SASDXW3

Transthyretin tetramer

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-09-04T14:06:07.975288+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Transthyretin tetramer

Concentration— – 5.0 Buffer / pH20 mM Tris, 50 mM NaCl / 7.0
Experimental temperature26.0 Facility / beamlineTaiwan Photon Source, NSRRC / 13A
Wavelength0.08265 nmExposure2.0 s × 8

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Transthyretin
View sequence
GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE
proteinHomo sapiensP0276621–147tetramerMolecule count 413.761 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1570[3]0.0083403–1.552462 1/AHas uncertainty columnMissing 00sasbdb/entries/w3/sasdxw3/source/SASDXW3.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.2—Å
i0Guinier0.0747235—1/cm
i0P(r)0.07557—1/cm
mwExperimental58.7—kDa
mwPorod57.0—kDa
porod_volumePorod92.0—ų
rgGuinier2.4290.122Å
rgP(r)2.45—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris, 50 mM NaClBuffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2022-09-04Storage / measurement temperature26.0 / 26.0
Exposure time2.0Frames8
Wavelength0.08265Sample-to-detector distance2.5
SourceX-ray synchrotronDetectorEiger X 1M and Eiger X 9M
Facility / beamlineTaiwan Photon Source, NSRRC / 13A · Hsinchu, Taiwan
q range0.083 – 15.525Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDXW3
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDifferentiating the solution structures and stability of transthyretin tetramer complexed with tolcapone and tafamidis using SEC-SWAXS and NMR.
project.publication.titleDifferentiating the solution structures and stability of transthyretin tetramer complexed with tolcapone and tafamidis using SEC-SWAXS and NMR.
project.publication.author_listShih O, Feng YC, Agrawal S, Liao KF, Yeh YQ, Chang JW, Yu TY, Jeng US
project.publication.journalJ Appl Crystallogr
project.publication.doi10.1107/S1600576725004716
project.publication.pmid40765976
project.publication.published_date2025 Aug 1
project.statusreleased
project.submitted_date2025-04-13
project.released_date2025-04-24
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experiment.instrument.detector.nameEiger X 1M and Eiger X 9M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameTaiwan Photon Source, NSRRC
experiment.instrument.cityHsinchu
experiment.instrument.countryTaiwan
experiment.instrument.beamline_name13A
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameTransthyretin
experiment.sample.molecule[0].short_nameTTR
experiment.sample.molecule[0].sequenceGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP02766
experiment.sample.molecule[0].uniprot_range_first21
experiment.sample.molecule[0].uniprot_range_last147
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDT WEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDS GPRRYTIAALLSPYSYSTTAVVTNPKE
experiment.sample.molecule[0].mw13.761
experiment.sample.molecule[0].total_mw55.044
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM Tris, 50 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTransthyretin tetramer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHsinchu City, Taiwan
experiment.contributor[0].affiliation[0].full_nameNational Synchrotron Radiation Research Center
experiment.contributor[0].affiliation[0].webpagehttps://www.nsrrc.org.tw/
experiment.contributor[0].contributor_nameOrion
experiment.contributor[0].contributor_surnameShih
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
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experiment.storage_temperature26.0
experiment.cell_temperature26.0
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descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of transthyretin in 20 mM Tris, 50 mM NaCl, pH 7 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC (Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 0.3 m (Eiger X 1M) and 2.5 m (Eiger X 9M) and at a wavelength of f λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 26°C. Eight successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw58.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw57.0
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porod_volume_errornull
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estimated_volume_errornull
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symmetrynull
last_modified2025-09-04T14:06:07.975288+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDXW3
statussuccess
started_at2026-08-11T14:09:48.570185+00:00
finished_at2026-08-11T14:09:57.205316+00:00
source_last_modified2025-09-04T14:06:07.975288+02:00
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View the complete summary.json source
{
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  "status": "Published",
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      "title": "Differentiating the solution structures and stability of transthyretin tetramer complexed with tolcapone and tafamidis using SEC-SWAXS and NMR.",
      "author_list": "Shih O, Feng YC, Agrawal S, Liao KF, Yeh YQ, Chang JW, Yu TY, Jeng US",
      "journal": "J Appl Crystallogr",
      "doi": "10.1107/S1600576725004716",
      "pmid": "40765976",
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View the complete manifest.json source
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