGMPPGARPGVPPPPGGSAPGLPPRPGMPPGAVARPGVPPPPGGPAPGGFSRPPVG APQPPPGAAPPPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQGDAEEISLDIATPAPVA ARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQ H
experiment.sample.molecule[1].mw12.89
experiment.sample.molecule[1].total_mw38.67
experiment.sample.molecule[1].number_molecules3
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionThe protein construct contains an addtional 25 non-native amino acids at the N-terminus that includes a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS).
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, 10% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameCSIR
experiment.contributor[0].affiliation[0].addressChandigarh, India
experiment.contributor[0].affiliation[0].full_nameCSIR-Institute of Microbial Technology
experiment.contributor[0].affiliation[0].webpagehttp://www.imtech.res.in/
experiment.contributor[0].contributor_nameAkriti
experiment.contributor[0].contributor_surnameKodesia
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-03-31
experiment.storage_temperature-20.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames750
experiment.wavelength0.0991
experiment.sample_detector_distance2.81
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volume50.0
experiment.flow_rate0.6
experiment.s_min0.063
experiment.s_max5.21
experiment.total_exposure_timenull
experiment.seccolumn34
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw49.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw49.0
porod_mw_errornull
pddf_i055.52
pddf_i0_errornull
guinier_i055.4756
guinier_i0_errornull
pddf_rg2.509
pddf_rg_errornull
guinier_rg2.508
guinier_rg_error0.006
pddf_dmax9.1
pddf_dmax_errornull
porod_volume79.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last88
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXT4_datloglog_img.png
symmetrynull
last_modified2025-11-07T14:21:29.048762+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXT4
statussuccess
started_at2026-08-11T14:31:15.362239+00:00
finished_at2026-08-11T14:31:23.064926+00:00
source_last_modified2025-11-07T14:21:29.048762+01:00
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View the complete summary.json source
{
  "code": "SASDXT4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
    "publication": {
      "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
      "author_list": "Kodesia A, Kapoor S, Thakur K",
      "journal": "Journal of Biological Chemistry",
      "doi": "10.1016/j.jbc.2025.110907",
      "pmid": null,
      "published_date": "2025 Nov"
    },
    "status": "released",
    "submitted_date": "2025-05-18",
    "released_date": "2025-11-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXT4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXT4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXT4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXT4_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXT4.sascif",
  "experiment": {
    "instrument": {
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        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "GTPase",
          "short_name": "MglC",
          "sequence": "MDHHHHHHSSGVDLGTENLYFQSASMSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNAAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL",
          "organism": "Myxococcus xanthus (strain DK1622)",
          "uniprot_code": "Q1D0B6",
          "uniprot_range_first": 1,
          "uniprot_range_last": 120,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSFRTHLESVVNQVEGALACSVMGFDGISVDTFQKDESAELDLNGAWVEYANLLTQLRNA\nAETLKTGTVSEVSVNSEKVLTVMRLVSPDYFLVLALHADGNFGKGRYVLRVTAPKVRAEL",
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          "total_mw": 31.896,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Mutual gliding motility protein C. The protein construct contains an additional 25 non-native amino acids at the N-terminus, including a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS)."
        },
        {
          "long_name": "Two-component system response regulator",
          "short_name": "RomR(328-421)",
          "sequence": "MDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH",
          "organism": "Myxococcus xanthus",
          "uniprot_code": "A0AAE6G1Y1",
          "uniprot_range_first": 328,
          "uniprot_range_last": 421,
          "oligomerization": "trimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPKNLLVADDSLTIRKVIGMIFATEDFQVTAVDNGLDAISRTRELRPDVVLADVMMPGKS\nGYEVCEALKNDPATQGIPVVLLAGTFEAFDENRARAARADDHVTKPFESQVLLDKVKALV\nGQKSNTMPASAATQVRHAAPQPVAAPAPVAAAAPPGARPAPPPGARPGVPPGPGVPRPPP\nGAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMARPPGPGVPPGA\nRPPGPGMPPGARPGVPPPPGGSAPGLPPRPGMPPGAVARPGVPPPPGGPAPGGFSRPPVG\nAPQPPPGAAPPPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQGDAEEISLDIATPAPVA\nARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQ\nH",
          "mw": 12.89,
          "total_mw": 38.67,
          "number_molecules": 3,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct contains an addtional 25 non-native amino acids at the N-terminus that includes a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS)."
        }
      ],
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        "concentration_unit": null,
        "comment": null,
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        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
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    },
    "contributor": [
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        "affiliation": [
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View the complete manifest.json source
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