SASDXY4

Serratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-11-24T10:26:42.360507+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Serratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB

浓度— – 8.0 缓冲液 / pHphosphate-buffered saline / 7.4
Experimental temperature20.0 设备 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
波长0.1127 nm曝光0.1 s × 4

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB
查看序列
PWPANRYAVLAYHDVEDGAADQRYMSVRTSALNEQFAWLKQNGYQPVSVEQILQAQNGGPALPNKAVLLTFDDGYRSFYSRVFPLLKAYNWPAVLAPVGVWLDAPADKPVDFGGLMTPRDRFLTWQQVREVSDSGLVEIGAHTYASHYGGIANPQGNTEPAVANRLYDAKQGRYESEAEYRQRIEKDVALITQRITAATGKAPRVWVWPYGAAGGVALDIARKHGYRMALTLENGLADVNAPENVPRMLVAGNPSLTSFAQQLTQIQNRQIMRVAHVDLDYLYDPDPQQQAKNLDKLIQRIYDLRINTVFLQAFADPKGDGNVRELYFPNRWLPMKADLFNRVSWQLSSRTGVAVYAWMPVLAFELGSDVPRVQRLDPQNGQLSVDDKQYRRLSPFSPVARQRIGEIYEDLAAHATFKGILFHDDALLSDFEDAGPDAAAAYRQAGFADSVAAIRQDPQAMARWTRFKSRALIEFTQSLTERVRAIRGPQVQTARNIYAMPVLDPASEAWFGQNLADFLQAYDWVAPMAMPLMENVPRADSGRWLDQLVGKVAAYPGALDKTVFELQAVDWRKERRISLDDGQLLAEWMRRLQRNGAQSFGYYPDNFLDDRPK
proteinSerratia marcescensA0A2V4FWX540–653monomer分子数 169.149 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1942[3]0.006041352–0.5045199 1/A含误差列缺失 03sasbdb/entries/y4/sasdxy4/source/SASDXY4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.01Å
i0Guinier17.5918arbitrary
i0P(r)17.64arbitrary
mwExperimental65.7kDa
mwPorod65.7kDa
porod_volumePorod101.54ų
rgGuinier2.7520.028Å
rgP(r)2.77Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称phosphate-buffered saline缓冲液浓度
pH7.4添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-02-03储存 / 测量温度-80.0 / 20.0
曝光时间0.1帧数4
波长0.1127样品-探测器距离1.7
光源X-ray synchrotron探测器Pilatus3 X 1M
机构 / 束线Stanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q range0.06 – 5.045样品体积 / 流速0.05 / 0.05

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/y4/sasdxy4/source/SASDXY4.datdownloaded44625a1a983014c487f335b8154ec33b3fdc98b696f85ef218ee521ef3391ab8d5272Download查看原文件源站
full_entry_zipsasbdb/entries/y4/sasdxy4/source/SASDXY4.zipdownloaded136853ff8bbeaa533781fb2a6a0cd33f605ea9ca8f4a666813857f7c8b2cc30c73de78Download查看原文件源站
pddfsasbdb/entries/y4/sasdxy4/source/SASDXY4.outdownloaded77918fe51e8c600648e9748cb15a289ad17efd6de7a7db874a47d1631ee2e0e5eb068Download查看原文件源站
sascifsasbdb/entries/y4/sasdxy4/source/SASDXY4.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/y4/sasdxy4/source/summary.jsondownloaded84879d429115bac5b6a5c8744be42298a0d4c913da69c85944c37833f635676d3d62Download查看原文件源站
curve:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.dat",
  "role": "curve",
  "sha256": "a1a983014c487f335b8154ec33b3fdc98b696f85ef218ee521ef3391ab8d5272",
  "size": 44625,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDXY4.dat"
}
full_entry_zip:来源记录与 ZIP 内部目录(4 项)
{
  "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.zip",
  "role": "full_entry_zip",
  "sha256": "ff8bbeaa533781fb2a6a0cd33f605ea9ca8f4a666813857f7c8b2cc30c73de78",
  "size": 136853,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDXY4.zip",
  "zip_member_count": 4,
  "zip_members": [
    {
      "crc32": "4fbfe4cf",
      "name": "SASDXY4/experimental_data/SASDXY4.dat",
      "size": 44625
    },
    {
      "crc32": "f86544b6",
      "name": "SASDXY4/pddf/SASDXY4.out",
      "size": 77918
    },
    {
      "crc32": "63e680b2",
      "name": "SASDXY4/SASDXY4_fit1.fit",
      "size": 47397
    },
    {
      "crc32": "e8e95fdd",
      "name": "SASDXY4/SASDXY4_fit1_model1.pdb",
      "size": 396091
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.out",
  "role": "pddf",
  "sha256": "fe51e8c600648e9748cb15a289ad17efd6de7a7db874a47d1631ee2e0e5eb068",
  "size": 77918,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDXY4.out"
}
sascif:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.sascif",
  "role": "sascif",
  "status": "not_available",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXY4.sascif"
}
summary:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/summary.json",
  "sha256": "9d429115bac5b6a5c8744be42298a0d4c913da69c85944c37833f635676d3d62",
  "size": 8487,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDXY4/"
}

全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDXY4
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms
project.publication.titleStructural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms
project.publication.author_listAmanda Freitas Cruz
project.publication.journalUniversidade de São Paulo
project.publication.doinull
project.publication.pmidnull
project.publication.published_date2025 Sep 18
project.statusreleased
project.submitted_date2025-05-24
project.released_date2025-11-24
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDXY4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXY4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXY4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXY4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 1M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameStanford Synchrotron Radiation Lightsource (SSRL)
experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePoly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequencePWPANRYAVLAYHDVEDGAADQRYMSVRTSALNEQFAWLKQNGYQPVSVEQILQAQNGGPALPNKAVLLTFDDGYRSFYSRVFPLLKAYNWPAVLAPVGVWLDAPADKPVDFGGLMTPRDRFLTWQQVREVSDSGLVEIGAHTYASHYGGIANPQGNTEPAVANRLYDAKQGRYESEAEYRQRIEKDVALITQRITAATGKAPRVWVWPYGAAGGVALDIARKHGYRMALTLENGLADVNAPENVPRMLVAGNPSLTSFAQQLTQIQNRQIMRVAHVDLDYLYDPDPQQQAKNLDKLIQRIYDLRINTVFLQAFADPKGDGNVRELYFPNRWLPMKADLFNRVSWQLSSRTGVAVYAWMPVLAFELGSDVPRVQRLDPQNGQLSVDDKQYRRLSPFSPVARQRIGEIYEDLAAHATFKGILFHDDALLSDFEDAGPDAAAAYRQAGFADSVAAIRQDPQAMARWTRFKSRALIEFTQSLTERVRAIRGPQVQTARNIYAMPVLDPASEAWFGQNLADFLQAYDWVAPMAMPLMENVPRADSGRWLDQLVGKVAAYPGALDKTVFELQAVDWRKERRISLDDGQLLAEWMRRLQRNGAQSFGYYPDNFLDDRPK
experiment.sample.molecule[0].organismSerratia marcescens
experiment.sample.molecule[0].uniprot_codeA0A2V4FWX5
experiment.sample.molecule[0].uniprot_range_first40
experiment.sample.molecule[0].uniprot_range_last653
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMLIRSSLFVLAALLTTGCSQADVPRFTPPAERPVGPMERPWPANRYAVLAYHDVEDGAAD QRYMSVRTSALNEQFAWLKQNGYQPVSVEQILQAQNGGPALPNKAVLLTFDDGYRSFYSR VFPLLKAYNWPAVLAPVGVWLDAPADKPVDFGGLMTPRDRFLTWQQVREVSDSGLVEIGA HTYASHYGGIANPQGNTEPAVANRLYDAKQGRYESEAEYRQRIEKDVALITQRITAATGK APRVWVWPYGAAGGVALDIARKHGYRMALTLENGLADVNAPENVPRMLVAGNPSLTSFAQ QLTQIQNRQIMRVAHVDLDYLYDPDPQQQAKNLDKLIQRIYDLRINTVFLQAFADPKGDG NVRELYFPNRWLPMKADLFNRVSWQLSSRTGVAVYAWMPVLAFELGSDVPRVQRLDPQNG QLSVDDKQYRRLSPFSPVARQRIGEIYEDLAAHATFKGILFHDDALLSDFEDAGPDAAAA YRQAGFADSVAAIRQDPQAMARWTRFKSRALIEFTQSLTERVRAIRGPQVQTARNIYAMP VLDPASEAWFGQNLADFLQAYDWVAPMAMPLMENVPRADSGRWLDQLVGKVAAYPGALDK TVFELQAVDWRKERRISLDDGQLLAEWMRRLQRNGAQSFGYYPDNFLDDRPKLDAVRPVL SSAWFPLP
experiment.sample.molecule[0].mw69.149
experiment.sample.molecule[0].total_mw69.149
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionSerratia marcescens PgaB withoud two disordered regions in the N- and C-terminals.
experiment.sample.buffer.namephosphate-buffered saline
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSerratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressSão Paulo, SP, Brazil
experiment.contributor[0].affiliation[0].full_nameInstituto de Física, Universidade de São Paulo
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameAmanda
experiment.contributor[0].contributor_surnameFreitas Cruz
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-02-03
experiment.storage_temperature-80.0
experiment.cell_temperature20.0
experiment.exposure_time0.1
experiment.number_of_frames4
experiment.wavelength0.1127
experiment.sample_detector_distance1.7
experiment.concentration_minnull
experiment.concentration_max8.0
experiment.sample_volume0.05
experiment.flow_rate0.05
experiment.s_min0.06
experiment.s_max5.045
experiment.total_exposure_timenull
experiment.seccolumn1
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDXY4_fit1_model1_img.png
fits[0].models[0].softwareAlphaFold
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDXY4_fit1_model1.pdb
fits[0].models[0].model_mw69.1
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetryP1
fits[0].models[0].commentnull
fits[0].models[0].user1234
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXY4_fit1_fixed_fit_img.png
fits[0].softwareCRYSOL
fits[0].chi_square_value4.273
fits[0].p_value0.0
fits[0].fit_residual_plotSASDXY4_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDXY4_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.1
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of truncated PgaB in phosphate-buffered saline, pH 7.4 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 0.05 μl sample at 8 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. Four successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw65.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw65.7
porod_mw_errornull
pddf_i017.64
pddf_i0_errornull
guinier_i017.5918
guinier_i0_errornull
pddf_rg2.77
pddf_rg_errornull
guinier_rg2.752
guinier_rg_error0.028
pddf_dmax9.01
pddf_dmax_errornull
porod_volume101.54
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last78
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXY4_datloglog_img.png
symmetrynull
last_modified2025-11-24T10:26:42.360507+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDXY4
statussuccess
started_at2026-08-11T14:34:49.237594+00:00
finished_at2026-08-11T14:34:57.501731+00:00
source_last_modified2025-11-24T10:26:42.360507+01:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDXY4/
files[0].path/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/summary.json
files[0].size8487
files[0].sha2569d429115bac5b6a5c8744be42298a0d4c913da69c85944c37833f635676d3d62
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDXY4.dat
files[1].path/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.dat
files[1].size44625
files[1].sha256a1a983014c487f335b8154ec33b3fdc98b696f85ef218ee521ef3391ab8d5272
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDXY4.out
files[2].path/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.out
files[2].size77918
files[2].sha256fe51e8c600648e9748cb15a289ad17efd6de7a7db874a47d1631ee2e0e5eb068
files[2].rolepddf
files[3].statusnot_available
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDXY4.sascif
files[3].path/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.sascif
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDXY4.zip
files[4].path/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.zip
files[4].size136853
files[4].sha256ff8bbeaa533781fb2a6a0cd33f605ea9ca8f4a666813857f7c8b2cc30c73de78
files[4].rolefull_entry_zip
files[4].zip_member_count4
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDXY4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms",
    "publication": {
      "title": "Structural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms",
      "author_list": "Amanda Freitas Cruz",
      "journal": "Universidade de São Paulo",
      "doi": null,
      "pmid": null,
      "published_date": "2025 Sep 18"
    },
    "status": "released",
    "submitted_date": "2025-05-24",
    "released_date": "2025-11-24"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXY4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXY4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXY4_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXY4_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXY4.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 1M",
        "resolution": 0.172
      },
      "name": "Stanford Synchrotron Radiation Lightsource (SSRL)",
      "city": "Menlo Park, CA",
      "country": "USA",
      "beamline_name": "BL4-2",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB",
          "short_name": null,
          "sequence": "PWPANRYAVLAYHDVEDGAADQRYMSVRTSALNEQFAWLKQNGYQPVSVEQILQAQNGGPALPNKAVLLTFDDGYRSFYSRVFPLLKAYNWPAVLAPVGVWLDAPADKPVDFGGLMTPRDRFLTWQQVREVSDSGLVEIGAHTYASHYGGIANPQGNTEPAVANRLYDAKQGRYESEAEYRQRIEKDVALITQRITAATGKAPRVWVWPYGAAGGVALDIARKHGYRMALTLENGLADVNAPENVPRMLVAGNPSLTSFAQQLTQIQNRQIMRVAHVDLDYLYDPDPQQQAKNLDKLIQRIYDLRINTVFLQAFADPKGDGNVRELYFPNRWLPMKADLFNRVSWQLSSRTGVAVYAWMPVLAFELGSDVPRVQRLDPQNGQLSVDDKQYRRLSPFSPVARQRIGEIYEDLAAHATFKGILFHDDALLSDFEDAGPDAAAAYRQAGFADSVAAIRQDPQAMARWTRFKSRALIEFTQSLTERVRAIRGPQVQTARNIYAMPVLDPASEAWFGQNLADFLQAYDWVAPMAMPLMENVPRADSGRWLDQLVGKVAAYPGALDKTVFELQAVDWRKERRISLDDGQLLAEWMRRLQRNGAQSFGYYPDNFLDDRPK",
          "organism": "Serratia marcescens",
          "uniprot_code": "A0A2V4FWX5",
          "uniprot_range_first": 40,
          "uniprot_range_last": 653,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MLIRSSLFVLAALLTTGCSQADVPRFTPPAERPVGPMERPWPANRYAVLAYHDVEDGAAD\nQRYMSVRTSALNEQFAWLKQNGYQPVSVEQILQAQNGGPALPNKAVLLTFDDGYRSFYSR\nVFPLLKAYNWPAVLAPVGVWLDAPADKPVDFGGLMTPRDRFLTWQQVREVSDSGLVEIGA\nHTYASHYGGIANPQGNTEPAVANRLYDAKQGRYESEAEYRQRIEKDVALITQRITAATGK\nAPRVWVWPYGAAGGVALDIARKHGYRMALTLENGLADVNAPENVPRMLVAGNPSLTSFAQ\nQLTQIQNRQIMRVAHVDLDYLYDPDPQQQAKNLDKLIQRIYDLRINTVFLQAFADPKGDG\nNVRELYFPNRWLPMKADLFNRVSWQLSSRTGVAVYAWMPVLAFELGSDVPRVQRLDPQNG\nQLSVDDKQYRRLSPFSPVARQRIGEIYEDLAAHATFKGILFHDDALLSDFEDAGPDAAAA\nYRQAGFADSVAAIRQDPQAMARWTRFKSRALIEFTQSLTERVRAIRGPQVQTARNIYAMP\nVLDPASEAWFGQNLADFLQAYDWVAPMAMPLMENVPRADSGRWLDQLVGKVAAYPGALDK\nTVFELQAVDWRKERRISLDDGQLLAEWMRRLQRNGAQSFGYYPDNFLDDRPKLDAVRPVL\nSSAWFPLP",
          "mw": 69.149,
          "total_mw": 69.149,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Serratia marcescens PgaB withoud two disordered regions in the N- and C-terminals."
        }
      ],
      "buffer": {
        "name": "phosphate-buffered saline",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Serratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "São Paulo, SP, Brazil",
            "full_name": "Instituto de Física, Universidade de São Paulo",
            "webpage": ""
          }
        ],
        "contributor_name": "Amanda",
        "contributor_surname": "Freitas Cruz",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-02-03",
    "storage_temperature": -80.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.1,
    "number_of_frames": 4,
    "wavelength": 0.1127,
    "sample_detector_distance": 1.7,
    "concentration_min": null,
    "concentration_max": 8.0,
    "sample_volume": 0.05,
    "flow_rate": 0.05,
    "s_min": 0.06,
    "s_max": 5.045,
    "total_exposure_time": null,
    "seccolumn": 1
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDXY4_fit1_model1_img.png",
          "software": "AlphaFold",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDXY4_fit1_model1.pdb",
          "model_mw": 69.1,
          "bead_radius": null,
          "log": null,
          "symmetry": "P1",
          "comment": null,
          "user": 1234
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXY4_fit1_fixed_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 4.273,
      "p_value": 0.0,
      "fit_residual_plot": "SASDXY4_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDXY4_fit1.fit",
      "fit_log": null,
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.1",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of truncated PgaB in phosphate-buffered saline, pH 7.4 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 0.05 μl sample at 8 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. Four successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 65.7,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 65.7,
  "porod_mw_error": null,
  "pddf_i0": 17.64,
  "pddf_i0_error": null,
  "guinier_i0": 17.5918,
  "guinier_i0_error": null,
  "pddf_rg": 2.77,
  "pddf_rg_error": null,
  "guinier_rg": 2.752,
  "guinier_rg_error": 0.028,
  "pddf_dmax": 9.01,
  "pddf_dmax_error": null,
  "porod_volume": 101.54,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 12,
  "guinier_point_last": 78,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXY4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-11-24T10:26:42.360507+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDXY4",
  "status": "success",
  "started_at": "2026-08-11T14:34:49.237594+00:00",
  "finished_at": "2026-08-11T14:34:57.501731+00:00",
  "source_last_modified": "2025-11-24T10:26:42.360507+01:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDXY4/",
      "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/summary.json",
      "size": 8487,
      "sha256": "9d429115bac5b6a5c8744be42298a0d4c913da69c85944c37833f635676d3d62"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDXY4.dat",
      "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.dat",
      "size": 44625,
      "sha256": "a1a983014c487f335b8154ec33b3fdc98b696f85ef218ee521ef3391ab8d5272",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDXY4.out",
      "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.out",
      "size": 77918,
      "sha256": "fe51e8c600648e9748cb15a289ad17efd6de7a7db874a47d1631ee2e0e5eb068",
      "role": "pddf"
    },
    {
      "status": "not_available",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXY4.sascif",
      "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.sascif",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDXY4.zip",
      "path": "/data/saxsdb/sasbdb/entries/y4/sasdxy4/source/SASDXY4.zip",
      "size": 136853,
      "sha256": "ff8bbeaa533781fb2a6a0cd33f605ea9ca8f4a666813857f7c8b2cc30c73de78",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}