SASDXX5

SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-09-01T15:11:14.414737+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

Concentration— – 0.5 Buffer / pH20 mM Tris, 150 mM NaCl / 7.5
Experimental temperature25.0 Facility / beamlineNational Synchrotron Light Source II (NSLS-II) / 16-ID (LiX)
Wavelength0.08189 nmExposure0.5 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Nucleoprotein
View sequence
MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
proteinSevere acute respiratory syndrome coronavirus 2P0DTC92–419dimerMolecule count 247.3 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1101[3]0.005–0.25 1/AHas uncertainty columnMissing 00sasbdb/entries/x5/sasdxx5/source/SASDXX5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)17.050.99Å
i0Guinier1.44685—arbitrary
i0P(r)1.14150.0023arbitrary
mwExperimental94.6—kDa
rgGuinier5.7680.17Å
rgP(r)5.7130.11Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris, 150 mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-07-18Storage / measurement temperature4.0 / 25.0
Exposure time0.5Frames10
Wavelength0.08189Sample-to-detector distance3.56
SourceX-ray synchrotronDetectorPilatus3 S 1M, Pilatus3 900 K
Facility / beamlineNational Synchrotron Light Source II (NSLS-II) / 16-ID (LiX) · Upton, NY, USA
q range0.05 – 2.5Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (1 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDXX5
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titlePhosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.
project.publication.titlePhosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.
project.publication.author_listFavetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-025-62922-4
project.publication.pmid40858555
project.publication.published_date2025 Aug 26
project.statusreleased
project.submitted_date2025-07-03
project.released_date2025-08-19
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.typeDuel detector
experiment.instrument.detector.namePilatus3 S 1M, Pilatus3 900 K
experiment.instrument.detector.resolutionnull
experiment.instrument.nameNational Synchrotron Light Source II (NSLS-II)
experiment.instrument.cityUpton, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_name16-ID (LiX)
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNucleoprotein
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
experiment.sample.molecule[0].organismSevere acute respiratory syndrome coronavirus 2
experiment.sample.molecule[0].uniprot_codeP0DTC9
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last419
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ QQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH WPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY KTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
experiment.sample.molecule[0].mw47.3
experiment.sample.molecule[0].total_mw94.6
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG). N-terminal His6xtag+tev cleavage site
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSARS-CoV-2 Nucleocapsid protein 0.5 mg/mL
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameRutgers University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameBruna
experiment.contributor[0].contributor_surnameFavetta
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-3613-1665
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-07-18
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames10
experiment.wavelength0.08189
experiment.sample_detector_distance3.56
experiment.concentration_minnull
experiment.concentration_max0.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.05
experiment.s_max2.5
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareBioXTAS RAW
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of SARS-CoV-2 Nucleocapsid protein in 20 mM Tris, 150 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors. One solute concentration of 0.50 mg/ml was measured at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw94.6
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i01.1415
pddf_i0_error0.0023
guinier_i01.44685
guinier_i0_errornull
pddf_rg5.713
pddf_rg_error0.11
guinier_rg5.768
guinier_rg_error0.17
pddf_dmax17.05
pddf_dmax_error0.99
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last19
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXX5_datloglog_img.png
symmetrynull
last_modified2025-09-01T15:11:14.414737+02:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDXX5
statussuccess
started_at2026-08-11T14:58:47.010700+00:00
finished_at2026-08-11T14:58:52.241664+00:00
source_last_modified2025-09-01T15:11:14.414737+02:00
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View the complete summary.json source
{
  "code": "SASDXX5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
    "publication": {
      "title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
      "author_list": "Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-62922-4",
      "pmid": "40858555",
      "published_date": "2025 Aug 26"
    },
    "status": "released",
    "submitted_date": "2025-07-03",
    "released_date": "2025-08-19"
  },
  "pddf_data": null,
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX5_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXX5.sascif",
  "experiment": {
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        "name": "Pilatus3 S 1M, Pilatus3 900 K",
        "resolution": null
      },
      "name": "National Synchrotron Light Source II (NSLS-II)",
      "city": "Upton, NY",
      "country": "USA",
      "beamline_name": "16-ID (LiX)",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Nucleoprotein",
          "short_name": null,
          "sequence": "MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
          "organism": "Severe acute respiratory syndrome coronavirus 2",
          "uniprot_code": "P0DTC9",
          "uniprot_range_first": 2,
          "uniprot_range_last": 419,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
          "mw": 47.3,
          "total_mw": 94.6,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG).\r\nN-terminal His6xtag+tev cleavage site"
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        "concentration_unit": null,
        "comment": null,
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      "purity_method": null,
      "name": "SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL",
      "ext_coefficient": null,
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      "absorbption": null,
      "deuteration": null,
      "mixture": null
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    "contributor": [
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            "address": null,
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            "webpage": null
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        "contributor_surname": "Favetta",
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    "concentration_unit": null,
    "date": "2023-07-18",
    "storage_temperature": 4.0,
    "cell_temperature": 25.0,
    "exposure_time": 0.5,
    "number_of_frames": 10,
    "wavelength": 0.08189,
    "sample_detector_distance": 3.56,
    "concentration_min": null,
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of SARS-CoV-2 Nucleocapsid protein in 20 mM Tris, 150 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA)  of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors. One solute concentration of 0.50 mg/ml was measured at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 94.6,
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View the complete manifest.json source
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